Journal
BIOMATERIALS
Volume 35, Issue 31, Pages 8767-8779Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.06.048
Keywords
Biohybrid-materials; ECM (extracellular matrix); Elastin-like-protein; Genetic engineering; Molecular tecton-libraries; Self-assembly
Funding
- Institute for Macromolecular Chemistry
- Freiburg Institute for Advanced Studies (FRIAS)
- Competence Network of Functional Nanostructures (KFN)
- Baden-Wurttemberg Stiftung
- Ministry of Science, Research and the Arts (MWK) Baden-Wurttemberg
- German Science Foundation (DFG) BIOSS Centre for Biological Signalling Studies [SPP1623, EXC 294]
- Rectorate of the University of Freiburg
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The access to defined protein-based material systems is a major challenge in bionanotechnology and regenerative medicine. Exact control over sequence composition and modification is an important requirement for the intentional design of structure and function. Herein structural- and matrix proteins provide a great potential, but their large repetitive sequences pose a major challenge in their assembly. Here we introduce an integrative one-vector-toolbox-platform (OVTP) approach which is fast, efficient and reliable. The OVTP allows for the assembly, multimerization, intentional arrangement and direct translation of defined molecular DNA-tecton libraries, in combination with the selective functionalization of the yielded protein-tecton libraries. The diversity of the generated tectons ranges from elastine-, resilin, silk- to epitope sequence elements. OVTP comprises the expandability of modular biohybrid-materials via the assembly of defined multi-block domain genes and genetically encoded unnatural amino acids (UAA) for site-selective chemical modification. Thus, allowing for the modular combination of the protein-tecton library components and their functional expansion with chemical libraries via UAA functional groups with bioorthogonal reactivity. OVTP enables access to multitudes of defined protein-based biohybrid-materials for self-assembled superstructures such as nanoreactors and nanobiomaterials, e.g. for approaches in biotechnology and individualized regenerative medicine. (C) 2014 Elsevier Ltd. All rights reserved.
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