Article
Orthopedics
Reza Kakavand, Akbar Rasoulian, Baaba S. Otoo, Walter Herzog, Amin Komeili
Summary: This study developed a collagen fibril remodeling algorithm to describe the unique organization of collagen fibrils in a 3D knee model. The findings revealed that collagen fibrils in articular cartilage exhibit distinct orientation patterns, indicating the involvement of mechanobiological processes in the maturation of newborn collagen fibrils.
Article
Materials Science, Multidisciplinary
Lilan Gao, Luming Fenga, Yansong Tan, Qijun Gao, Gang Liu, Chunqiu Zhang
Summary: This study investigates the behavior of knee articular cartilage under biaxial cyclic loading and analyzes the associated microstructural changes. The results show that constraints have a clear influence on strain evolution and mean strain, and the loading path and stress amplitude also impact mean strain. Additionally, the rearrangement of collagen fibril networks is closely related to the stress state of cartilage.
MECHANICS OF MATERIALS
(2023)
Article
Biophysics
Mikko T. Nissinen, Nina Hanninen, Mithilesh Prakash, Janne T. A. Makela, Mikko J. Nissi, Juha Toyras, Miika T. Nieminen, Rami K. Korhonen, Petri Tanska
Summary: The study investigated the changes in fibril-reinforced poroelastic material parameters of human patellar cartilage at different stages of osteoarthritis. It found significant differences in these parameters and their associations with proteoglycan content, collagen fibril orientation angle, and optical retardation. Additionally, reductions in the initial fibril network modulus and permeability were observed in advanced osteoarthritis samples, showing correlations with structural and functional changes in the cartilage.
JOURNAL OF BIOMECHANICS
(2021)
Article
Engineering, Biomedical
Anna Puiggali-Jou, Riccardo Rizzo, Angela Bonato, Philipp Fisch, Simone Ponta, Daniel M. Weber, Marcy Zenobi-Wong
Summary: Tissue engineering methods have potential for restoring cartilage function, but reproducing cartilage zonal architecture is still a challenge. This study demonstrates that Filamented Light biofabrication can significantly improve cartilage tissue maturity by generating constructs with zonal architecture and native-like mechanical properties.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Anatomy & Morphology
Mario Raspanti, Marina Protasoni, Piero Antonio Zecca, Marcella Reguzzoni
Summary: The free surface of articular cartilage is crucial for the tissue's physiology, containing a thin superficial layer rich in glycoconjugates and a thicker layer of collagen fibrils beneath to redistribute stress.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Orthopedics
Emma N. Luke, Pahweenvaj Ratnatilaka Na Bhuket, Seungju M. Yu, Jeffrey A. Weiss
Summary: The objective of this study was to investigate the potential of collagen hybridizing peptides (CHPs) to extend the retention time of near-infrared fluorophores (NIRF) following intra-articular injection in rat knee joints. The study found that CHPs were retained within the joint for the full study period and significantly extended the retention time of NIRFs by binding to multiple collagenous tissues in the joint. These results support further research to develop CHP based therapeutics for the treatment of osteoarthritis.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Materials Science, Biomaterials
Shalini Sundar, Renata Linardi, Angela Gaesser, Tianzheng Guo, Kyla Ortved, Julie Engiles, Justin Parreno, Charles Dhong
Summary: Articular cartilage derives its load-bearing strength from the coupling between the collagen network and proteoglycans. We developed a platform using graphene strain sensors to measure tissue swelling in horse cartilage. Our platform can decouple the contributions of proteoglycan degradation and collagen degradation, and analyze multiple samples simultaneously.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biophysics
Sana Jahangir, Amir Esrafilian, Mohammadhossein Ebrahimi, Lauri Stenroth, Tine Alkjaer, Marius Henriksen, Martin Englund, Mika E. Mononen, Rami K. Korhonen, Petri Tanska
Summary: In this study, the mechanical responses of knee cartilage were investigated using a finite element knee joint model with either human or bovine fibril-reinforced poroviscoelastic cartilage properties during walking. The results showed that bovine cartilage properties led to greater stresses and fluid pressures compared to human cartilage properties. The mechanical responses of cartilage were highly sensitive to variations in collagen fibril-related material parameters.
