Review
Chemistry, Multidisciplinary
Li He, Mengdi Shang, Zhongkai Chen, Zhaoqi Yang
Summary: Metal-organic frameworks (MOFs) have attracted researchers' attention as distinctive functional materials, constructed by metal ions and organic molecules. In recent years, MOFs have emerged as potential biomedical materials with unique properties. However, their application as nanocarriers for functional nucleic acid delivery in biomedical field has not been summarized. This review introduces the recent developments of MOFs for nucleic acid delivery in various biologically relevant applications, with a focus on cancer therapy, bioimaging, biosensors, and biomolecule separation. The hope is that this review will aid researchers in the biomedical field to develop novel nanocarriers for functional nucleic acid delivery based on the promising material of MOFs.
Article
Pharmacology & Pharmacy
Abhijit Biswas, Maria Maloverjan, Kart Padari, Aare Abroi, Margus Ratsep, Sebastian K. T. S. Warmlander, Juri Jarvet, Astrid Graslund, Vambola Kisand, Runno Lohmus, Margus Pooga
Summary: Cell-penetrating peptides (CPPs) are promising transfection agents for delivering compounds into living cells. The properties of the resulting CPP particles in an aqueous solution depend on the solvent used for dissolving the CPP. Using different methods, such as dynamic light scattering and microscopy, it was found that CPPs dissolved in organic solvents form uniform particles, while those dissolved in water tend to form aggregates and micelles.
Review
Chemistry, Multidisciplinary
Fei Ding, Shuangye Zhang, Qian Chen, Hao Feng, Zhilei Ge, Xiaolei Zuo, Chunhai Fan, Qian Li, Qiang Xia
Summary: Programmable nucleic acid nanotechnology allows the customization of synthetic nucleic acid nanodevices with unprecedented precision, showing potential for precise immunoengineering.
Article
Biochemistry & Molecular Biology
Ryohei Yamaji, Osamu Nakagawa, Yuki Kishimoto, Akane Fujii, Tomoki Matsumura, Taisuke Nakayama, Haruhiko Kamada, Takashi Osawa, Takao Yamaguchi, Satoshi Obika
Summary: This study demonstrates that using oligonucleotides (ONs) containing phenoxazine nucleobases can significantly enhance the therapeutic effect on mouse skeletal muscle diseases, which is of great importance for the treatment of skeletal muscle diseases.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Maxim S. Kupryushkin, Anton Filatov, Nadezhda L. Mironova, Olga A. Patutina, Ivan Chernikov, Elena L. Chernolovskaya, Marina A. Zenkova, Dmitrii Pyshnyi, Dmitry A. Stetsenko, Sidney Altman, Valentin V. Vlassov
Summary: Antisense gapmer oligonucleotides containing phosphoryl guanidine (PG) groups were successfully synthesized and shown to have increased nuclease resistance in serum-containing medium. Replacing two internucleotidic phosphates with PG groups did not decrease cellular uptake, and increasing the number of PG groups affected cellular uptake.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Oncology
Galina Proshkina, Elena Shramova, Anastasiya Ryabova, Liat Katrivas, Clelia Giannini, Daniele Malpicci, Yael Levi-Kalisman, Sergey Deyev, Alexander Kotlyar
Summary: This study demonstrates a methodology to enhance the solubility of peptide nucleic acid (PNA) and delivers it specifically to cancer cells using designed ankyrin repeat protein (DARPin)-functionalized small multilamellar vesicles (SMVs). The positively charged PNA molecules bind electrostatically to the negatively charged phospholipid membranes, encapsulating the PNA in the vesicles. The DARPin-functionalized SMVs can enter HER2-overexpressing cells and cause cell death by interfering with normal cell functioning.
Article
Pharmacology & Pharmacy
Sajid Iqbal, Zhongxi Zhao
Summary: The unique properties of polymers have made significant contributions to drug delivery systems, with block copolymers showing excellent performance in drug targeting. Poly(beta-amino esters) copolymers have demonstrated extraordinary efficiency in designing pre-defined nanostructures and overcoming cellular barriers, with the potential for customized therapeutic functions such as high stability and stimuli-responsive release.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Nanoscience & Nanotechnology
Can Luo, Yuqi Xie, Minze He, Yazhou Li, Yinghao Xia, Lei He, Jili Li, Linlin Wang, Xiaoyan Han, Lili Zhang, Xi Yuan, Zhiqiang Wang, Yanlan Liu, Weihong Tan
Summary: This study introduces a new concept of amphiphilic nucleic acids (ANAs) as a cation-free nucleic acid delivery platform. The ANAs platform demonstrates high siRNA loading capacity, rapid cellular uptake, efficient endosomal escape, and remarkable gene silencing and synergistic inhibitory effects on cancer cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Pharmacology & Pharmacy
Ly Porosk, Ulo Langel
Summary: Cell penetrating peptides (CPPs) are short peptides that can cross cell membranes and facilitate the delivery of drugs and other cargo. Various research tools from chemistry, physics, molecular biology, and engineering have been used to evaluate and characterize CPP-based delivery systems.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Robert Pola, Matej Vicha, Jiri Trousil, Eliska Grosmanova, Michal Pechar, Anna Rumlerova, Martin Studenovsky, Emilie Kucerova, Pavel Ulbrich, Barbora Vokata, Tomas Etrych
Summary: Microbial resistance is a significant issue in modern medicine. Antimicrobial peptides have shown potential as a solution, but their limitations in stability, toxicity, and immunogenic response have hindered their clinical application. In this study, polymer-antibacterial peptide constructs were designed and synthesized. The constructs demonstrated antibacterial activity against both gram-positive and negative bacteria, with the peptide being released from the polymer carrier in response to a pH decrease.
