Article
Engineering, Biomedical
Fushi Wang, Xinjie Cai, Ya Shen, Liuyan Meng
Summary: This review discusses the effects of various types of scaffold materials on cell-scaffold interaction in tissue engineering. It elaborates on the influence of the physical, chemical, mechanical, and biological characteristics of scaffold materials on cell-scaffold interaction. The review also examines the role of scaffold components, degradation process, and degraded products in cell-scaffold interactions. Furthermore, it summarizes the latest research progress on cell-scaffold interactions among various engineered tissues or organs in the oral and craniofacial region.
BIOACTIVE MATERIALS
(2023)
Review
Polymer Science
Antony V. V. Samrot, Mahendran Sathiyasree, Sadiq Batcha Abdul Rahim, Robinson Emilin Renitta, Kasirajan Kasipandian, Sivasuriyan Krithika Shree, Deenadhayalan Rajalakshmi, Nagarajan Shobana, Shanmugaboopathi Dhiva, Sasi Abirami, Sridevi Visvanathan, Basanta Kumar Mohanty, Gokul Shankar Sabesan, Suresh V. Chinni
Summary: Biological macromolecules, such as polysaccharides, proteins, and glycoproteins, have been widely used in tissue engineering due to their ability to mimic the extracellular matrix and their biocompatibility. Scaffolds made from these macromolecules have properties like tensile strength, biodegradability, and flexibility. This review discusses the fabrication methods and applications of scaffolds using various biological macromolecules and emphasizes the importance of natural polymers over synthetic ones due to their unique properties.
Review
Biotechnology & Applied Microbiology
Zimu Mao, Baoshi Fan, Xinjie Wang, Ximeng Huang, Jian Guan, Zewen Sun, Bingbing Xu, Meng Yang, Zeyi Chen, Dong Jiang, Jiakuo Yu
Summary: The use of tissue engineering scaffold, especially biomaterials, has shown significant effectiveness in tendon-bone healing and can be enhanced by biological interventions. Current research is mainly focused on animal models, calling for further clinical trials to explore the full potential of these approaches in clinical practice.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Dana Jenkins, Karim Salhadar, Grant Ashby, Anita Mishra, Joy Cheshire, Felipe Beltran, Melissa Grunlan, Sebastien Andrieux, Cosima Stubenrauch, Elizabeth Cosgriff-Hernandez
Summary: The pore size of a scaffold has significant effects on various physical properties in tissue engineering. Manual measurements using ImageJ/FIJI are time-consuming and subjective. In this study, a new MATLAB algorithm called PoreScript was developed and validated using different scaffold fabrication methods. The results demonstrate the accuracy and versatility of PoreScript.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Review
Engineering, Biomedical
Bharti Bisht, Ashley Hope, Anubhab Mukherjee, Manash K. Paul
Summary: The demand for bone grafts is high and complex, with traditional substitutes facing limitations, but the development of 3D printing technology is creating optimism for repairing bone trauma and defects in the future.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Lior Debbi, Barak Zohar, Margarita Shuhmaher, Yulia Shandalov, Idit Goldfracht, Shulamit Levenberg
Summary: By combining living cells, biological hydrogel, and biodegradable synthetic polymer, a multi-scale vascular network within thick, implantable engineered tissues was successfully fabricated in this study. The functionality and patency of the multi-scale vascular network were demonstrated by dextran passage and physiological flow conditions, leading to significant improvements in blood perfusion compared to control micro-scale-vascularized grafts. This innovative approach of designing and fabricating multi-scale vascular architectures within 3D engineered tissues shows promise for both in vitro models and therapeutic translation research.
