Article
Chemistry, Multidisciplinary
Francesco Spinozzi, Jean-Pierre Alcaraz, Maria Grazia Ortore, Landry Gayet, Aurel Radulescu, Donald K. Martin, Marco Maccarini
Summary: This study provides a detailed characterization of liposomes containing OprF using small-angle neutron scattering (SANS) and a specific model for data analysis. The quantification of crucial structural information is important for the design and therapeutic applications of liposomal delivery systems.
Article
Biochemistry & Molecular Biology
Bhanu P. Singh, Ryan J. Morris, Tilo Kunath, Cait E. MacPhee, Mathew H. Horrocks
Summary: Many proteins can form diverse polymorphic forms of amyloid and amyloid-like fibers. In this study, the presence of a specific lipid on the neuronal plasma membrane was found to accelerate the aggregation of alpha-synuclein and result in a variety of polymorphic forms. These findings suggest that lipid membranes not only modulate the aggregation kinetics of alpha-synuclein but also play a role in the formation of morphological variants.
Article
Biochemistry & Molecular Biology
Shogo Moriya, Michiko Hanazono, Takeshi Fukuhara, Katsuro Iwase, Nobutaka Hattori, Masaki Takiguchi
Summary: This study investigated the involvement of macrophages/microglia in the formation and spread of alpha-synuclein (αS) fibrils. Transgenic zebrafish expressing αS in macrophages/microglia showed accumulation of αS in neurons. Transcriptome analysis revealed changes in gene expression related to kinases, ubiquitin protein ligases, neuronal activity, and transport. Furthermore, αS fibrils formed from monomers in macrophages and were spread to neurons. The ubiquitin-proteasome system modulated αS fibrils. These findings suggest that macrophages play an essential role in the formation of αS aggregates and the pathogenesis of Parkinson's disease.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Hui Yin, Arielle C. Mensch, Christian A. Lochbaum, Isabel U. Foreman-Ortiz, Emily R. Caudill, Robert J. Hamers, Joel A. Pedersen
Summary: Supported lipid bilayers are important model systems for studying the interactions of proteins, peptides, and nanoparticles with biological membranes. The properties of the solid substrate, such as topography and coating, can affect the formation and properties of supported phospholipid bilayers. The interactions of nanoparticles with supported lipid bilayers depend on the substrate composition.
Article
Biochemistry & Molecular Biology
Yanfei Xie, Jiani Lu, Tiantian Yang, Chao Chen, Yongjie Bao, Luying Jiang, Hua Wei, Xiang Wu, Li Zhao, Shan He, Dongdong Lin, Fufeng Liu, Hao Liu, Xiaojun Yan, Wei Cui
Summary: Phloroglucinol has the ability to degrade pre-formed amyloid aggregates, inhibit seeding during amyloid aggregation, and reduce neurotoxicity, indicating its potential as a novel drug for treating neurodegenerative disorders.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Tianyi Dou, Dmitry Kurouski
Summary: This study utilized nano-infrared imaging technique to investigate the effect of phosphatidylcholine and phosphatidylserine on the structure of alpha-synuclein oligomers. It was found that lipids can alter the secondary structure of the oligomers.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jisoo Lee, Yoon-Jung Kim, La-Mee Choi, Keimin Lee, Hee-Kyung Park, Se-Young Choi
Summary: This study investigated the impact of lipid raft disruption on water secretion in salivary gland cells. The results suggest that lipid rafts play a role in maintaining muscarinic receptor signaling for water secretion but do not directly affect the activation of SOCE or the translocation of AQP5.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Samapan Sikdar, Manidipa Banerjee, Satyavani Vemparala
Summary: This study highlights the crucial role of membrane lipid packing defects in allowing viral peptides to detect, attach, and partition into host cell membranes. The presence of cholesterol significantly alters these defects, affecting the permeability of membranes to viral peptides. The findings provide insights into the general membrane detection mechanism for viroporins.
