Article
Biochemistry & Molecular Biology
Calla B. Shubin, Rini Mayangsari, Ariel D. Swett, Carol W. Greider
Summary: In budding yeast, Rif1 negatively regulates telomere length through a region with HEAT repeats that mediate telomere length regulation, separate from its proposed DNA binding function. Conserved amino acids from position 436 to 577 play a crucial role in telomere length regulation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Cameron M. Robertson, Yuan Xue, Shobir Chowdhury, Laura Maringele
Summary: Rif1 is involved in telomere length regulation, DNA replication, and DNA damage responses in budding yeast. We identified a novel phosphorylation of Rif1 during telomere damage, and serines 57 and 110 within a phospho-gate domain (PGD) were found to be important for this modification. The phosphorylation of Rif1 inhibits its accumulation on damaged chromosomes and the proliferation of cells with telomere damage.
MOLECULAR AND CELLULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yasuhiro Yamamoto, Haruka Chino, Satoshi Tsukamoto, Koji L. Ode, Hiroki R. Ueda, Noboru Mizushima
Summary: This study demonstrates that NEK9 interacts with MYH9 to regulate ciliogenesis, with NEK9's LIR playing a key role in the autophagic degradation of MYH9. This interaction may have adaptive implications for terrestrial life, given the conservation of NEK9's LIR only in land vertebrates.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Chengjun Cao, Keyi Wang, Yina Wang, Tong-Bao Liu, Amariliz Rivera, Chaoyang Xue
Summary: The pathogenic yeast Cryptococcus neoformans forms large titan cells during infection. This process is regulated by multiple signaling pathways, with phosphorylation of Gpa1 by Crk1 being involved in titan cell formation. Fbp1, an E3 ubiquitin ligase, inhibits the formation of titan cells by targeting the degradation of Crk1. Strains overproducing titan cells show reduced virulence in a mouse model.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Karen Voelkel-Meiman, Ashwini Oke, Arden Feil, Alexander Shames, Jennifer Fung, Amy J. MacQueen
Summary: The study reveals that while the SC proteins are not essential for recombination itself, they promote efficient mismatch repair at interhomolog recombination sites. Failure to repair mismatches leads to the formation of genotypically sectored colonies, with an increased frequency of unrepaired mismatches in cells lacking specific proteins.
Article
Biochemistry & Molecular Biology
Michael C. Lanz, Kumar Yugandhar, Shagun Gupta, Ethan J. Sanford, Vitor M. Faca, Stephanie Vega, Aaron M. N. Joiner, J. Christopher Fromme, Haiyuan Yu, Marcus B. Smolka
Summary: This study assembled a dataset of 30,902 phosphosites from 75 independent phosphoproteomic experiments using Saccharomyces cerevisiae. By aggregating this dataset with others, over 46,000 budding yeast phosphosites were identified. Computational positioning of phosphorylation sites on protein structures revealed numerous sites predicted to disrupt protein complex architecture, providing insights into potential functional phosphorylation events.
Review
Genetics & Heredity
Jinbo Fan, Zhuo Sun, Yang Wang
Summary: The LINC complex serves as a link between the nucleoskeleton and cytoskeleton, with SUN and KASH proteins playing key roles in transmitting forces across the nuclear envelope. Research in budding yeast has led to the identification of a telomere-associated LINC complex, providing insights into potential LINC variants in other eukaryotes.
Article
Chemistry, Multidisciplinary
Fan Yu, Song Yang, Hua Ni, Dai Heng, Xuemei Wu, Mulin Yang, Xinming Zhang, Yuxin Cao, Yandong Pei, Di An, Dengwen Li, Dayong Liu, Lin Liu, Leiting Pan, Quan Chen, Xueliang Zhu, Jun Zhou
Summary: This study constructs a mouse model with Ogt gene knockout and demonstrates the important role of O-GlcNAcylation in establishing and maintaining cell polarity. The research finds that O-GlcNAcylation promotes the centrosomal localization of PCM1 and CEP131, maintaining the interaction between microtubules and the centrosome for cell polarity.
