Journal
BIOMATERIALS
Volume 31, Issue 24, Pages 6355-6362Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.04.031
Keywords
Isolated hepatocyte; Gene delivery; Intracellular trafficking; Endosomal escape; GALA; MPC polymer
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Special Coordination Funds for Promoting Science and Technology
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We previously reported that modification of GALA peptide on the surface of liposomes enhanced fusion with endosomal membrane, and cytoplasmic release of encapsulated macromolecules. We report herein that an additional coating of GALA-modified liposomes with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer resulted in a two order of magnitude enhancement in the transfection activity of encapsulating plasmid DNA (pDNA). Quantification of the delivered gene copies in whole cells and isolated nuclei revealed that the increase of transfection activity can be attributed to improved efficiencies in cellular uptake and post-nuclear delivery processes. Imaging studies revealed that the intracellular dissociation of pDNA from the lipid envelope is enhanced by GALA modification and further coating with MPC polymer in a stepwise manner. The MPC polymer-coating decreased the zeta-potential of GALA-modified liposomes, suggesting that it assisted in the functional display of negatively charged GALA on the cationic liposomes by providing shielding from mutual electrostatic interactions. Collectively, these data indicate that MPC polymer-coating induced the fusogenic activity of the GALA-modified envelope with endosomes, leading to a more effective cytoplasmic release pDNA. The extensive fusion of the lipid envelope may also reduce electrostatic interactions between mRNA and cationic lipid components, thereby resulting in an enhancement in the translation process. (C) 2010 Elsevier Ltd. All rights reserved.
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