Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, Alain da Silva Morais, Katia A. Mesquita, Rogerio P. Pirraco, Rui L. Reis, Vitor M. Conrelo
Summary: We developed an injectable gellan gum-based hydrogel that delivers C2C12 into a localized myopathic model. The gellan gum was biofunctionalized with a laminin-derived peptide to mimic the natural muscular extracellular matrix. Furthermore, the hydrogel was physically adjusted to replicate the mechanical properties of native tissue. This formula was utilized for the first time in the field of skeletal muscle tissue regeneration. The injectability of the hydrogel allowed for non-invasive administration, creating a reliable microenvironment that supported C2C12 with minimal inflammation. The treatment of skeletal muscle defects with the cell-laden hydrogel significantly enhanced regeneration of localized muscular trauma.
ACTA BIOMATERIALIA
(2022)
Article
Agriculture, Dairy & Animal Science
Savannah R. Gregg, Madison R. Barshick, Sally E. Johnson
Summary: The study found that injection of hyaluronic acid can have anti-inflammatory effects without disrupting the normal muscle repair process after exercise.
Article
Biochemistry & Molecular Biology
Jae Yoon An, Wooram Um, Dong Gil You, Yeari Song, Jeongjin Lee, Nguyen Van Quy, Hyeyeon Joo, Jueun Jeon, Jae Hyung Park
Summary: A novel hyaluronic acid hydrogel-based depot with ultrasound-triggered thermal elevation and on-demand cargo release for cancer therapy was reported in this study. The results demonstrated that the hydrogel significantly increased temperature and released cargo under ultrasound, showing potential for in vivo applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Yuning Luo, Junyan Tan, Yue Zhou, Yuqiong Guo, Xinying Liao, Li He, Dingxilei Li, Xinxin Li, Yang Liu
Summary: Hyaluronic acid (HA) is a natural polysaccharide with excellent biocompatibility and moisturizing effect, which can affect angiogenesis, inflammation, and cell behavior. HA-based hydrogels formed by physical or chemical crosslinking strategies have both the physiological function of HA and the skeleton function of hydrogel. However, natural HA-based hydrogels have limitations such as insufficient mechanical strength and susceptibility to degradation. Researchers have developed various HA-based multifunctional hydrogels with notable properties by modifying the structure, using novel crosslinking strategies, and introducing reactive molecules. These hydrogels have extended the application scope in anticancer drug delivery, cartilage repair, three-dimensional cell culture, skin dressing, and other fields.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Jeong Ho Lim, Mirza Masroor Ali Beg, Khurshid Ahmad, Sibhghatulla Shaikh, Syed Sayeed Ahmad, Hee Jin Chun, Dukhwan Choi, Woo-Jong Lee, Jun-O Jin, Jihoe Kim, Arif Tasleem Jan, Eun Ju Lee, Inho Choi
Summary: The study shows that IgLON5 plays a crucial role in myogenesis and muscle regeneration by interacting with extracellular matrix and cell membrane proteins, creating an essential microenvironment for muscle stem cell survival.
Article
Engineering, Biomedical
Miranda M. Carleton, Marius Locke, Michael V. Sefton
Summary: The study demonstrates that MAA-collagen hydrogel significantly enhances skeletal muscle regeneration after traumatic injury, increasing muscle fiber size and force production while reducing pro-inflammatory macrophages. The regenerative effects of the hydrogels are carrier-dependent with distinct gene expression profiles.
Article
Biochemistry & Molecular Biology
Sheida Jahanbekam, Negin Mozafari, Azar Bagheri-Alamooti, Soliman Mohammadi-Samani, Saeid Daneshamouz, Reza Heidari, Negar Azarpira, Hajar Ashrafi, Amir Azadi
Summary: An injectable thermo-ultrasound-triggered drug carrier based on Pluronic® F-127, hyaluronic acid, and gelatin was designed for the treatment of arthritis. The thermo-responsive hydrogel showed a prolonged drug release and improved the disease status in an animal model. This study highlights the potential of ultrasound-responsive in situ-forming hydrogels for efficient treatment of arthritis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shuqin Yu, Shuo Wang, Lixin Xia, Huiwen Hu, Mingyu Zou, Zhiwen Jiang, Jinhua Chi, Yijie Zhang, Hongjian Li, Chaozhong Yang, Wanshun Liu, Baoqin Han
Summary: A study has successfully prepared a thiolated hyaluronic acid (SH-HA) hydrogel as a vitreous substitute, which demonstrated excellent biocompatibility and intraocular compatibility in experimental rabbits.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Sheila Maiz-Fernandez, Nagore Barroso, Leyre Perez-Alvarez, Unai Silvan, Jose Luis Vilas-Vilela, Senentxu Lanceros-Mendez
Summary: This study evaluates the various properties of HA/CHI polycomplex hydrogels and optimizes their synthesis parameters, demonstrating their potential in advanced biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Dairy & Animal Science
Kai-Zhi Zhou, Peng-Fei Wu, Xuan-Ze Ling, Jin Zhang, Qi-Fan Wang, Xin-Chao Zhang, Qian Xue, Tao Zhang, Wei Han, Gen-Xi Zhang
Summary: This study demonstrated that miR-460b-5p positively regulates the proliferation and differentiation of chicken myoblasts and that RBM19 is a target gene of miR-460b-5p. These findings are important for understanding the regulatory mechanism of meat development in broilers.
Article
Biochemistry & Molecular Biology
Xianxian Zhang, Shihui Huang, Xi Niu, Sheng Li, Jiafu Wang, Xueqin Ran
Summary: In this study, it was discovered that miR-103-3p is highly expressed in skeletal muscle and has inhibitory effects on myotube formation and differentiation of C2C12 cells. Furthermore, miR-103-3p was found to directly target the MAP4 gene and regulate autophagy in myoblasts. These findings contribute to a better understanding of the regulatory role of miRNAs in skeletal muscle myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Kaizhi Ding, Yanchun Gong, Chunfang Cheng, Xiaonuo Li, Yuanjie Zhu, Xiaofei Gao, Yuhua Li, Chunhua Yuan, Zhibing Liu, Wei Jiang, Chong Chen, Li-Hua Yao
Summary: This study investigates the role of voltage-gated sodium channels (VGSC) in muscle regeneration. The findings suggest that with the progression of differentiation, VGSC expression and intracellular sodium concentration increase, and VGSC dynamics change, potentially contributing to muscle contraction.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Simona Zanotti, Michela Ripolone, Laura Napoli, Daniele Velardo, Sabrina Salani, Patrizia Ciscato, Silvia Priori, Deni Kukavica, Andrea Mazzanti, Luca Diamanti, Elisa Vegezzi, Maurizio Moggio, Stefania Corti, Giacomo Comi, Monica Sciacco
Summary: Phospholamban plays a role in regulating calcium activity and storage in cardiac muscle. This study investigated the effect of PLN gene mutations in skeletal muscle and found histological and functional alterations, suggesting a potential association between PLN mutations and skeletal muscle involvement. Further studies are needed to understand the pathomechanism and potential therapeutic options for PLN-related skeletal muscle disorders.
Article
Biochemistry & Molecular Biology
Ailma O. da Paixao, Anaysa Paola Bolin, Joao G. Silvestre, Alice Cristina Rodrigues
Summary: The saturated fatty acid palmitic acid negatively affects muscle tube diameter, fusion, and metabolism, which may be related to miR-206.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
