Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 27, Issue 9, Pages 1162-1167Publisher
WILEY
DOI: 10.1002/bmc.2922
Keywords
oleuropein; in vivo metabolism; feces; urine; LC-MS/MS
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Funding
- Ministry of Science and Technology of the People's Republic of China (National S&T Major Project) [2012ZX09301002-001]
- National Natural Science Foundation of China [81073137]
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A highly sensitive and specific LC-MS/MS method was developed to investigate the in vivo bio-transformation of oleuropein in rat. Rat feces and urine samples collected after oral administration were determined by liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative-ion mode. The assay procedure involves a simple liquid-liquid extraction of parent oleuropein and the metabolite from rat feces and urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.50 mL/min on an RP-C-18 column with a total run time of 31 min. This method was successfully applied to simultaneous determination of oleuropein and its metabolites in rat feces and urine. De-glucosylation, hydrolysis, oxygenation and methylation were found to comprise the major metabolic pathway of oleuropein in rat gastrointestinal tract and three metabolites were absorbed into the blood circulatory system within 24 h after oral administration. Copyright (C) 2013 John Wiley & Sons, Ltd.
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