Journal
BIOMEDICAL MICRODEVICES
Volume 13, Issue 6, Pages 1053-1062Publisher
SPRINGER
DOI: 10.1007/s10544-011-9575-x
Keywords
Antibody detection; Biosensor; LIPS; Microfluidics
Funding
- National Institutes of Health
- National Institute of Biomedical Imaging and Bioengineering
- National Institute of Dental and Craniofacial Research
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There is great interest in point-of-care antibody testing for the diagnosis of infectious and autoimmune diseases. As a first step in the development of self-contained and miniaturized devices for highly quantitative antibody detection, we demonstrate the application of Luciferase Immunoprecipitation Systems (LIPS) technology in a microfluidic format. Protein A/G was immobilized on the walls of PDMS-glass microchannels of 500 nL volume. The assay proceeds with the simultaneous introduction of plasma and Renilla luciferase-tagged antigens. Following washing, coelenterazine substrate was added and bound antigen-luciferase measured by chemiluminescence. Total assay time, including rinsing and detection, is under 10 min. Using these stable microfluidic devices, high diagnostic performance (100% sensitivity and 100% specificity) was achieved for the diagnosis of HSV-2 infection. Based on these findings, the LIPS microfluidic format should readily lend itself to automation and the transfer to portable instrumentation.
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