Article
Biotechnology & Applied Microbiology
Shabnam Anjum, Ting Li, Dilip Kumar Arya, Daoud Ali, Saud Alarifi, Wang Yulin, Zhang Hengtong, P. S. Rajinikanth, Qiang Ao
Summary: Electrospinning was used to fabricate PVP/PVA nanofiber scaffold, and the impact of process parameters and polymer concentration/solvent combinations on the properties of the nanofibers was investigated. The optimized nanofiber formulation showed good performance and excellent biocompatibility, indicating its potential for tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Hui-Yi Hsiao, Gina Alicia Mackert, Yung-Chun Chang, Jia-Wei Liu, Frank Chun-Shin Chang, Jung-Ju Huang
Summary: Current clinical treatments for lymphedema have shown promising results but can cause complications at the donor site. Creating an optimized microenvironment for lymphangiogenesis may be an alternative approach to enhance lymphatic tissue formation. This study investigated the potential of scaffolds containing lymphatic endothelial cells (LECs) in promoting lymphangiogenesis using three in vivo scaffold models exposed to different blood stimuli. The results showed that the arterio-venous (AV) loop model displayed the best lymphangiogenesis compared to the other models. Supplementing LECs is necessary for lymphatic vessel regeneration. The use of an AV loop model as an in vivo scaffold provides an effective approach for engineering lymphatic tissue in the clinical treatment of lymphedema.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Nadiah S. Sulaiman, Andrew R. Bond, Vito D. Bruno, John Joseph, Jason L. Johnson, M-Saadeh Suleiman, Sarah J. George, Raimondo Ascione
Summary: Research has found that decellularizing human saphenous veins (hSV) using 0.01% SDS was effective in producing biocompatible D-hSV grafts. In an animal model, D-hSVs demonstrated excellent mechanical strength and biocompatibility, with no mechanical failure and a 50% patency rate at 4 weeks.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Chemistry, Applied
Tomasz Machalowski, Joanna Idaszek, Adrian Chlanda, Marcin Heljak, Adam Piasecki, Wojciech Swieszkowski, Teofil Jesionowski
Summary: The study developed chitin-based biocomposites through biomimetic ex vivo deposition of calcium carbonate particles in the hemolymph from the cultivated mollusk Cornu aspersum and chitinous matrix from the marine demosponge Aplysina fistularis. The results showed that the biofunctionalized scaffold had about 4 times higher mechanical resistance and exhibited significant topographical changes, indicating its potential for modifying cell delivery systems in future biomedical applications.
CARBOHYDRATE POLYMERS
(2022)
Article
Materials Science, Composites
Zhiyong Ma, Qifan Wang, Wenjia Xie, Wenjie Ye, Linna Zhong, Jile Huge, Ying Wang
Summary: In this study, a PCL/PLGA/HA scaffold with good pore structure was prepared by 3D printing method. The composite scaffold showed good mechanical and cell test performance, indicating potential applications in the field of bone tissue engineering.
POLYMER COMPOSITES
(2021)
Article
Biochemistry & Molecular Biology
Babitha Sumathy, Shiny Velayudhan
Summary: This study introduces a novel method for incorporating hair follicle derived stem cells in micro-pits of a bilayer skin equivalent using microneedles and a gelatin based scaffold. The microneedles are able to penetrate the upper layer of the scaffold and reach the lower layer, mimicking the natural niche of hair follicle stem cells. The bilayer system consists of electrospun layers that represent the epidermis and dermis, resulting in the formation of a complete skin model.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Multidisciplinary
Rui Luo, Fengji Li, Yanan Wang, Huiru Zou, Jianwei Shang, Yaru Fan, Han Liu, Zhaoyuan Xu, Ruixin Li, Hao Liu
Summary: In this study, a biomaterial that combines photothermal therapy and tissue engineering scaffold was developed, which can kill oral squamous cell carcinoma cells and promote the regeneration of mucosal defects. The research showed that the scaffold effectively inhibited cell growth and facilitated the repair of mucosal wounds.
MATERIALS & DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Asefeh Kamrani, Mitra Heydari Nasrabadi, Raheleh Halabian, Masoud Ghorbani
Summary: The fabrication of a 3-layer scaffold using polyurethane and polycaprolactone, along with collagen and zinc ions, shows promising results for dermal regeneration. The composite scaffold containing zinc ions exhibits better wound closure and higher expression of FGF2 and FGFR genes in the healing process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mahmoud Rouabhia, Abdelhabib Semlali
Summary: The use of e-cigarettes increases the growth of S. mutans and the expression of virulent genes. Additionally, it promotes adhesion to, and formation of biofilms on teeth surfaces.