Article
Biology
Nadia El Mammeri, Olivia Gampp, Pu Duan, Mei Hong
Summary: This study investigates the conformation and assembly of tau aggregates induced by lipid membranes. The results show that tau forms long fibrils on lipid membranes, and the formation of these aggregates requires both membrane curvature and cholesterol.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sevde Puza, Stefanie Caesar, Chetan Poojari, Michael Jung, Ralf Seemann, Jochen S. Hub, Bianca Schrul, Jean-Baptiste Fleury
Summary: Lipid droplets (LDs) are organelles derived from the endoplasmic reticulum (ER) membrane and play a crucial role in lipid storage. The distribution of proteins between phospholipid bilayer and LD monolayer membranes is determined by the collective properties of protein-intrinsic and lipid-mediated features. In this study, researchers produced a freestanding phospholipid bilayer using microfluidics and observed the insertion of micrometer-sized LDs into the bilayer. They found that the LDs presented a lens shape and there was a diffusion barrier between the LD monolayer and the bilayer. This barrier may influence the partitioning of proteins between the ER membrane and LDs in living cells.
Article
Chemistry, Physical
Jean-Baptiste Fleury, Marco Werner, Xavier Le Guevel, Vladimir A. Baulin
Summary: This study investigates how protein corona significantly alters the interaction mode between nanoparticles and lipid bilayers. In the presence of proteins, the length of spikes on the nanoparticle surface exposed to lipid bilayers can be effectively reduced, impacting the dynamics of pore formation. Protein corona is not only crucial for the interaction between NPs and membranes, but may also change their mode of interaction.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Karan Bali, Zeinab Mohamed, Anna Scheeder, Anna-Maria Pappa, Susan Daniel, Clemens F. Kaminski, Roisin M. Owens, Ioanna Mela
Summary: The rise of antibiotic resistance is an increasingly serious global human health issue with significant socioeconomic implications. This study uses outer membrane vesicles to mimic bacterial membranes and explore the composition and properties of complex bilayer systems, providing a high-resolution platform for the development of next-generation antibiotics.
Article
Biophysics
Victoria T. Reichelderfer, Andres F. Chaparro Sosa, Joel L. Kaar, Daniel K. Schwartz
Summary: The interactions between lipid bilayers and insulin can either stabilize or destabilize the protein, preventing or exacerbating the formation of amyloid fibrils.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Nils A. Benning, Jacob Kaestel-Hansen, Fahad Rashid, Sangwoo Park, Raquel Merino Urteaga, Ting-Wei Liao, Jingzhou Hao, James M. Berger, Nikos S. Hatzakis, Taekjip Ha
Summary: Macromolecules can form membrane-less compartments. Nucleosomes and DNA can undergo clustering or condensation to regulate genomic activity. In vitro condensation studies provide information on the physical properties of condensates, but complex kinetic studies of multicomponent condensation require higher resolution methods. In this study, a supported lipid bilayer platform was used with total internal reflection microscopy to observe the movement of DNA and nucleosomes at single-molecule resolution. The results provide insights into the initial condensation events and dissolution of early condensates under physiological conditions.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
Yun-Chiao Yao, Zhongwu Li, Alice J. Gillen, Shari Yosinski, Mark A. Reed, Aleksandr Noy
Summary: Carbon nanotube porins demonstrate excellent biocompatibility and unique water and ion transport properties. Gating transport with external voltage increases control over ion flow and selectivity. The optimal gated CNTP device consists of a semiconducting CNTP inserted into a small membrane patch containing an internally conductive layer.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Chemistry, Physical
Xin Zhou, Dirk Fennema Galparsoro, Anders Ostergaard Madsen, Valeria Vetri, Marco van de Weert, Hanne Morck Nielsen, Vito Fodera
Summary: Amyloid protein aggregates are associated with neurodegenerative diseases and can occur as by-products in protein-based therapeutics. The use of surfactants to stabilize protein formulations can affect protein-protein interactions and alter the properties of the aggregates formed.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Physical
Sara Anselmo, Salvatore Cataldo, Tiziana Avola, Giuseppe Sancataldo, Maria Cristina D'Oca, Tiziana Fiore, Nicola Muratore, Michelangelo Scopelliti, Alberto Pettignano, Valeria Vetri
Summary: This study demonstrates the use of protein particulates as adsorbents for removing Pb2+ ions from aqueous solution. The research reveals the complexity of adsorption mechanisms, influenced by factors such as pH, ionic medium, ionic strength, and temperature.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Camilla Thorlaksen, Heidi S. Schultz, Simon K. Gammelgaard, Wim Jiskoot, Nikos S. Hatzakis, Flemming S. Nielsen, Helene Solberg, Vito Fodera, Christina Bartholdy, Minna Groenning
