Review
Chemistry, Analytical
Isabela de Paula Lima, Sandy Polycarpo Valle, Marcone Augusto Leal de Oliveira, Flavia Ferreira de Carvalho Marques, Fernando Antonio Simas Vaz
Summary: Monolithic stationary phases have been widely studied in separation science, particularly in liquid chromatography and capillary electrochromatography, for over three decades. The production of monolithic columns involves various steps, including precursor solution preparation, tubing pretreatment, solution introduction, in situ reaction, and posttreatment. Different types of processes and reactions, such as sol-gel, polycondensation, free radical polymerization, and click reactions, are discussed. This work aims to provide an overview of the production process and the characteristics of monolith compositions, as well as to encourage researchers in finding suitable monoliths for analytical separations.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Analytical
Rustem Kecili, Fatemeh Ghorbani-Bidkorbeh, Ibrahim Dolak, Gurbet Canpolat, Muharrem Karabork, Chaudhery Mustansar Hussain
Summary: This paper introduces the superior features of nanomaterials, especially magnetic nanoparticles, and highlights the latest progress and applications in their development for extraction and chromatographic applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Rui Zong, Han Yin, Yuhong Xiang, Lu Zhang, Nengsheng Ye
Summary: A fluorinated covalent organic framework, TFA-TAPB, was proposed for electrochromatographic separation. The TFA-TAPB-coated capillary columns exhibited satisfactory separation performance and reproducibility, indicating high potential for application in CEC separation.
Review
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This paper provides a detailed overview of recent developments in separation and analysis of proteins using capillary electromigration (CE) methods. It discusses sample preparation, suppression of protein sorption, control of electroosmotic flow, specific CE methods for protein separation, and protein identification and quantification. Additionally, it showcases several novel applications of CE methods in protein analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Genlin Sun, Dong Min Choi, Helong Xu, Sung Hyeon Baeck, Kyung Ho Row, Weiyang Tang
Summary: A novel chiral porous column was fabricated by immobilizing lipase on MIL-100(Fe) biocomposites, which were used as a chiral stationary phase for capillary electrochromatographic enantioseparation. The lipase biocomposites based on MOFs not only improved the stereoselective activities, but also enhanced the stability and applicability of the enzyme. The performance of the porous column was characterized and evaluated, showing high resolution and excellent stability for enantioseparation.
Article
Chemistry, Analytical
Yuying Wang, Xuehua Wang, Qiuyue Sun, Ruijun Li, Yibing Ji
Summary: COFs, a type of crystalline polymers with controllable geometry and functionality, have attracted increasing interest. A novel chiral COF, β-CD COFBPDA, constructed through specific imine condensation, shows potential in separation science. Under optimized conditions, the COF-modified stationary phase demonstrates superior separation performance for racemic drugs.
Review
Pharmacology & Pharmacy
Xinyu Li, Qianjie Ma, Xiangtai Zheng, Qin Chen, Xiaodong Sun
Summary: Capillary electrochromatography (CEC) has a significant role in chiral separation, and open tubular capillary electrochromatography (OT-CEC) shows promising applications. OT-CEC stationary phases (SPs) developed over the past four years can be categorized into six types, and their characteristics in chiral drug separation are introduced. The applications of OT-CEC in metabolomics, food, cosmetics, environment, and biology, as well as its integration with other instruments like mass spectrometry (MS) and ultraviolet light detector (UV), are also discussed.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Pandeng Miao, Ying Xi, Zijie Feng, Jian Zhang, Yingxiang Du, Cheng Chen
Summary: The functionalized gold nanoparticle-based capillary column showed excellent chiral separation performance in separating drug enantiomers, with lactobionic acid as a chiral selector.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Genlin Sun, Weiyang Tang, Yao Lu, Kyung Ho Row
Summary: Biphasic chiral recognition was successfully applied for enantioseparation of acidic drugs in capillary electrochromatography, achieving outstanding separation efficiency and stability. The biphasic recognition system composed of beta-cyclodextrin additive and monolithic chiral stationary phase showed promising prospects for efficient enantioseparation of chiral compounds.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Wenqi Sun, Yikun Liu, Wei Zhou, Zhentao Li, Zilin Chen
Summary: The COF-V based CEC column demonstrates strong electroosmotic flow, high separation efficiency, and stability. It exhibits powerful separation selectivity to various compounds and excellent repeatability, making it suitable for complex sample analysis.
