Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 28, Issue 12, Pages 1744-1755Publisher
WILEY
DOI: 10.1002/bmc.3216
Keywords
UPLC; Q-TOF-MS; glycerophospholipid; RAW264; 7 cell; identification; fragmentation regularity
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Funding
- National Science Foundations of China [21275036, 81202429]
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The study aims to develop a rapid, sensitive ultra-performance liquid chromatography coupled with an electrospray ionization quadruple time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) analytical method for identifying glycerophospholipids (GPLs) from RAW264.7 cells. A total of 78 GPLs including 22 phosphatidylethanolamines (PEs), 49 phosphatidylcholines (PCs), four phosphatidylglycerols, one phosphatidylinositol and two unknown GPLs were identified. PC (14:0/16:1), PC (14:0/16:0), PE (0:0/20:3), PE (22:5/0:0) and PE (22:3/0:0) were identified for the first time. The UPLC/Q-TOF-MS method is suitable for targeting analysis of GPLs from RAW264.7 cells, which allows us to find out new GPLs compositions related to inflammatory diseases and to explain their pharmacological roles in inflammatory process. Copyright (c) 2014 John Wiley & Sons, Ltd.
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