Article
Pharmacology & Pharmacy
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Patricia Garcia-Garcia, Ricardo Reyes, Maria Isabel Perez-Nunez, Jose A. Riancho, Carmen Evora, Jose Carlos Rodriguez-Rey, Flor M. Perez-Campo
Summary: The study demonstrates a method to enhance the osteogenic potential of MSCs by modulating the expression levels of specific genes. It was found that using Locked Nucleic Acid-Antisense Oligonucleotides and a scaffold releasing low doses of BMP-2 can increase the osteogenic potential of MSCs. By specifically targeting the Wnt pathway modulator Sfrp1, the efficiency of MSC bone regeneration can be improved and osteogenic differentiation in osteoporotic patients' MSCs can be induced.
Article
Pharmacology & Pharmacy
Patricia Garcia-Garcia, Ricardo Reyes, Jose Antonio Rodriguez, Tomas Martin, Carmen Evora, Patricia Diaz-Rodriguez, Araceli Delgado
Summary: The development of scaffolds combining BMP-2 and MMP-10 shows promise for osteoporosis management, with a positive effect on tissue repair for OP mice but a potentially detrimental effect on non-OP animals. Therapeutic molecules can be efficiently encapsulated and controlled released, showing benefits on non-OP cells and similar effects on OP cells.
Review
Endocrinology & Metabolism
Xiaofeng Wang, Fanyuan Yu, Ling Ye
Summary: Recent studies have shown that MSCs play a vital role in bone regeneration, including osteogenic differentiation and providing seed cells. However, factors like drug intake and body senescence can affect MSCs' functions in bone regeneration. Epigenetic regulation has received attention for its heritable nature and involvement in bone metabolism disorders. This review focuses on the effects of epigenetic regulation on osteogenic differentiation, proliferation, and cellular senescence in MSCs and its role in bone regeneration.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Manuel Estevez, Giorgia Montalbano, Alvaro Gallo-Cordova, Jesus G. Ovejero, Isabel Izquierdo-Barba, Blanca Gonzalez, Clarissa Tomasina, Lorenzo Moroni, Maria Vallet-Regi, Chiara Vitale-Brovarone, Sonia Fiorilli
Summary: Nowadays, there is growing interest in developing systems that can guide and influence cell activities for bone regeneration. In this study, researchers combined type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds for bone regeneration. The experimental results showed that the nanostructured scaffolds composed of collagen fibers with embedded SPIONs exhibited enhanced magnetic properties and preserved the viability and adhesion of bone cells.
Article
Materials Science, Multidisciplinary
Xinye Chen, Shuang Wang, Xiaohui Zhang, Yuanman Yu, Jing Wang, Changsheng Liu
Summary: Bone fractures in aging patients often have slow healing rates and high complication rates. Injecting a fibrin gel with sulfated chitosan nanoparticles can improve bone regeneration and reduce inflammation at the fracture site. This gel also regulates macrophage function and effectively treats fractures in postmenopausal mice.
APPLIED MATERIALS TODAY
(2022)
Article
Cell & Tissue Engineering
Tao Hu, Ling Liu, Raymond Wing Moon Lam, Soo Yein Toh, Sunny Akogwu Abbah, Ming Wang, Amit Kumarsing Ramruttun, Kishore Bhakoo, Simon Cool, Jun Li, James Cho-Hong Goh, Hee-Kit Wong
Summary: The study showed that combining BMSCs and low dose BMP-2 delivered by PEC can enhance bone regeneration safety and efficacy, while reducing the effective dose of BMP-2 in a large animal ALIF model. This combination may serve as a bone graft substitute with promising results in fusion outcomes.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Article
Cell Biology
Feng Wang, Wenbo Wang, Lingchi Kong, Li Shi, Mengwei Wang, Yimin Chai, Jia Xu, Qinglin Kang
Summary: This study found that ADM2 accelerates bone regeneration by enhancing the osteogenic differentiation and pro-angiogenic potential of BMSCs, possibly through the NF-κB/β-catenin and AKT/β-catenin pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yumeng Xue, Han-Jun Kim, Junmin Lee, Yaowen Liu, Tyler Hoffman, Yi Chen, Xingwu Zhou, Wujin Sun, Shiming Zhang, Hyun-Jong Cho, JiYong Lee, Heemin Kang, WonHyoung Ryu, Chang-Moon Lee, Samad Ahadian, Mehmet R. Dokmeci, Bo Lei, KangJu Lee, Ali Khademhosseini
Summary: Combining gelatin methacryloyl with silk fibroin can create a mechanically strong and bioactive nanofibrous scaffold for tendon regeneration. The optimized scaffold promotes the growth and tenogenic gene expression of mesenchymal stem cells and enhances tendon tissue regeneration.