Review
Biotechnology & Applied Microbiology
Zhaoyue Lv, Yi Zhu, Feng Li
Summary: DNA nanomaterials, with unique properties such as biocompatibility and precise control, have shown significant advantages as delivery vectors for nucleic acid-based drugs. This study summarizes the recent progress in the design of DNA-based nanomaterials for cancer therapy, showcasing their applications in controlled nucleic acid delivery.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kerstin Halama, Andreas Schaffer, Bernhard Rieger
Summary: Polyvinylphosphonates are explored for biomedical applications due to their tunable lower critical solution temperature, high biocompatibility, and versatility for post-synthetic modifications. Polymer-analogous transformations were carried out with statistical polyvinylphosphonates containing diethyl vinylphosphonate and diallyl vinylphosphonate, leading to the successful introduction of reactive moieties and subsequent formation of complex polymer conjugates through various reactions. Characterization using NMR, FTIR, UV/Vis spectroscopy, elemental analysis, and gel permeation chromatography confirmed the structural changes and success of the modification approaches.
Review
Chemistry, Physical
Yue Zhang, Yingjie Yu, Yannan Yang, Yue Wang, Chengzhong Yu
Summary: This review discusses the latest developments in designing SiNPs for nucleic acid delivery. The tailored design of SiNPs offers comprehensive functionalities for various nucleic acid applications. Insights for designing innovative delivery nanoplatforms are provided, which have the potential to revolutionize nucleic acid-based therapeutics.
Article
Biochemistry & Molecular Biology
Yasuhito Yokoi, Yugo Kawabuchi, Abdullah Adham Zulmajdi, Reiji Tanaka, Toshiyuki Shibata, Takahiro Muraoka, Tetsushi Mori
Summary: The use of CPP-PNA conjugates helps elucidate the function of unknown proteins in native bacteria, providing a simple and efficient alternative for the lack of screening methods.
Article
Chemistry, Multidisciplinary
Biswa Ranjan Swain, Soumya Ranjan Jena, Swaraj Kumar Beriha, Chandra Sekhara Mahanta, Bibhuti Bhusan Jena, Thumpati Prasanth, Luna Samanta, Rashmirekha Satapathy, Barada P. Dash
Summary: Boron-rich glycodendrimers containing multiple glucose moieties and ortho-carborane clusters were synthesized and found to be completely water soluble due to increased hydrophilicity. In vitro evaluation showed that the glycodendrimer with nine glucose moieties exhibited the highest cytotoxicity against MCF-7 breast cancer cells, with a 600-fold higher potency than cisplatin. The study suggests that these glycodendrimers could serve as tumor-specific anticancer agents.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Polymer Science
D. Das, S. Srinivasan, F. D. Brown, F. Y. Su, A. L. Burrell, J. M. Kollman, A. Postma, D. M. Ratner, P. S. Stayton, A. J. Convertine
Article
Materials Science, Biomaterials
Fang-Yi Su, Jasmin Chen, Hye-Nam Son, Abby M. Kelly, Anthony J. Convertine, T. Eoin West, Shawn J. Skerrett, Daniel M. Ratner, Patrick S. Stayton
BIOMATERIALS SCIENCE
(2018)
Article
Chemistry, Multidisciplinary
Fang-Yi Su, Selvi Srinivasan, Brian Lee, Jasmin Chen, Anthony J. Convertine, Timothy Eoin West, Daniel M. Ratner, Shawn J. Skerrett, Patrick S. Stayton
JOURNAL OF CONTROLLED RELEASE
(2018)
Article
Polymer Science
Hanna Freeman, Selvi Srinivasan, Debobrato Das, Patrick S. Stayton, Anthony J. Convertine
Article
Engineering, Biomedical
Jasmin Chen, Fang-Yi Su, Debobrato Das, Selvi Srinivasan, Hye-Nam Son, Brian Lee, Frank Radella, Dale Whittington, Taylor Monroe-Jones, T. Eoin West, Anthony J. Convertine, Shawn J. Skerrett, Patrick S. Stayton, Daniel M. Ratner
Article
Chemistry, Medicinal
Elaine Limqueco, Daniel Passos Da Silva, Courtney Reichhardt, Fang-Yi Su, Debobrato Das, Jasmin Chen, Selvi Srinivasan, Anthony Convertine, Shawn J. Skerrett, Matthew R. Parsek, Patrick S. Stayton, Daniel M. Ratner
ACS INFECTIOUS DISEASES
(2020)
Article
Chemistry, Multidisciplinary
Duy-Khiet Ho, Clare LeGuyader, Selvi Srinivasan, Debashish Roy, Vladimir Vlaskin, Thomas E. J. Chavas, Ciana L. Lopez, Jessica M. Snyder, Almar Postma, John Chiefari, Patrick S. Stayton
