Article
Chemistry, Inorganic & Nuclear
Hong Wang, Changli Zhang, Zhiyong Jiang, Li Xu, Zhipeng Liu
Summary: In this study, phenothiazine-fused boron complexes were identified as promising scaffolds for ratiometric fluorescent hypochlorite probes. The synthesized complexes demonstrated ultrafast and ratiometric absorption/fluorescence changes for hypochlorite. Additionally, an endoplasmic reticulum-targeting probe was developed and showed excellent real-time imaging ability for both endogenous and exogenous hypochlorite in living cells.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Applied
Weiliang Shen, Guoxing Hu, Hanhan Xu, Wei Sun, Yonghong Hu, Wenge Yang
Summary: Researchers designed a set of ratiometric fluorescent probes to detect SO2 derivatives, with probe NB2 showing a change from red to green fluorescence upon reaction with SO2 and exhibiting excellent optical properties.
Article
Chemistry, Analytical
Ya-Nan Wei, Bo Lin, Yang Shu, Jian-Hua Wang
Summary: A novel fluorescent probe ANET was designed to target mitochondria for detecting Cys, showing advantages of ratiometric fluorescence, high selectivity, and a large Stokes shift. Imaging of Cys was confirmed in HepG2 cells using this probe.
Review
Chemistry, Physical
S. Santhoshkumar, Manivannan Madhu, Wei-Bin Tseng, Wei-Lung Tseng
Summary: Gold nanoclusters (AuNCs) show great potential as ratiometric fluorescent probes due to their adjustable fluorescence wavelengths, biocompatibility, and resistance to photobleaching. By incorporating an additional fluorescence spectral peak, dual-emission AuNC-based probes have been developed to improve signal reproducibility. This review focuses on the preparation methods and applications of ratiometric fluorescent probes derived from AuNCs and other fluorescent nanomaterials or dyes for bioimaging. The challenges and future directions of using AuNCs for ratiometric bioimaging are also discussed.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Spectroscopy
Yamin Yu, Wenlong Sheng, Caiyun Liu, Na Gao, Bin Tian, Hanchuang Zhu, Pan Jia, Zilu Li, Xue Zhang, Kun Wang, Xiwei Li, Baocun Zhu
Summary: A novel ratiometric fluorescent probe CMER was synthesized for efficient detection of mercury ions, showing fast response time and satisfactory bioimaging results.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Xuemei Lin, Wenjing Liu, Shuangyang Xu, Zhanxian Li, Hongyan Zhang, Mingming Yu
Summary: Sulfur dioxide is an environmental pollutant easily absorbed by the human body, and abnormal levels of sulfite and bisulfite have been shown to be related to various diseases. Therefore, developing an effective method to detect bisulfite is necessary. This study designed and synthesized three HSO3- sensitive ratiometric fluorescent probes, which can image HSO3- in living cells, and showed good fluorescence stability and strong anti-interference ability.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Linlin Lv, Weiwei Luo, Quanping Diao
Summary: In this study, a novel phenoxazine-based fluorescent probe BPO-N-3 was developed for detecting H2S with high selectivity and sensitivity. The detection mechanism relies on the ratio of green to red fluorescence signals, and the probe demonstrated successful detection of endogenous and exogenous H2S in living cells. This probe serves as a powerful tool for in-depth exploration of H2S function in various physiological processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Shaohui Han, Hui Zhang, Xiuxiu Yue, Jingpei Wang, Lei Yang, Benhua Wang, Xiangzhi Song
Summary: A ratiometric fluorescent probe CP was developed to selectively and rapidly discriminate between Cys and Hcy by introducing a phenylethynyl group. With distinct ratiometric fluorescence changes induced by the presence of Cys or Hcy, the probe was able to visualize and discriminate between intracellular Cys and Hcy in HeLa cells and zebrafish.
ANALYTICAL CHEMISTRY
(2021)
Article
Spectroscopy
Shuangyu Liu, Xuan Zhao, Qiujuan Ma, Gege Wang, Shuqi Hou, Yijie Ma, Yujie Lian
Summary: A ratiometric fluorescent probe for H2Sn was designed and prepared based on the combination mechanism of intramolecular charge transfer (ICT) and fluorescence resonance energy transfer (FRET). The probe exhibited high selectivity and sensitivity, and was successfully used for imaging in A549 cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Hyerim Kim, Sourav Sarkar, Madhurima Nandy, Kyo Han Ahn
Summary: The rational approach to develop a fluorescent probe for sensing biologically extreme acidity is described. The probe, a push-full type 3-(imidazolyl)benzocoumarin dye, has the lowest pK(a) value among known ratiometric probes. The probe has high quantum yields, chemical stability and aqueous solubility, and it has been successfully used for ratiometric fluorescence imaging of intrabacterial acidity, providing a practical means for studying biological systems under extreme pH conditions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Zhoupeng Zheng, Shumin Feng, Shengyi Gong, Guoqiang Feng
Summary: Gol-CO is an effective fluorescence probe for imaging CO in Golgi apparatus, with high selectivity and sensitivity. It can target Golgi apparatus in living cells and zebrafish, and visualize the level increase of CO under oxidative stress.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Spectroscopy
Wenjun Bai, Yixuan Li, Li Zhao, Ruxin Li, Jiahou Geng, Yang Lu, Yufen Zhao, Jinhui Wang
Summary: In this study, a ratiometric fluorescent probe based on extended 7C-7C conjugation of a triphenylamine unit was designed for the detection of NTR and lysosomes imaging. The probe showed high selectivity, rapid response, and a wide pH range towards NTR. It also exhibited low cytotoxicity in both normal and tumor cells, as well as excellent lysosomes colocalization.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Physical
Xin Guo, Ya-Ru Zhao, Li-Li Zhang, Xiao-Jing Yan, Hai-Bo Liu, Qing-Zhong Li, Cheng-Zhi Xie, Jing-Yuan Xu
Summary: A mGSH-activated fluorescent probe for cancer cells was developed, which showed selective fluorescence quenching and an ON-OFF-ON fluorescence response pattern. The probe was used for selective fluorescence imaging of GSH in zebrafish and mitochondria of living cells.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Physical
Xiaoyuan Wang, Zhiyuan Meng, Shifa Wang, Jiali Kou, Zhonglong Wang, Yiqin Yang
Summary: A novel coumarin-based dual responsive fluorescent probe NNH was synthesized for detection of Cu2+ and H2S. The probe showed significant turn-off fluorescence response to Cu2+ and ratiometric fluorescence response to H2S, with high sensitivity and specificity. It can be used as a sensing tool for Cu2+ and H2S in environmental samples and living organisms.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biochemistry & Molecular Biology
Xiao Sheng, Xinfeng Sun, Yiwen Zhang, Chen Zhang, Shuling Liu, Shouxin Wang
Summary: This study introduces a coumarin-based ratiometric fluorescent probe for the detection of hydrazine (N2H4). The probe shows high selectivity, strong anti-interference capability, low limit of detection, large linear detection range, and wide effective pH interval. It can be used to detect N2H4 in various samples and has low cytotoxicity for monitoring N2H4 in living cells.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)