Review
Biochemistry & Molecular Biology
Leila Gholami, Vajihe Taghdiri Nooshabadi, Shiva Shahabi, Marzieh Jazayeri, Rana Tarzemany, Zohreh Afsartala, Khatereh Khorsandi
Summary: Oral mesenchymal stem cells (MSCs) and their secretomes play a crucial role in medical tissue engineering and cell free biotherapy due to their accessibility and therapeutic outcomes. Recent studies have focused on the regenerative ability of EVs and CM from MSCs in bone and periodontal defects.
CELL AND BIOSCIENCE
(2021)
Article
Multidisciplinary Sciences
Yuhe Yang, Tianpeng Xu, Ho-Pan Bei, Lei Zhang, Chak-Yin Tang, Ming Zhang, Chenjie Xu, Liming Bian, Kelvin Wai-Kwok Yeung, Jerry Ying Hsi Fuh, Xin Zhao
Summary: This article presents the design and fabrication of high-resolution three-dimensional TPMS scaffolds with biomimicking hyperboloidal topography. These scaffolds show enhanced mechanical strength, support cell attachment and osteogenic differentiation, and promote new bone formation and neovascularization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Khadiga M. Sadek, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Aiah A. El-Rashidy, Christof E. Doerfer, Karim M. Fawzy M. El-Sayed
Summary: Periodontitis is a common chronic inflammatory disease that damages the tissues supporting the teeth. It has three distinct stages: infection, inflammation, and tissue destruction, each requiring specific treatment. Understanding the underlying mechanisms of alveolar bone loss is crucial for effective periodontitis treatment and subsequent reconstruction of the periodontium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Huan Zhou, Lei Zhang, Yue Chen, Chun-Hui Zhu, Fa-Ming Chen, Ang Li
Summary: Bone formation is a complex regeneration process regulated by multiple signaling pathways, with the Hedgehog (Hh) pathway playing a crucial role. Dysregulation of the Hh pathway in adults is associated with bone-related diseases, while during repair processes, it can be reactivated to modulate bone formation. Current therapeutic strategies targeting the Hh cascade for osteogenesis coordination have been reviewed.
CELL PROLIFERATION
(2022)
Article
Materials Science, Biomaterials
Qi Gan, Hao Pan, Wenjing Zhang, Yuan Yuan, Jiangchao Qian, Changsheng Liu
Summary: This study evaluated the osteogenic activity of dual drugs (Dex and BMP-2) loaded into a gelatin scaffold in vivo, and investigated the mechanism of osteogenesis induced by these drugs using C2C12 cells. Results showed that the gelatin scaffold with Dex and BMP-2 significantly accelerated osteogenesis in vivo, and Dex enhanced the osteogenic effect of BMP-2 through activation of the Runx2 pathway.
REGENERATIVE BIOMATERIALS
(2022)
Article
Chemistry, Applied
Ming Jiang, Yuanzhong Pan, Yuanda Liu, Kai Dai, Qinghao Zhang, Jing Wang
Summary: This study utilized sulfated chitosan-based hydrogel scaffolds to promote bone tissue regeneration and vascularization, improving bone repair. In vivo experiments demonstrated the critical role of these scaffolds in angiogenesis and vascularization, as well as their ability to stimulate stem cell proliferation and enhance bone tissue formation. The study also highlighted the angiogenic effects of sulfated chitosan in creating an effective microenvironment for bone repair.
CARBOHYDRATE POLYMERS
(2022)
Article
Materials Science, Biomaterials
M. C. Echave, I Erezuma, N. Golafshan, M. Castilho, F. B. Kadumudi, C. Pimenta-Lopes, F. Ventura, A. Pujol, J. J. Jimenez, J. A. Camara, R. Hernaez-Moya, L. Iturriaga, L. Saenz Del Burgo, I. Iloro, M. Azkargorta, F. Elortza, R. Lakshminarayanan, T. H. Al-Tel, P. Garcia-Garcia, R. Reyes, A. Delgado, C. Evora, J. L. Pedraz, A. Dolatshahi-Pirouz, G. Orive
Summary: In this study, a new osteoconductive scaffold was developed, which reduced the required doses of BMP-2 for bone regeneration. The scaffold showed promising results in promoting cell adhesion, proliferation, and osteogenic differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs). The sustained release of BMP-2 from the scaffold allowed for the treatment of critical-sized osteoporotic bone defects with relatively low growth factor doses.