Article
Engineering, Biomedical
Shujun Cui, Mahmoud Rouabhia, Abdelhabib Semlali, Ze Zhang
Summary: Electrical stimulation (ES) has been found effective in promoting wound healing by activating keratinocytes proliferation and secretion of cytokines and growth factors. The results showed that electrically stimulated cells exhibited higher proliferative ability and secretion of various cytokines and growth factors, indicating the potential of ES in benefiting skin wound healing.
BIOMEDICAL MATERIALS
(2021)
Review
Chemistry, Physical
Shiyun Meng, Mahmoud Rouabhia, Ze Zhang
Summary: This work reviews the research on the cellular response to electrical stimulation (ES) and its mechanisms, focusing on potential clinical applications. Different techniques and methodologies have been developed, leading to complications in interpreting and comparing experimental outcomes. The review categorizes these techniques and comments on their strengths and weaknesses. The study finds that ES can enhance cell proliferation, growth, migration, and stem cell differentiation, showing potential for manipulating cellular activities. However, inappropriate parameters or setup can have negative effects, and comparing different parameter sets is challenging. Mechanistic studies are rare and much needed. Despite this, ES combined with advanced materials and nanotechnology is gaining momentum in biomedical research and regenerative medicine.
Article
Microbiology
Neftaha Tazi, Xavier Pigeon, Jerome Mulamba Mbuyi-Boisvert, Simon Giret, Francois Beland, Mahmoud Rouabhia
Summary: The study evaluated the effects of cannabis smoke condensate (CSC) on Candida albicans, a common yeast in the oral cavity. The results suggest that CSC may promote the growth of C. albicans and biofilm formation, while also showing a protective effect against oxidative stress.
Article
Radiology, Nuclear Medicine & Medical Imaging
David Gutierrez, Mahmoud Rouabhia, Javiera Ortiz, Diego Gaviria, Camilo Alfonso, Ana Munoz, Carolina Inostroza
Summary: The study revealed that SCAPs stimulated with low-level laser therapy (LLLT) showed higher proliferation and osteogenic potential, as well as increased expression of VEGF and TGF beta 2 factors. This confirms the promotive effects of LLLT in the preconditioning of SCAPs for regenerative therapies.
JOURNAL OF LASERS IN MEDICAL SCIENCES
(2021)
Article
Cell & Tissue Engineering
Atieh Abedin-Do, Ze Zhang, Yvan Douville, Mireille Methot, Julien Bernatchez, Mahmoud Rouabhia
Summary: The study found that low-intensity electrical stimulation can promote the growth and migration of fibroblasts from diabetic donors and enhance the synthesis of proteolytic enzymes involved in wound healing. This suggests that electrical stimulation has the potential to modulate the wound healing activities of fibroblasts extracted from diabetic skin donors.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Neftaha Tazi, Abdelhabib Semlali, Lionel Loubaki, Abdullah Alamri, Mahmoud Rouabhia
Summary: This study found that cannabis smoke condensate (CSC) has adverse effects on gingival epithelial cells, including affecting cell shape and adhesion, reducing cell viability, and causing apoptosis, autophagy, and oxidative stress. CSC also decreased phosphorylation levels in signaling pathway proteins. These findings suggest that cannabis smoke could impair the immune function of gingival epithelial cells, leading to gingivitis and periodontitis.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Oncology
Lionel Loubaki, Mahmoud Rouabhia, Mohamed Al Zahrani, Abdullah Al Amri, Abdelhabib Semlali
Summary: Cannabinoids, the active components of cannabis, have palliative effects on cancer patients and may also be helpful in treating certain rare forms of cancer. This study investigated the cytotoxic effect of a cannabinoid mixture (CM) on oral cells. The results showed that CM had high cytotoxicity in oral cancer cells at a concentration higher than 0.1 μg/mL but not in normal gingival cells. Low concentrations of CM increased apoptosis, autophagy, antioxidant levels, mitochondrial stress, and DNA damage in oral cells. Furthermore, CM inhibited major signaling pathways involved in cancer progression and cell migration. These findings suggest that cannabinoids could potentially be beneficial in oral cancer therapy.