JOURNAL OF BIOMECHANICS
(2023)
Review
Biochemistry & Molecular Biology
Darina Bacenkova, Marianna Trebunova, Jana Demeterova, Jozef Zivcak
Summary: Hyaline cartilage lacks self-repair potential, but tissue engineering methods can be used to address the issue of cartilage defect repair. Research shows that chondrocytes and mesenchymal stromal cells have favorable repair abilities in a three-dimensional culture environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
A. C. Moore, M. G. Hennessy, L. P. Nogueira, S. J. Franks, M. Taffetani, H. Seong, Y. K. Kang, W. S. Tan, G. Miklosic, R. El Laham, K. Zhou, L. Zharova, J. R. King, B. Wagner, H. J. Haugen, A. Munch, M. M. Stevens
Summary: This paper reports on the development of an engineered poroelastic material that begins to approach physiological performance. The material achieved a mean peak fluid load fraction of 68%, displayed consistency with mixture theory, and demonstrated cytocompatibility. This work creates a foundation for designing poroelastic cartilage implants and developing scaffold systems to study chondrocyte mechanobiology and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Ming Zhang, Chenglei Liu, Huimin Lin, Hanqi Wang, Le Qin, Zhiyong Zhang, Chunlei Liu, Yong Lu, Fuhua Yan, Yuyao Zhang, Hongjiang Wei
Summary: This study quantified the age-related differences in cartilage susceptibility using QSM in a healthy population and found that the susceptibility increased with age in the deeper regions of knee cartilage. The findings suggest that QSM has the potential to reveal age-related changes in cartilage structure.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Article
Biotechnology & Applied Microbiology
Xiang Li, Shiao Li, Jiatian Qian, Yancheng Chen, Yiqin Zhou, Peiliang Fu
Summary: The study demonstrated that type I collagen-based MACT is an effective treatment for improving functionality and pain levels. Histological evidence confirmed hyaline cartilage induction and showed that repaired cartilage tended to emerge from the deep to the superficial layer. The quantitative MRI T2 mapping test indicated that there still was a difference between the transplanted cartilage and the surrounding hyaline cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Katarzyna Styczynska-Soczka, Anish K. Amin, Andrew C. Hall
Summary: Abnormal chondrocytes are present in nondegenerate cartilage, suggesting dedifferentiation and production of a weakened matrix. With degeneration, the percentage of normal chondrocytes decreases while abnormal cells and clusters increase, along with collagen type I labelling. The study also found an increase in collagen type I around chondrocytes with degeneration, indicating fibro-cartilaginous repair tissue progression similar to osteoarthritis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ikram Mohout, Seyed Ali Elahi, Amir Esrafilian, Bryce A. A. Killen, Rami K. K. Korhonen, Sabine Verschueren, Ilse Jonkers
Summary: This study used a multi-scale in silico modeling approach to evaluate the mechanoregulatory factors indicative of collagen degradation and proteoglycans depletion in knee osteoarthritis (KOA). The results showed that these factors could distinguish between healthy individuals and KOA patients, and predict the risk of disease progression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Seyed Shayan Sajjadinia, Bruno Carpentieri, Gerhard A. Holzapfel
Summary: Classical continuum mechanics is commonly used for implementing material models of articular cartilage, but the initial stress-free configuration is contradictory to the pre-stressed state in experiments, leading to non-physiological expansion of the FE mesh. By developing an efficient algorithm to restore stress-free conditions and optimize variables, the study showed significant improvements in model fidelity and stress distribution accuracy.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Gustavo A. Orozco, Aapo Ristaniemi, Mehrnoush Haghighatnejad, Ali Mohammadi, Mikko A. J. Finnila, Simo Saarakkala, Walter Herzog, Hanna Isaksson, Rami K. Korhonen
Summary: The rupture of the anterior cruciate ligament (ACL) leads to alterations in the elastic and viscoelastic properties of the collateral ligaments in the knee joint. This adaptation may have important implications for the overall knee joint mechanics and provides insight into the consequences of ACL rupture on other tissues. The study characterizes the changes in the ligaments following ACL transection (ACLT) in rabbits and highlights the early adaptation of the collagen fibrillar network in the collateral ligaments.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Mechanical
Anna Gustafsson, Hanna Isaksson
Summary: In this study, a selection of recent open-source implementations of the phase field method was evaluated to find the best approach for simulating crack growth in bone tissue. A new method for correcting the interface fracture toughness in a phase field model was also proposed. The results showed that the quasi-Newton monolithic solvers outperformed the evaluated staggered solvers in terms of computational speed and robustness, and correcting the fracture toughness of the interface is crucial for simulating crack patterns.