BIOMATERIALS ADVANCES
(2022)
Article
Biochemical Research Methods
Sandra Pampin-Suarez, Jose Luis Arce-Diego, Olga Tapia, Flor Maria Perez-Campo, Jose Carlos Rodriguez-Rey, Felix Fanjul-Velez
Summary: This study investigated the cytotoxicity of oxazine 4-perchlorate (O4P) on U87 glioma cells at different concentrations and operating room irradiation modes. DNA damage and intracellular localization of O4P were also studied using immunofluorescence and confocal microscopy. The results suggest that concentrations below 100 μM can be considered safe, contributing to the evaluation of O4P as a nerve biomarker.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Medicine, Research & Experimental
Patricia Garcia-Garcia, Ricardo Reyes, Carmen Evora, Araceli Delgado, Jose J. Fernandez, Antonio Hernandez Daranas
Summary: Norzoanthamine (NZ), an alkaloid isolated from marine cnidiaria Zoanthus sp., has shown interesting anti-osteoporotic activity. This in vivo study demonstrates that direct application of NZ into ovariectomized rat bone results in higher bone quality and balanced osteoblast/osteoclast ratio, similar to normal bone. NZ could be a useful adjunct to existing osteoporosis treatments or as a preventive therapy against bone deterioration.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Itsasne Erezuma, Izeia Lukin, Carolina Pimenta-Lopes, Francesc Ventura, Patricia Garcia-Garcia, Ricardo Reyes, M. Rosa Arnau, Araceli Delgado, Nayere Taebnia, Firoz Babu Kadumudi, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Bone tissue engineering has successfully created biologically active hydrogel scaffolds through the combination of different materials, which have shown potential for osteogenesis and angiogenesis in in vitro and in vivo experiments. Despite the need to overcome certain obstacles, these bioengineered structures have demonstrated potential as future bone regeneration treatments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Raquel de la Hoz, Nazely Diban, Maria T. Berciano, Carlos San Emeterio, Ane Urtiaga, Miguel Lafarga, Jose C. Rodriguez-Rey, Olga Tapia
Summary: In this study, we compared the synthesis of polyethyleneimine (PEI) nanoparticles using different methods and found that coaxial electrospraying is a promising technique for the production of therapeutic nanoparticle-based carriers. The nanoparticles synthesized by coaxial electrospraying showed efficient RNA-based drug encapsulation, increased nanoparticle surface availability for functionalization, high transfection efficiency, and hyperactivation of clathrin-independent endocytic pathways as the main intracellular uptake mechanism.
Article
Biochemistry & Molecular Biology
Ana Salas, Silvia Beltran-Flores, Carmen Evora, Ricardo Reyes, Francisco Montes de Oca, Araceli Delgado, Teresa A. Almeida
Summary: This study found that leiomyoma cells can repopulate cell-depleted tissue slices in organ culture, and clusters of CD49b(+) cells were observed in tumor slices. In contrast, CD49b(+) cells were sparsely detected in myometrial slices. Furthermore, leiomyoma cells strongly expressed Ki67 while myometrial cells only had a few stained cells. Based on these findings, it is proposed that activating CD49b(+) stem cells in the myometrium leads to asymmetrical division, giving rise to transit-amplifying KIT+ cells that differentiate to smooth muscle cells. On the other hand, activated leiomyoma CD49b(+) cells symmetrically divide to form clusters of stem cells that divide and differentiate to smooth muscle cells without losing proliferation ability.
Article
Biotechnology & Applied Microbiology
Patricia Garcia-Garcia, Ricardo Reyes, Daniel Garcia-Sanchez, Flor Maria Perez-Campo, Jose Carlos Rodriguez-Rey, Carmen Evora, Patricia Diaz-Rodriguez, Araceli Delgado
Summary: This study successfully enhanced the bone density in osteoporotic mice by using aptamer-functionalized nanoparticles carrying SFRP1 silencing GapmeR, without causing any toxic effects.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Miriam Gonzalez-Gomez, Ricardo Reyes, Ma del Carmen Damas-Hernandez, Xiomara Plasencia-Cruz, Ibrahim Gonzalez-Marrero, Rafael Alonso, Aixa R. Bello
Summary: The study found that prolonged exposure to the environmental estrogenic substance bisphenol-A (BPA) can negatively affect the reproductive axis, resulting in accelerated sexual maturation and a shorter fertile life in offspring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Patricia Diaz-Rodriguez, Patricia Garcia-Garcia, Ricardo Reyes, Carmen Evora, Araceli Delgado
TISSUE ENGINEERING PART A
(2023)
Article
Biochemistry & Molecular Biology
Izeia Lukin, Itsasne Erezuma, Patricia Garcia-Garcia, Ricardo Reyes, Carmen Evora, Firoz Babu Kadumundi, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Bone tissue engineering has addressed the clinical need for bone fractures by using synthetic saponite nanoclays to reinforce gelatin-based hydrogel scaffolds, promoting cell proliferation and differentiation, and serving as a drug delivery system.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
