Journal
BIOMACROMOLECULES
Volume 15, Issue 4, Pages 1204-1215Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm401779r
Keywords
-
Funding
- Development of New Environmental Technology Using Nanotechnology Project of the National Institute of Environmental Science (NIES)
- Ministry of Environment, Japan
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [24760580]
- JSPS A3 Foresight Program
- Kumagai Foundation for Science and Technology
- Grants-in-Aid for Scientific Research [24760580, 25293009] Funding Source: KAKEN
Ask authors/readers for more resources
Thermoresponsive anionic copolymer brushes, poly(N-isopropylacrylamide-co-acrylic acid-co-tert-butylacrylamide) [P(IPAAm-co-AAc-co-tBAAm)], were grafted onto a monolithic silica rod column through surface-initiated atom-transfer radical polymerization (ATRP) to prepare an effective thermoresponsive anionic chromatography matrix. An ATRP initiator was attached to the rod surface. N-Isopropylacrylamide (IPAAm), tert-butyl acrylate (tBA), tert-butylacrylamide (tBAAm), and the ATRP catalyst CuCl/CuCl2/tris[2-(N,N-dimethylamino)ethyl]amine were dissolved in 2-propanol, and the reaction mixture was pumped into the initiator-modified column. After grafting P(IPAAm-co-tBA-co-tBAAm) on the monolithic silica surfaces, deprotection of the tert-butyl group of tBA was performed. Chromatographic analysis showed that the prepared column was able to separate catecholamine derivatives and angiotensin subtypes within a shorter analysis time (5 min) than a silica-bead-packed column modified with the same copolymer brush could. These results indicated that the prepared copolymer-modified monolithic silica rod column may be a promising bioanalytical and bioseparation tool for rapid analysis of basic bioactive compounds and peptides.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available