Summary: A new injectable depot platform has been developed with a new mechanism for controlled drug release, aimed at improving patient adherence and releasing tenofovir alafe-namide (TAF) effectively. This platform has the potential for long-acting treatment benefits and sustained drug release profiles over a period of time.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Woojun Kim, Hong Yeol Yoon, Seungho Lim, Patrick S. Stayton, In-San Kim, Kwangmeyung Kim, Ick Chan Kwon
Summary: The non-invasive tracking of T-cells using bioorthogonal click chemistry can predict and induce therapeutic responses in cell therapy.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Thomas E. J. Chavas, Fang-Yi Su, Selvi Srinivasan, Debashish Roy, Brian Lee, Lara Lovelace-Macon, Guilhem F. Rerolle, Elaine Limqueco, Shawn J. Skerrett, Daniel M. Ratner, T. Eoin West, Patrick S. Stayton
Summary: A macrophage-targeted polyciprofloxacin prodrug has been found to be an effective prophylactic measure in highly lethal murine models of aerosolized human pulmonary melioidosis. The prodrug maintained high lung drug levels and targeted an intracellular depot of ciprofloxacin to provide sustained protection over 7 days, despite the pathogen's relative resistance to ciprofloxacin.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Selvi Srinivasan, Debashish Roy, Thomas E. J. Chavas, Vladimir Vlaskin, Duy-Khiet Ho, Ayumi Pottenger, Clare L. M. LeGuyader, Mahdi Maktabi, Pamela Strauch, Conner Jackson, Siobhan M. Flaherty, Hsiuling Lin, Jing Zhang, Brandon Pybus, Qigui Li, Hans E. Huber, Paul A. Burke, David Wesche, Rosemary Rochford, Patrick S. Stayton
Summary: Primaquine and tafenoquine are two 8-aminoquinoline antimalarial drugs approved for malarial radical cure, but they cause hemolytic anemia in G6PD-deficient individuals. By synthesizing these drugs as liver-targeted prodrugs, liver exposure can be maximized, erythrocyte exposure minimized, toxicity reduced, and efficacy improved. Liver-targeted tafenoquine polymers have shown promising results in preclinical studies, with potential for single-dose therapeutic use without the need for G6PD diagnosis.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Engineering, Biomedical
Ciana L. Lopez, Katherine J. Brempelis, James F. Matthaei, Kate S. Montgomery, Selvi Srinivasan, Debashish Roy, Fei Huang, Shannon A. Kreuser, Jennifer L. Gardell, Ian Blumenthal, John Chiefari, Michael C. Jensen, Courtney A. Crane, Patrick S. Stayton
Summary: Engineered immune cells armed with a dual receptor and polymeric prodrug system can efficiently target tumors and their immune-suppressive environments. This approach allows for delivery of drugs to combat tumors and regulation of protein activity in engineered cells, demonstrating potential for antitumor therapy and gene therapy applications.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Shixian Lv, Kefan Song, Albert Yen, David J. Peeler, Dinh Chuong Nguyen, Audrey Olshefsky, Meilyn Sylvestre, Selvi Srinivasan, Patrick S. Stayton, Suzie H. Pun
Summary: The development of a mannosylated polymer for anticancer peptide antigen delivery through self-assembly into micelles has improved the stability and cellular uptake of peptide antigens, resulting in enhanced immune responses against cancer.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Kefan Song, Dinh Chuong Nguyen, Tran Luu, Omeed Yazdani, Debashish Roy, Patrick S. Stayton, Suzie H. Pun
Summary: We introduce Man-VIPER, a self-assembling polymeric peptide delivery platform that targets dendritic cells and enhances antigen cross-presentation. By encapsulating peptide antigens and facilitating endosomal release, Man-VIPER-NR demonstrates superior efficacy in generating antigen-specific cytotoxic T cells.
JOURNAL OF CONTROLLED RELEASE
(2023)
Meeting Abstract
Oncology
Christopher C. DuFort, Ciana L. Lopez, Martin C. Whittle, Vladimir Vlaskin, Aditi Vadodkar, Selvi Srinivasan, Patrick S. Stayton, Sunil R. Hingorani
Article
Pharmacology & Pharmacy
David J. Peeler, Albert Yen, Nicholas Luera, Patrick S. Stayton, Suzie H. Pun
Summary: The study shows that VIPER as a vaccine delivery platform has the potential to enhance vaccine efficacy by disrupting endosomes, but further research is needed to understand how endosomal disruption by VIPER affects vaccination outcomes.
ADVANCED THERAPEUTICS
(2021)
