Journal
ANALYTICA CHIMICA ACTA
Volume 889, Issue -, Pages 106-112Publisher
ELSEVIER
DOI: 10.1016/j.aca.2015.06.052
Keywords
Aptasensor; Cocoa beans; Label free; Impedimetric; Ochratoxin; Screen printed electrodes
Categories
Funding
- European project COCOtA: Ministere de l'agriculture, de l'agroalimentaire et de la foret
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Contamination of food by mycotoxin occurs in minute/trace quantities. Nearly 92.5% of the cocoa samples present Ochratoxin A (OTA) levels at trace quantity. Hence, there is a necessity for a highly sensitive and selective device that can detect and quantify these organic toxins in various matrices such as cocoa beans. This work reports for the first time, a facile and label-free electrochemical impedimetric aptasensor for rapid detection and quantitation of OTA in cocoa beans. The developed aptasensor was constructed based on the diazonium-coupling reaction mechanism for the immobilization of anti-OTA-aptamer on screen printed carbon electrodes (SPCEs). The aptasensor exhibited a very good limit of detection (LOD) as low as 0.15 ng/mL, with added advantages of good selectivity and reproducibility. The increase in electron transfer resistance was linearly proportional to the OTA concentration in the range 0.15-2.5 ng/mL, with an acceptable recovery percentage (91-95%, RSD = 4.8%) obtained in cocoa samples. This work can facilitate a general model for the detection of OTA in cocoa beans based on the impedimetric aptasensor. The analysis can be performed onsite with pre-constructed and aptamer modified electrodes employing a portable EIS set up. (C) 2015 Elsevier B.V. All rights reserved.
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