THEORETICAL AND APPLIED FRACTURE MECHANICS
(2022)
Article
Biophysics
Thomas Notermans, Hanna Isaksson
Summary: This study explores the mechanobiological mechanisms underlying the formation of different tissue types during Achilles tendon healing. Using a computational model, the researchers predict the spatiotemporal formation of tendon, cartilage, bone, and fat-like tissue. The results suggest that mechanobiology plays a role in governing the formation of these tissues during tendon healing.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isabella Silva Barreto, Maria Pierantoni, Malin Hammerman, Elin Tornquist, Sophie Le Cann, Ana Diaz, Jonas Engqvist, Marianne Liebi, Pernilla Eliasson, Hanna Isaksson
Summary: The structural response of collagen fibrils in Achilles tendons during load to rupture, cyclic loading, and stress relaxation was investigated using in situ loading and small-angle X-ray scattering. The fibrils exhibited lower strain than the applied tissue strain, and showed linear strain in the elastic region of the tissue. During cyclic loading, the fibrils demonstrated increased stretchability and recovery, while during tissue stress relaxation, they showed relaxation of fibril strain. Fibril strain heterogeneity increased with applied loads and became increasingly disordered after the toe region.
Correction
Cell Biology
Ida Axelsson, Anna Gustafsson, Hanna Isaksson, Johan Nilsson, Torsten Malm
CELL AND TISSUE BANKING
(2023)
Article
Cell Biology
Ida Axelsson, Anna Gustafsson, Hanna Isaksson, Johan Nilsson, Torsten Malm
Summary: The optimal time spans for homograft procurement are still controversial and further research is needed. This study aimed to analyze the mechanical properties of homograft tissue at different time spans during the procurement process. Results showed that samples cryopreserved after 2-4 days had significantly higher elastic modulus and yield stress compared to those cryopreserved after 7-9 days. However, there was no difference in collagen and elastin content at different time spans. These findings suggest a potential deterioration of elastin and collagen after longer decontamination intervals, but their clinical significance remains uncertain.
CELL AND TISSUE BANKING
(2023)
Review
Biochemistry & Molecular Biology
Maria Pierantoni, Malin Hammerman, Isabella Silva Barreto, Daniel Larsson, Thomas Notermans, Andrew J. Bodey, Pernilla Eliasson, Hanna Isaksson
Summary: Achilles tendon rupture is a common medical condition that can result in functional loss. The healing process is slow and can be affected by heterotopic ossification (HO), which is the pathological formation of bone tissue instead of tendon tissue. This study used advanced imaging techniques to examine the temporal and spatial progression of HO during Achilles tendon healing in a rat model. The results revealed that HO deposition begins as early as one week after injury in the distal stump and mostly grows on preexisting HO deposits. These deposits become larger and calcified, occupying up to 10% of the tendon volume. The study provides valuable insights into the ossification process in healing tendons.