Article
Pharmacology & Pharmacy
Ning He, Zhentao Li, Changjun Hu, Zilin Chen
Summary: Covalent organic frameworks (COFs) are a new type of crystalline porous organic polymer materials with high thermal stability, porosity, structural regularity, and large specific surface area. In this study, a novel spherical COF TAPB-BPTA modified capillary column was developed as an electrochromatographic stationary phase, which demonstrated outstanding separation performance towards alkylbenzenes, phenols, and chlorobenzenes compounds. Additionally, baseline separations of non-steroidal anti-inflammatory drugs (NSAIDs) and parabens with good efficiency and high resolution were achieved. The results indicate that the COF-based stationary phase has tremendous application potential in the chromatographic separation field.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Xiangtai Zheng, Guihua Wu, Zhihao Yang, Na Guo, Bing Niu, Qin Chen, Xiaodong Sun
Summary: A novel chiral group functionalized metal-organic framework, Cyclodextrin-NH-MIL-53, was synthesized and utilized as a chiral capillary stationary phase for enantioseparation of racemic amino acids. Excellent separation of five pairs of enantiomers was achieved in this system. The prepared Cyclodextrin-NH-MIL-53 and capillary columns were characterized and the electrochromatography conditions were optimized. This research provides a novel insight and method for the design and use of metal-organic framework-based capillaries for enantioseparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Chen Yuan, Zhen Wang, Wanqi Xiong, Zhifeng Huang, Yalin Lai, Shiguo Fu, Jinqiao Dong, Aihong Duan, Xiandeng Hou, Li-Ming Yuan, Yong Cui
Summary: The use of high-performance chromatography technologies for separating enantiomers is of great importance and interest. β-cyclodextrin (β-CD) and its derivatives have been extensively studied as chiral stationary phases (CSPs), but their low stabilities and limited operating ranges have been a challenge. Covalent organic frameworks (COFs) with highly ordered nanopores have emerged as promising CSPs, but their practical applications have been hindered by difficulties in monomer and COF synthesis. This work introduces β-CD-modified COFs as potential CSPs for extensive enantioseparation in both gas chromatography and high-performance liquid chromatography, offering comparable resolution capability and superior application versatility compared to commercial chiral columns. By integrating the advantages and overcoming the defects of COFs and β-CD, this study advances COFs as platforms for chiral selector modification and shows great promise for practical chromatographic enantioseparation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
Changjun Hu, Zhentao Li, Zhuang Hu, Qiaoyan Li, Yuanyuan Fu, Zilin Chen
Summary: In this study, a novel covalent organic nanospheres material was designed and synthesized, and the introduction of crown ether building ligands resulted in excellent separation selectivity for compounds containing imidazole structure.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Genlin Sun, Weiyang Tang, Yao Lu, Kyung Ho Row
Summary: A high separation efficiency OT-CEC column was prepared for peptides separation by systematically evaluating key parameters and verifying the effectiveness and repeatability of the column. The results indicate that the open tubular capillary column modified with a two-layer copolymer shows great potential for protein separation in capillary electrochromatography.
Article
Nanoscience & Nanotechnology
Amit Nain, Shih-Chun Wei, Yu-Feng Lin, Yu-Ting Tseng, Ranju Prasad Mandal, Yu-Fen Huang, Chih-Ching Huang, Fan-Gang Tseng, Huan-Tsung Chang
Summary: BSA-encapsulated copper sulfide nanocrystals (CuS NCs) were prepared and exhibited enzyme-like and photodynamic activities. under NIR laser irradiation, the antimicrobial response was significantly enhanced and >99% of bacteria were eradicated in just 1 min. The BSA-CuS NCs showed insignificant cytotoxicity and hemolysis, making them highly biocompatible bactericides for effective bacteria eradication in preclinical applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Wei-Jen Chan, Fan-Gang Tseng
Editorial Material
Chemistry, Analytical
Tuhin Subhra Santra, Fan-Gang Tseng
Review
Chemistry, Analytical
Kiran Kaladharan, Ashish Kumar, Pallavi Gupta, Kavitha Illath, Tuhin Subhra Santra, Fan-Gang Tseng
Summary: The efficient delivery of foreign molecules into single living cells is crucial for cell biology, with single-cell measurement technologies offering a better understanding of molecular interactions. Various microfluidic-based physical methods play a key role in achieving single-cell transfection, aiding in the development of therapeutics and diagnostic tools.
Article
Nanoscience & Nanotechnology
Chao-Chu Wang, Monika Kataria, Hung- Lin, Amit Nain, Hsia Yu Lin, Christy Roshini Paul Inbaraj, Yu-Ming Liao, Anjali Thakran, Huan-Tsung Chang, Fan-Gang Tseng, Ya-Ping Hsieh, Yang-Fang Chen
Summary: Integrating hyperbolic metamaterials with highly luminescent silver metal nanoclusters has enabled strong stimulated emission, enhancing light emission by about 35 times compared to integration with a silicon substrate. This approach provides a plausible method for achieving stimulated laser action from metal nanoclusters.
