☆ 4.4 Article

A simple and cost effective method for the quantification of 8-hydroxy-2′-deoxyguanosine from urine using liquid chromatography tandem mass spectrometry

BIOMEDICAL CHROMATOGRAPHY (2008)

Journal

BIOMEDICAL CHROMATOGRAPHY

Volume 22, Issue 4, Pages 394-401

Publisher

JOHN WILEY & SONS LTD

DOI: 10.1002/bmc.946

Keywords

8-hydroxy-2'-deoxyguanosine; DNA biomarkers; oxidative stress; LC-MS/MS

Categories

Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy

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Abstract

A rapid and high-throughput method for the determination of urinary levels of the oxidative stress biomarker, 8-hydroxy-2'-deoxyguanosine (8-OH-dG), has been developed and validated using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-MS/MS). The assay features a cheap and readily available non-isotopic internal standard, a single-step filtration sample preparation, and a total analysis time of 6 min including column re-equilibration. The method was validated based on linearity, accuracy (100-106%), precision (CV < 7%), sample preparation stability (<= 5%, 72 h). Intra-laboratory patient ranges were established comparing children and adults (n = 345). Copyright (c) 2007 John Wiley & Sons, Ltd.

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