Review
Chemistry, Medicinal
Sharuti Mehta, Anil Kumar Sharma, Rajesh K. Singh
Summary: Picrorhiza kurroa is a well-recognized Ayurvedic herb with iridoid glycosides as its bioactive constituents, exhibiting various pharmacological activities. However, due to indiscriminate exploitation, ecological destruction, slow plant growth, and lack of awareness regarding cultivation and uprooting, it has become endangered.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Ashish Sharma, Dipto Bhattacharyya, Shilpa Sharma, Rajinder Singh Chauhan
Summary: The study investigates the biosynthetic machinery of picrosides in Picrorhiza kurroa, showing that the regulation and degradation of ABA play crucial roles in the organ-specific accumulation of picroside-I and picroside-II. Biosynthesis, condensation and glucosylation of isoprene units may occur in shoots, roots or stolons.
Article
Plant Sciences
Roma Pandey, Ashish Sharma, Hemant Sood, Rajinder Singh Chauhan
Summary: This study reveals potential clues on ABC transporters involved in long distance and local transport of picrosides in P. kurroa organs, and further unravels important interactions with additional components of biosynthetic machinery through co-expression network analysis.
Article
Biochemistry & Molecular Biology
Qiuchan Huan, Zhengxian Gao, Peigen Wu, Yingdi Zhang, Haitao Xiao, Jiao Peng
Summary: This study investigates the protective effects of Picroside III on the intestinal epithelial barrier in TNF-alpha induced Caco-2 cells and DSS induced colitis in mice. The results demonstrate that Picroside III alleviates colitis symptoms and promotes wound healing and barrier function recovery. Mechanism studies indicate that the activation of AMPK is involved in the protective effects of Picroside III.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Pharmacology & Pharmacy
Yusuke Sakamoto, Naoki Inoue, Yusuke Nakanishi, Kiyofumi Ninomiya, Masayuki Yoshikawa, Osamu Muraoka, Yoshiaki Manse, Toshio Morikawa
Summary: A methanol extract of Picrorhiza kurroa Royle ex Benth. rhizomes showed hepatoprotective effects against D-GalN/LPS-induced liver injury in mice. Picroside II, androsin, and 4-hydroxy-3-methoxyacetophenone exhibited active hepatoprotective effects. These compounds decreased D-GalN-induced cytotoxicity, without inhibiting LPS-induced macrophage activation, and reduced the sensitivity of hepatocytes to TNF-a.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2023)
Article
Biochemistry & Molecular Biology
PeiLiang Dong, ZhenLei Gao, Xin Yin, ZhengQing Li, Hua Han
Summary: Picrorhiza kurroa, a traditional medicinal plant in China and India, is commonly used for treating liver diseases and other ailments. Studies have shown that its main constituents, picroside I and II, have anti-inflammatory and hepatoprotective activities. By synthesizing derivatives from these compounds, researchers found that some derivatives demonstrated improved hepatoprotective effects in vitro.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Biochemical Research Methods
Shweta Thakur, Jyoti Chhimwal, Robin Joshi, Manglesh Kumari, Yogendra Padwad, Rajiv Kumar
Summary: A novel bioactive peptide BP1 from Picrorhiza kurroa Royle ex Benth. was found to have antioxidant potential and inhibitory activities against ACE and DPP-IV, making it a potential candidate for peptide-based therapeutics in treating diabetes, cardiovascular diseases, and other diseases associated with ROS.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Anju Thapa, Ravinder Kaushik, Smriti Arora, Sundeep Jaglan, Varun Jaswal, Virendra Kumar Yadav, Manjeet Singh, Aarti Bains, Prince Chawla, Azhar Khan, Melinda Fogarasi, Szabolcs Fogarasi
Summary: Yersiniosis, a zoonotic disease caused by Yersinia enterocolitica, is prevalent in Europe and known for its high antibiotic resistance. This study evaluated the antimicrobial activity of an extract from Picrorhiza kurroa against Yersinia enterocolitica. The extract showed effective antimicrobial activity and potential bioactive compounds were identified. Picroside-1 exhibited higher antimicrobial potential and lysed Yersinia enterocolitica cells. Docking analysis revealed its interaction with the dihydrofolate reductase protein, suggesting it as a possible drug candidate for Yersinia enterocolitica.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Vijay Laxmi Trivedi, Sudeep Chandra, M. C. Nautiyal