Correction
Biochemical Research Methods
Gustavo A. Orozco, Kalle Karjalainen, Eng Kuan Moo, Lauri Stenroth, Petri Tanska, Jaqueline Lourdes Rios, Teemu V. Tuomainen, Mikko J. Nissi, Hanna Isaksson, Walter Herzog, Rami K. Korhonen
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Maria Pierantoni, Isabella Silva Barreto, Malin Hammerman, Vladimir Novak, Ana Diaz, Jonas Engqvist, Pernilla Eliasson, Hanna Isaksson
Summary: This study investigates the effects of reduced loading on the structural properties and mechanical response of rat Achilles tendons. The results show that reduced loading leads to more crimped and less organized fibers, which could explain the altered mechanical response.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Hector Dejea, Deepak Bushan Raina, Isabella Silva Barreto, Kunal Sharma, Yang Liu, Dario Ferreira Sanchez, Ulf Johansson, Hanna Isaksson
Summary: This study aims to characterize bone mineralization using an in-vivo long bone fracture healing model in the rat. Multi-scale measurements indicated that zinc is required for both the initial mineral deposition and resorption processes, zinc and MMP-13 show similar spatio-temporal trends during early mineralization, and iron acts locally and in coordination with zinc during mineralization. These findings improve the understanding of bone mineralization.
ACTA BIOMATERIALIA
(2023)
Article
Endocrinology & Metabolism
Lorenzo Grassi, Sami P. P. Vaananen, Lars Jehpsson, Osten Ljunggren, Bjorn E. Rosengren, Magnus K. K. Karlsson, Hanna Isaksson
Summary: This study evaluated the ability of a method to predict incident hip fractures based on the reconstruction of 3D hip anatomy from a 2D DXA image and subject-specific FE-based prediction of proximal femoral strength. The results showed that FE-predicted proximal femoral strength was a better predictor of hip fractures than aBMD, suggesting potential for improving fracture risk predictions in a clinically feasible manner.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Carmel J. Caruana, Richard A. Amos, Diego Burgos, Stan Heukelom, Marija Z. Jeremic, Petro Julkunen, Violeta Karenauskaite, Loredana Marcu, Emmanouil Papanastasiou, Csilla Pesznyak
Summary: Although Medical Physics educators have historically contributed to the education of the non-physics healthcare professions, their role was not studied systematically until 2009 when EFOMP set up a group to research the issue. The group conducted a literature review, carried out a survey, and proposed a development model for the role based on the research findings. A comprehensive curriculum development model was subsequently published, and plans were made to develop a policy statement.
PHYSICA MEDICA-EUROPEAN JOURNAL OF MEDICAL PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Maria Pierantoni, Malin Hammerman, Isabella Silva Barreto, Linnea Andersson, Vladimir Novak, Hanna Isaksson, Pernilla Eliasson
Summary: Heterotopic mineralization is common in human tendons and is associated with tendinopathies, tendon weakness, and pain. In this study, heterotopic ossification (HO) was observed in intact rat Achilles tendons, with HO deposits characterized by an elongated shape and a fiber-like internal structure. Additionally, needle injury caused tissue microdamage and may be related to the deposition of HO. This research provides insights into the relationship between local inflammation, microdamage, and HO deposition.
JOURNAL OF STRUCTURAL BIOLOGY-X
(2023)
Article
Cell & Tissue Engineering
V. Sotiriou, Y. Huang, S. Ahmed, H. Isaksson, N. C. Nowlan
Summary: This study investigated the effects of muscle absence on skeletal growth and development in mouse embryos. The results showed that the detrimental effects of absent muscle on bones and joints became less severe as the embryos developed. The mineralisation extent showed the most pronounced changes over gestation. Understanding how bones and joints develop in the absence of muscle can provide insights into conditions such as hip dysplasia and arthrogryposis.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Neurosciences
Jonathan A. Williams, Carmen Huesa, Mikael J. Turunen, James A. Oo, Oskars Radzins, Wilf Gardner, James F. C. Windmill, Hanna Isaksson, K. Elizabeth Tanner, John S. Riddell, Sylvie Coupaud
Summary: This study aimed to characterize the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. The results showed that the bone responses to SCI are site-specific, with significant changes observed in trabecular and cortical bone at different time points. The findings suggest that the observed bone changes in the rat model are not solely attributed to bone loss, but also to suppressed bone growth and altered spatial distribution of bone. These findings have implications for understanding the bone changes associated with SCI in both adult and pediatric populations.
JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
(2022)