Summary: This study comprehensively assessed the immunogenicity of human insulin aggregates with varying size, structure, and chemical modifications. The results showed that flexible aggregates with highly altered secondary structure were the most immunogenic in all setups, while compact aggregates with native-like structure were primarily immunogenic in vivo. Additionally, sub-visible aggregates were found to be more immunogenic than sub-micron aggregates, and chemical modifications had no measurable impact on immunogenicity. These findings emphasize the importance of assessing the immunogenicity risk of specific morphological features and provide a reliable workflow for particle analysis in biotherapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Xin Zhou, Anders Wilgaard Sinkjaer, Min Zhang, Henrik Dahl Pinholt, Hanne Morck Nielsen, Nikos S. Hatzakis, Marco van de Weert, Vito Fodera
Summary: Amyloid aggregation is associated with diseases and therapeutic protein formulations. Co-solutes can modulate protein stability and work as inhibitors for drug design. This study uses insulin and polysorbate 80 to analyze the heterogeneous multicomponent system at a single aggregate level and reveals the inhibition mechanism depends on growth pathways. The method is a general tool for physicochemical characterization of self-assembly reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Medicine, Research & Experimental
Ragna G. Diedrichsen, Pernille S. Tuelung, Vito Fodera, Hanne M. Nielsen
Summary: The stereochemistry of carrier peptides affects their interaction with insulin and insulin delivery. The permeation of insulin through the intestinal epithelium is influenced by the degree of insulin-carrier peptide complexation and depends on the stereochemistry of penetramax. The L-form peptides initially decrease epithelial integrity, but this effect is reversible.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Sara Anselmo, Tiziana Avola, Kleopatra Kalouta, Salvatore Cataldo, Giuseppe Sancataldo, Nicola Muratore, Vito Foder, Valeria Vetri, Alberto Pettignano
Summary: Innovative and environmentally friendly protein-based materials were developed for water purification. The adsorption efficiency of these materials for Pb2+ ions can be improved by adjusting the pH of the solution to control the molecular structure and physico-chemical properties. This research provides new knowledge on utilizing plant proteins for the production of new biomaterials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Correction
Chemistry, Physical
Xin Zhou, Dirk Fennema Galparsoro, Anders Ostergaard Madsen, Valeria Vetri, Marco van de Weert, Hanne Morck Nielsen, Vito Fodera
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Vittorio Ferrara, Marco Marchetti, Domenico Alfieri, Lorenzo Targetti, Michelangelo Scopelliti, Bruno Pignataro, Francesco Pavone, Valeria Vetri, Giuseppe Sancataldo
Summary: The use of photocatalysis activated by titanium dioxide nanostructured materials has great potential in various biomedical applications. This study investigated the effects of nitrogen-doped titanium dioxide dispersed in a biocompatible chitosan/PEG hydrogel on protein structures. The results showed the composite material's efficiency in inducing damages on biomolecules under blue light illumination.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Physical
Lasse S. Krog, Jacob J. K. Kirkensgaard, Vito Fodera, Ben J. Boyd, Karlis Berzins
Summary: Low-frequency Raman (LFR) spectroscopy is a viable tool for studying the hydration characteristics of lyotropic liquid crystal systems. The structural changes of monoolein, a model compound, were probed in situ and ex situ, allowing for a comparison between different hydration states. The advantages of LFR spectroscopy were utilized for dynamic hydration analysis, while static measurements showcased the structural sensitivity of LFR spectroscopy. Chemometric analysis distinguished subtle differences between similar self-assembled architectures, correlating directly with small-angle X-ray scattering (SAXS) results, the current gold standard method for determining the structure of such materials.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Sara Anselmo, Giuseppe Sancataldo, Concetta Baiamonte, Giuseppe Pizzolanti, Valeria Vetri
Summary: Continuous progress has been made in the development of new molecules for therapeutic purposes, driven by the need to overcome challenges in molecular stability, biocompatibility, and cellular uptake. Cell-penetrating peptides (CPPs) have emerged as promising tools for drug delivery and antimicrobial/anticancer activity. This study focuses on the analysis of the interactions between the peptide Transportan 10 (TP10) and cancer cell-derived giant plasma membrane vesicles (GPMVs) to understand the molecular mechanisms involved in cellular uptake and cytotoxic effects.