Article
Oncology
Abdelhabib Semlali, Sofia Papadakos, Camille Contant, Ikram Zouaoui, Mahmoud Rouabhia
Summary: This study evaluated the anti-tumor activity and mechanism of action of rapamycin in oral cancer. The results showed that rapamycin can inhibit cancer cell growth and migration, promote cell apoptosis and autophagy, and inhibit cancer-promoting signaling pathways.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Atieh Abedin-Do, Ze Zhang, Yvan Douville, Mirelle Methot, Julien Bernatchez, Mahmoud Rouabhia
Summary: The prevalence of diabetes is increasing worldwide. This study engineered a diabetic human skin equivalent (DHSE) using fibroblasts and keratinocytes extracted from the skin of diabetic patients and demonstrated that exposure to low-intensity electrical stimulation (ES) can promote diabetic cell activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Miada Abubaker Osman, Nick Virgilio, Mahmoud Rouabhia, Frej Mighri
Summary: In this study, various open-cell composite scaffolds for bone regeneration were developed and characterized. The addition of chitosan (CS) or chitosan-grafted-PLA (CS-g-PLA) increased the flexibility of the PLA matrix and improved compatibility. The modified PLA/CS-g-PLA scaffolds showed high osteoblast cell proliferation, indicating their potential as a solution for bone regeneration.
Article
Polymer Science
Miada Abubaker Osman, Nick Virgilio, Mahmoud Rouabhia, Louis-Etienne Lorenzo, Frej Mighri
Summary: The aim of this study was to develop a solvent-free PLA open-cell porous scaffold for bone tissue engineering using compression molding and a new chemical foaming compound called CFCO. The addition of a CS-g-PLA copolymer during foaming allowed for the copolymer to project towards the surface of the pores and strongly adhere there. At a concentration of 6.90 wt% of CS-g-PLA copolymer, the scaffold showed a significant increase in cell proliferation compared to control samples and scaffolds with CS-g-PLA copolymer dispersed throughout the entire PLA matrix.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Medicinal
Abdelhabib Semlali, Ikram Ajala, Sarra Beji, Mohammed Mousa Al-Zharani, Mahmoud Rouabhia
Summary: This study examines the combined effect of anethole and cisplatin on oral cancer therapy. Results show that anethole enhances cisplatin's inhibitory effect on oral cancer cell proliferation, inhibits cell migration, and induces apoptosis through caspase activation. Additionally, anethole and cisplatin increase the production of reactive oxygen species (ROS) and mitochondrial stress. The combination of anethole and cisplatin also inhibits major cancer signaling pathways, such as MAPKase, beta-catenin, and NF-kappa B, thereby enhancing cisplatin's cancer cell-killing effect and reducing associated side effects.
Article
Biochemistry & Molecular Biology
Abdelhabib Semlali, Sarra Beji, Ikram Ajala, Mohammed Al-Zharani, Mahmoud Rouabhia
Summary: Oral cancer has traditionally been treated with surgery, radiotherapy, chemotherapy, or a combination of these therapies. Recent studies have shown that 3,5-Bis (4-hydroxy-3-methoxybenzylidene)-N-methyl-4-piperidine (PAC), a new curcumin analog, possesses anticancer properties and could be considered a complementary or alternative therapy. The combination of PAC and cisplatin enhances oral cancer cell death by inducing apoptosis, autophagy, and oxidative stress, indicating PAC's potential as a powerful complementary agent to cisplatin in the treatment of gingival squamous cell carcinomas.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Medical Laboratory Technology
Chahrazed Benzaid, Lazhari Tichati, Mahmoud Rouabhia, Sonia Akil Dahdouh
Summary: This study aimed to analyze the different microbial strains present in biological samples collected from patients in the resuscitation unit of Annaba University Hospital Center, and to assess their antimicrobial sensitivity. The results showed that over 59% of the collected samples were microbiologically positive. Candida genus and multi-resistant bacteria to various antibiotics and antifungals were identified as the major causes of nosocomial infections in the medical unit.
ANNALES DE BIOLOGIE CLINIQUE
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