Article
Nanoscience & Nanotechnology
Venkanagouda S. Goudar, Manohar Prasad Koduri, Yen-Nhi Ngoc Ta, Yunching Chen, Li-An Chu, Long-Sheng Lu, Fan-Gang Tseng
Summary: Three-dimensional spheroid culture provides a closer model of tumor growth in its natural context with the presence of the collagen network still unclear. A 3D co-culture model was developed to mimic the tumor microenvironment, showing increased drug resistance in tumor spheroids compared to 2D culture. Targeting collagen homeostasis can sensitize the spheroids to chemotherapy drugs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Kuan-Hung Chen, Shih-Han Lee, Li-Ching Kok, Tseren-Onolt Ishdorj, Hwan-You Chang, Fan-Gang Tseng
Summary: The study introduces a novel multi-functional microfluidic system, 3D-ACEK/SERS, which can rapidly concentrate bacteria from whole blood, identify bacterial species, and determine antibiotic susceptibilities. This system utilizes a hybrid electrokinetic mechanism to achieve high concentration efficiency and quick detection, with potential clinical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medicine, General & Internal
Yun-Jie Hao, Chih-Yung Yang, Ming-Hsien Chen, Lu-Wey Chang, Chien-Ping Lin, Liang-Chuan Lo, Sheng-Chieh Huang, You-You Lyu, Jeng-Kai Jiang, Fan-Gang Tseng
Summary: Insufficient prognosis of local recurrence in colorectal cancer (CRC) patients contributes to poor progression-free survival rate and death. This study investigated the potential of plasma/exosomal microRNA-21 (miR-21) expressions in predicting CRC recurrence and the possibility of improving prognostic efficacy in early CRC recurrence by combining biomarkers. The results showed significant correlations between plasma and exosomal miR-21 expressions, as well as similar correlations with other biomarkers. Different values of predicting CRC recurrence were observed in stratified patients based on high levels of exosomal miR-21 and plasma miR-21. Furthermore, the use of circulating tumour cells/microemboli (CTCs/CTM) as sensitive biomarkers showed comparable or even better performances in predicting CRC recurrence in late-stage patients. Therefore, analyzing combined biomarkers could improve the efficacy of prognosing CRC recurrence and differentiate recurrence in stratified patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Aum Sagar Panda, Yi-Chien Lee, Chen-Jung Hung, Kang-Ping Liu, Cheng-Yen Chang, Gkreti-Maria Manesi, Apostolos Avgeropoulos, Fan-Gang Tseng, Fu-Rong Chen, Rong-Ming Ho
Summary: This study demonstrates a facile method for the controlled orientation of nanostructures in thin films using a diblock copolymer system. By manipulating the pressure and surface tension of the polymer materials, the researchers were able to achieve long-range order in the nanostructures.
Article
Chemistry, Analytical
Kirsty Muldoon, Zeeshan Ahmad, Yu-Chuan Su, Fan-Gang Tseng, Xing Chen, James A. D. McLaughlin, Ming-Wei Chang
Summary: Personalised drug delivery systems with real-time imaging have the potential to revolutionize diagnostic and therapeutic applications. In this study, a 3D-printed drug delivery matrix with real-time imaging capability was developed, offering promising applications in drug delivery systems, medical imaging, and targeted therapy.
Editorial Material
Cell Biology
Tuhin Subhra Santra, Fan-Gang Tseng
Article
Biotechnology & Applied Microbiology
Wei-Jen Chan, Sasinan Bupphathong, Han-Lin Cho, Venkanagouda S. Goudar, Sina Dehestani, Chi-Shiun Chiang, Fan-Gang Tseng
Summary: Poly(vinyl alcohol)/boric acid crosslinked nanoparticles (PVA/BA NPs) demonstrated effective therapeutic results for oral cancer treatments in BNCT, with a significant reduction in tumor size compared with clinically used boronophenylalanine in a murine model.
Article
Polymer Science
Fu-Rong Chen, Rong-Ming Ho, Chen-Jung Hung, Aum Sagar Panda, Yi-Chien Lee, Shih-Yi Liu, Jheng-Wei Lin, Hsiao-Fang Wang, Apostolos Avgeropoulos, Fan-Gang Tseng
Summary: In this study, the controlled self-assembly of star-block polystyrene-blockpolydimethylsiloxane (PS-b-PDMS) thin films was directly visualized using in situ transmission electron microscopy (TEM) observations. The self-alignment process of film-spanning perpendicular cylinders in the block copolymer (BCP) thin films was investigated under low-dose conditions using an environmental chip with a built-in microheater. The time-resolved self-alignment process was systematically compared between symmetric and asymmetric conditions, providing comprehensive insights into the nucleation and growth mechanism.
Article
Chemistry, Analytical
Fan-Gang Tseng, Wei-Cheng Chiu, Po-Jung Huang
Summary: This paper proposes a Swiss-roll-type mini-reformer employing a copper-zinc catalyst for high-efficient SRM process. The Swiss-roll-shaped design improves thermal energy preservation and temperature uniformity, resulting in higher conversion rates and better long-term durability compared to other geometries.
Article
Chemistry, Analytical
Ashish Kumar, Venkanagouda S. Goudar, Kiran Kaladharan, Tuhin Subhra Santra, Fan-Gang Tseng
Summary: Temperature dynamically regulates the reactivity of biomolecules in the cellular environment, including solid tumors. This study utilized fluorescent polymeric nano-thermometers (FPNTs) to measure the intratumor temperature in co-cultured 3D tumor spheroids. The results showed that FPNTs have great stability, biocompatibility, and high intensity in a biological medium. They can be used as a multifunctional adjuvant to examine thermoregulation in tumor spheroids and provide physiologically relevant spatio-temporal information about solid tumors.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)