Article
Biochemistry & Molecular Biology
Antoni Kowalski, Cristine Betzer, Sigrid Thirup Larsen, Emil Gregersen, Estella A. Newcombe, Montana Caballero Bermejo, Viktor Wisniewski Bendtsen, Jorin Diemer, Christina Ernstsen, Shweta Jain, Alicia Espina Bou, Annette Eva Langkilde, Lene N. Nejsum, Edda Klipp, Robert Edwards, Birthe B. Kragelund, Poul Henning Jensen, Poul Nissen
Summary: This study shows that monomeric alpha-synuclein can activate plasma membrane Ca2+-ATPase (PMCA) to promote calcium clearance in neurons. The colocalization of alpha-synuclein and PMCA at neuronal synapses suggests a potential physiological function for alpha-synuclein in regulating calcium expulsion.
Article
Chemistry, Physical
Ragna Guldsmed Diedrichsen, Valeria Vetri, Sylvain Prevost, Vito Fodera, Hanne Morck Nielsen
Summary: The cell-penetrating peptide penetratin and its analogues shuffle and penetramax have been investigated for their potential as carrier peptides for oral drug delivery. Through advanced biophysical techniques, it was found that these peptides induced liposome clustering, with distinctively different types of membrane interactions observed. Furthermore, these interactions did not disrupt the liposomes, making them advantageous for drug delivery applications. This detailed insight into peptide-membrane interactions is crucial for understanding the mechanism of peptide-based excipients and the influence of peptide sequence modifications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Medicinal
Jie Zang, Felix Peters, Yves Cambet, Eugenia Cifuentes-Pagano, Munira Mohamed Shishay Hissabu, Christopher M. Dustin, Lars Henrik Svensson, Martin Mariboe Olesen, Mathias Feldt Lomholt Poulsen, Stig Jacobsen, Pernille Sonderby Tuelung, Dilip Narayanan, Annette Eva Langkilde, Michael Gajhede, Patrick J. Pagano, Vincent Jaquet, Frederik Vilhardt, Anders Bach
Summary: In this study, we optimized and explored bivalent small-molecule drugs targeting the NOX2 subunit p47phox and identified compounds with high binding affinities and cellular activities, demonstrating the potential of p47phox as an effective small-molecule target. This has important implications for the research and development of therapeutic drugs for related diseases.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Xuezhi Zhuo, Zeyneb Sener, Aleksei Kabedev, Min Zhao, Anis Arnous, Donglei Leng, Vito Fodera, Korbinian Lobmann
Summary: Protein-based amorphous solid dispersions (ASDs) are a promising approach for enhancing drug solubility. This study investigates the dissolution behavior of different model drugs in protein-based ASDs in various pH media and explores the mechanisms underlying the solubility improvement.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Altair Brito Dos Santos, Siganya Thaneshwaran, Lara Kamal Ali, Cesar Ramon Romero Leguizamon, Yang Wang, Morten Pilgaard Kristensen, Annette E. Langkilde, Kristi A. Kohlmeier
Summary: This study found that a-syn(M) has different effects on female LDT neurons compared to male. In females, a-syn(M) decreases membrane excitability and reduces intracellular calcium, which depends on inhibitory acid transmission and leads to decreased amplitude and frequency of sEPSCs, as well as reduced action potential firing rate. It was also found that a-syn(M) induces higher neurodegeneration in males compared to females through inhibitory amino acid transmission. GABA receptor agonists were associated with reduced cell death in males.
CELL AND BIOSCIENCE
(2023)