Article
Biochemistry & Molecular Biology
Masafumi Hayashi, Kenji Keyamura, Asami Yoshida, Mariko Ariyoshi, Genki Akanuma, Takashi Hishida
Summary: Histone H3/H4 mutations termed SDD mutations were found to suppress DNA damage sensitivity in DDT-deficient cells, indicating a link between nucleosome functions, chromatin remodeling, DNA damage tolerance (DDT), and homologous recombination (HR). These mutations led to reduced levels of PCNA ubiquitination and impaired recovery from replication blockage, highlighting the importance of INO80-dependent chromatin remodeling in regulating DDT and HR.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Cell Biology
Gabriel M. Gihana, Arthur A. Cross-Najafi, Soni Lacefield
Summary: In budding yeast, the Cdc42 GTPase establishes a polar front during G1, with the activity of the Cdc14 phosphatase released through the mitotic exit network playing a crucial role in redistributing Cdc42 between mother and daughter cells. The interaction between Bem3, a Cdc42 GAP, and Cdc14 is important for the dephosphorylation and activation of Bem3, allowing for the inactivation and redistribution of Cdc42. These findings reveal a mechanism by which Cdc14 regulates the spatial and temporal activity of Cdc42 to maintain normal cell size at cytokinesis.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Kimberly K. Fong, Trisha N. Davis, Charles L. Asbury
Summary: To assemble a bipolar spindle, microtubules need to bundle into an antiparallel midzone, which can be achieved by overcoming the initial preference for parallel interactions through pivoting. Pivoting flexibility of microtubules around spindle poles is crucial for timely pole separation, with passive thermal pivoting aiding initial contact and active minus end-directed force generation required for antiparallel alignment.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, Ryan Muller, Rachel Baum, Nicholas T. Ingolia
Summary: This study introduces a comprehensive yeast CRISPRi library based on empirical design rules, which has been validated to provide comprehensive genome coverage through competitive growth experiments. The fitness defects of essential genes caused by different guides targeting them were used to refine yeast CRISPRi design rules, and guide abundance measurements in pooled screens were improved by linking guides with random nucleotide barcodes and carrying out linear amplification.
Article
Genetics & Heredity
Majdi M. Kabaha, Yehuda Tzfati
Summary: Telomeres are specialized nucleoprotein complexes that protect the ends of eukaryotic chromosomes. Disruption of telomere protection may lead to aging-associated pathologies and cancer. Research shows that the protein Rap1 plays a critical role in telomere protection by negatively regulating telomere length and repressing homologous recombination and NHEJ, with other redundant pathways also contributing to telomere integrity and cell viability.
Article
Cell Biology
Yi-Wei Wang, Shu-Chuan Chen, De-Leung Gu, Yi-Chen Yeh, Jhih-Jie Tsai, Kuo-Tai Yang, Yuh-Shan Jou, Teh-Ying Chou, Tang K. Tang
Summary: The study investigates the expression levels of centriolar/centrosomal genes in various types of cancers and identifies STIL as a protein that is highly expressed in lung and other types of cancers. Depletion of STIL inhibits tumor growth and metastasis, while excess STIL activates the EMT pathway and enhances cancer cell migration and invasion. The study reveals an unexpected role of STIL in tumor metastasis and identifies its association with FOXM1 in promoting metastasis and stemness.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Stephen R. Wellard, Yujiao Zhang, Chris Shults, Xueqi Zhao, Matthew McKay, Stephen A. Murray, Philip W. Jordan
Summary: The study highlights the critical role of centriole duplication, centrosome maturation, and separation in establishing bipolar spindles during meiotic divisions. The findings show that Polo-like kinase 1 and Aurora A kinase are essential for centrosome maturation and separation, and PLK1 is required to inhibit the second round of centriole duplication until late anaphase I to ensure accurate chromosome segregation during spermatogenesis.
Article
Genetics & Heredity
Ping Li, Hui Jin, Bailey A. Koch, Rebecca L. Abblett, Xuemei Han, John R. Yates, Hong-Guo Yu
Article
Biochemistry & Molecular Biology
Ping Li, Hui Jin, Margaret L. Hoang, Hong-Guo Yu
CHROMOSOME RESEARCH
(2011)
Article
Genetics & Heredity
Ilana L. Brito, Hong-Guo Yu, Angelika Amon
Article
Genetics & Heredity
Weiqiang Lin, Mian Wang, Hui Jin, Hong-Guo Yu
Article
Cell Biology
Hui Jin, Vincent Guacci, Hong-Guo Yu
JOURNAL OF CELL BIOLOGY
(2009)
Article
Cell Biology
Katelan Shirk, Hui Jin, Thomas H. Giddings, Mark Winey, Hong-Guo Yu
JOURNAL OF CELL SCIENCE
(2011)
Article
Cell Biology
Weiqiang Lin, Hui Jin, Xiuwen Liu, Kristin Hampton, Hong-Guo Yu
MOLECULAR BIOLOGY OF THE CELL
(2011)
Article
Cell Biology
Ping Li, Hui Jin, Hong-Guo Yu
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Cell Biology
Meenakshi Agarwal, Hui Jin, Melainia McClain, Jinbo Fan, Bailey A. Koch, Sue L. Jaspersen, Hong-Guo Yu
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Genetics & Heredity
Fengzhi Jin, Hong Liu, Ping Li, Hong-Guo Yu, Yanchang Wang
Article
Cell Biology
Bailey A. Koch, Hui Jin, Robert J. Tomko, Hong-Guo Yu
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biology
Jinbo Fan, Hui Jin, Bailey A. Koch, Hong-Guo Yu
LIFE SCIENCE ALLIANCE
(2020)
Article
Cell Biology
Bailey A. Koch, Elizabeth Staley, Hui Jin, Hong-Guo Yu
Review
Cell Biology
Bailey Koch, Hong-Guo Yu