Summary: The study evaluated the impact of P. polystachyum on the morpho-physiology and biochemistry of Picrorhiza kurroa in the Western Himalayan alpine regions. It was found that Picrorhiza kurroa plants growing with P. polystachyum showed better growth and physiological characteristics, demonstrating greater adaptability.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Plant Sciences
Manglesh Kumari, Upendra Kumar Pradhan, Robin Joshi, Ashwani Punia, Ravi Shankar, Rajiv Kumar
Summary: Picrorhiza kurroa, a high altitude medicinal herb of Himalaya, is rich in phytochemicals with medicinal potential attributed to picrosides. A comprehensive proteome reference map was established, revealing protein abundance across different organs and developmental stages. The study highlights the roles of stress-responsive proteins and post-translational modifications in P. kurroa adaptation to alpine environment, providing insights for future research on stress tolerance and medical applications.
Review
Biotechnology & Applied Microbiology
Tanvi Sharma, Upendra Sharma, Sanjay Kumar
Summary: Iridoid glycosides in the genus Picrorhiza play important roles in pharmaceutical research and are used as bioactive ingredients in drug formulations. However, due to factors such as unregulated harvesting and limited cultivation, these plant species are endangered. Biotechnological and molecular interventions are needed to address these challenges and facilitate large-scale propagation and genetic manipulation.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhibin Wang, Xuepeng Shi, Shuang Jiang, Jiahui Sun, Gilwa Borjigin, Qi Li, Yuanqiu Mu, Chunjuan Yang, Zhenyue Wang, Haixue Kuang
Summary: In this study, a UHPLC-ESI-MS/MS method was developed to simultaneously determine multiple compounds in rat plasma. The method showed good linearity and low limits of quantification, and provided insights into the behavior of drugs in the body and the body's effect on drugs.
Review
Biochemistry & Molecular Biology
Tahani M. Almeleebia, Abdulrhman Alsayari, Shadma Wahab
Summary: Traditional medicinal herb Picrorhiza kurroa has been widely used for the treatment of various ailments due to its promising pharmacological potential. This study comprehensively evaluated the efficacy, phytochemistry, pharmacological activity, dose, and toxicity of P. kurroa, providing comprehensive insights for future research and therapeutic applications. P. kurroa may serve as a natural alternative to standard treatments for emerging diseases, making this study a valuable resource for fundamental and clinical investigations.
Article
Biochemistry & Molecular Biology
Anjali Kharb, Shilpa Sharma, Ashish Sharma, Neeti Nirwal, Roma Pandey, Dipto Bhattacharyya, Rajinder Singh Chauhan
Summary: By analyzing the ATs sequences in different organs from tissue culture and nursery-grown plants, we identified six potential ATs involved in P-II formation. Through gene expression validation and protein-ligand docking analysis, we confirmed that one of these ATs may be a key component in the final structure of P-II.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Chemistry, Medicinal
Peigen Wu, Churui Chang, Guanglin Zhu, Lixiang Zhai, Xu Zhang, Qiuchan Huan, Zhengxian Gao, Huan Deng, Yue Liang, Haitao Xiao
Summary: This study explored the potential mechanism of glycosidic fraction of Picrorhiza scrophulariiflora Pennell (GPS) extract for the treatment of colitis using UPLC-QTOF-MS analysis, network pharmacology and experimental research. The results showed that GPS extract exerts its anti-inflammatory effect against colitis by suppressing the PI3K/Akt and MAPK pathways.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2023)