Review
Biochemistry & Molecular Biology
Nian Shi, Xinmiao Bu, Manyu Zhang, Bin Wang, Xinli Xu, Xuezhong Shi, Dilshad Hussain, Xia Xu, Di Chen
Summary: This review summarizes the recent progress and advances in sample preparation techniques combined with separation-based detection methods (such as capillary electrophoresis or liquid chromatography with various detectors) for the determination of catecholamine neurotransmitters in biological samples from 2015 to 2021. Due to the low concentrations of Catecholamines (CAs) and the interference from co-existing biological compounds, direct analysis often faces severe difficulties, making sample preparation techniques crucial for accurate quantification. The review provides guidance and assistance for researchers interested in diagnostic analysis and biological systems to choose suitable sample pretreatment and detection methods.
Article
Biochemistry & Molecular Biology
Maria Zorina, Victor V. Dotsenko, Pavel N. Nesterenko, Azamat Temerdashev, Ekaterina Dmitrieva, Yu-Qi Feng, Sanka N. Atapattu
Summary: In this study, phthalylglycyl chloride was proposed as a derivatization agent for the determination of octopamine, adrenaline, and dopamine in urine for the first time. The derivatization process combined reversed-phase liquid chromatography with positive electrospray ionization-high-resolution mass spectrometry. An LC-HRMS method with quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes was developed. The derivatization with phthalylglycyl chloride at pH 6.5 and 30 degrees C resulted in a 95-97% yield of derivatives. The proposed method was successfully applied to the analysis of human urine samples and compared with conventional derivatization procedures using 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
Article
Biochemical Research Methods
Apostolia Tsiasioti, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: In this article, a new approach to reduce the consumption of reagents in liquid chromatography coupled to online post column derivatization is proposed. The proposed method, called Pulsed-Post Column Derivatization (Pulsed-PCD), introduces reagents as well-defined pulses at microliter levels. The robustness of the procedure was evaluated and the method was successfully applied to determine histidine in human urine.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Yanping Lin, Krista Spiller, Radha Aras, Wenying Jian
Summary: A highly selective and sensitive UPLC-MS/MS assay was developed for quantitation of six catecholamines and their metabolites in rat and mouse striatum as pharmacodynamic biomarkers to support neuroscience and pharmaceutical research. The assay successfully measured the six analytes in striatum from mice treated with the neurotoxin MPTP and confirmed the metabolic pathway of neurotoxicity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Hao Feng, Fan Wang, Junhua Li, Qian Wu, Ying Cui, Lingzhi He, Xing Liu, Zeng Liu, Dong Qian, Haixia Tong
Summary: This study successfully synthesized four types of Fe-Co bimetallic Prussian blue analogues with different ratios of Fe to Co. The optimized FeCo-PBA showed excellent performance as an electrocatalyst for 5-HT sensing, with high sensitivity and low detection limit.
Article
Chemistry, Analytical
Yuanyuan Xia, Yuan Wang, Meng Zhang, Faqiong Zhao, Baizhao Zeng
Summary: A novel composite material with good conductivity and strong adsorption capacity for 5-HT was synthesized, showing wide linear response range and low detection limit in electrochemical determination. The sensor exhibited high selectivity, stability, and reproducibility, and was successfully applied to the detection of 5-HT in human serum and urine samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Youxi Jin, Yu Pan, Biao Jin, Dongri Jin, Chunbo Zhang
Summary: The chiral derivatization reagent Pro-PPZ was effective for enantioseparation and ultra-sensitive detection of chiral amines. The method was successfully applied in metabolite analysis, showing that R-AI was not converted into S-AI during metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Jing Chen, Jiao Yang, Ying Chen, Jianbin Dong, Rong Deng, Lianqin Zhu
Summary: In this study, a oxidation-reduction active three-dimensional metal-organic framework (MOF) was successfully synthesized and used to prepare an electrochemical sensor for detecting 5-HT, showing promising practical application value.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: In this study, naphthalene-2,3-dicarboxaldehyde (NDA) was used in Pulsed-PCD, a new concept for on-line post column derivatization (PCD) coupled with liquid chromatography. The use of expensive reagents in PCD was minimized by introducing the reagents into the mobile phase flow at precise timing. NDA-CN chemistry was applied to determine histamine in food samples, while two additional methods based on histamine reactions with o-phthalaldehyde (OPA) were developed. The consumption of PCD reagents was significantly reduced by using a 50 μl pulse (OPA/NDA + Buffer) in all three methods.
Article
Chemistry, Analytical
Elizabeth L. Rigby, Rachel A. Saylor
Summary: Dopamine and serotonin are neurotransmitters that play important roles in brain function and diseases. By investigating the fluorescence properties of certain compounds, valuable insights can be gained for neuroscientific research.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Eirini Stampina, Apostolia Tsiasioti, Kalliopi Klimatsaki, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: A reliable and highly selective method for the determination of histidine in human serum and urine was developed, utilizing cation exchange chromatography and online post column derivatization, which showed good linearity, low detection limit, and minimal matrix effect. The determination of endogenous histidine in human samples demonstrated satisfactory percent recoveries, ranging between 89 and 114%.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Taelor M. Zarkovic, Scott A. Borden, Erik T. Krogh, Chris G. Gill
Summary: To improve the ionization sensitivity and selectivity in liquid chromatography-mass spectrometry, chemical modifiers are commonly added post-separation. This study introduces a passive semi-permeable hollow fiber membrane approach for the addition of chemical modifiers, which overcomes the issues of sample dilution and imperfect mixing.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Emmanuel O. Ogunkunle, Joshua J. Davis, Emily L. Skinner, James Thornham, Michael G. Roper
Summary: This study evaluated the content and release of D-amino acids in islet secretions and confirmed an effective separation method. The results showed that D-serine and D-glutamine were secreted at low concentrations with no significant difference between glucose concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
E. V. Dmitrieva, A. Z. Temerdashev, M. O. Zorina, Yu-Qi Feng, P. N. Nesterenko
Summary: The possibility of applying solid-phase analytical derivatization (SPAD) for quantifying seven steroid hormones in human urine was investigated. The proposed method involved enzymatic hydrolysis, followed by SPAD with hydroxylamine. The method was optimized to achieve high recovery values and demonstrated applicability for routine analysis of real urine samples using reversed-phase ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Justyna Piechocka, Natalia Litwicka, Rafal Glowacki
Summary: This study provides evidence for the formation of the adduct 1,3-thiazinane-4-carboxylic acid (TCA) in humans through the reaction of homocysteine/thiolactone (Hcy/HTL) and formaldehyde (FA). A GC-MS method has been developed and successfully applied for the quantification of TCA in human urine samples. This method may serve as a new analytical tool for the investigation of TCA's role in living systems in routine clinical analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)