Article
Materials Science, Multidisciplinary
Niannian Lv, Teng Ma, Huimin Qin, Zhuo-Ran Yang, Yanggui Wu, Danqi Li, Juan Tao, Hao Jiang, Jintao Zhu
Summary: This study presents a designed peptide micelle capable of undergoing in-situ polymerization inside cells, enabling cell labeling and long-term tracking using fluorescence. The polymerization process induces tumor cell apoptosis, increases cell viscosity, and decreases cell motility, potentially inhibiting tumor metastasis.
SCIENCE CHINA-MATERIALS
(2022)
Article
Medicine, Research & Experimental
Qinming Xu, Xing Li, Jingang Yang, Yan Zhang, Xiaocui Deng, Gang Li, Qingmei Yuan
Summary: Four peptide amphiphiles (PA1-4) with different degrees of polymerization were synthesized and characterized. The PAs could self-assemble into spherical micelles in low concentration aqueous solution, adopting random coil conformations. The water solubility of PAs increased with the increase of polymerization degree, and the micelles exhibited controlled drug release effects. The PA2 micelles showed promising potential as drug carriers for cancer therapy.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Arun Dhaka, Ie-Rang Jeon, Olivier Jeannin, Emmanuel Aubert, Enrique Espinosa, Marc Fourmigue
Summary: Successful topochemical polymerization of bis(selenocyanatomethyl)butadyine 1 was achieved through the formation of a co-crystal with 1,2-bis(2-pyridyl)ethylene (2-bpen). The co-crystal underwent polymerization upon heating and the ChB acceptor molecules 2-bpen sublimated during the reaction. The polymer showed enhanced conductivity after iodine doping.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Thomas J. Neal, Nicholas J. W. Penfold, Steven P. Armes
Summary: This study reports a new aqueous polymerization-induced self-assembly (PISA) formulation that utilizes a reverse sequence approach to prepare hydrophobic blocks and produce charge-stabilized latex particles. The latex is then converted into smaller sterically stabilized diblock copolymer nanoparticles, which exhibit thermoresponsive behavior.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Shanbin Qi, Xueting Lu, Wenli Mei, Guanglei Gu, Wen Li, Afang Zhang
Summary: This study investigates the supramolecular assembly of dendronized spiropyrans (DSPs) and the reversible tuning of their amphiphilicity through external stimuli. These dendronized amphiphiles form supramolecular nanospheres in water and exhibit remarkable induced chirality. The assembly can be tuned by photoisomerization and thermal collapse of the dendritic oligoethylene glycols (OEGs).
Article
Chemistry, Multidisciplinary
Manuel G. Ricardo, Peter H. Seeberger
Summary: Biomaterials with improved biological features can be obtained by conjugating glycans to nanostructured peptides. Peptide-glycan chimeras were successfully obtained through the merging of peptide and glycan solid-phase syntheses using a bifunctional monosaccharide. Different chimeras containing various sugars and peptides were prepared and exhibited different self-assembling properties.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Manuel G. Ricardo, Peter H. Seeberger
Summary: Biomaterials with improved biological features can be obtained by conjugating glycans to nanostructured peptides. Creating peptide-glycan chimeras requires superb chemoselectivity. We expedite access to such chimeras by merging peptide and glycan solid-phase syntheses employing a bifunctional monosaccharide.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Yu Chen, Jiaqi Li, Liping Zhao, Huan Chen, Xia Xin, Haihua Wang, Shenshen Li, Wenbing Kang
Summary: Due to the high electronegativity of fluorine atoms, incorporating fluorinated moieties in a polymer can result in unique properties such as low surface energy and self-assembling ability in solution. In this study, a series of amphiphilic copolymers were prepared using three monomers, and their properties were regulated by changing the length of the fluorinated block. One specific copolymer (FAA02) demonstrated low surface tension and self-assembled into pH-sensitive micelles in water.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Florian Mahler, Annette Meister, Carolyn Vargas, Gregory Durand, Sandro Keller
Summary: The use of specific amphiphilic molecules can directly assemble lipids and membrane proteins to form native nanodiscs, rather than mixed micelles. Detailed investigation into the lipid bilayer integrity and membrane protein activity in these nanodiscs reveals gentle effects.
Article
Chemistry, Multidisciplinary
Manuel A. Martinez, Elisa E. Greciano, Jorge Cuellar, Jose M. Valpuesta, Luis Sanchez
Summary: In this study, a highly emissive amphiphilic N-annulated PBI 1 decorated with oligo ethylene glycol (OEG) side chains was synthesized. Compound 1 self-assembles in water to form globular aggregates, demonstrating good solubility and stability in aqueous media. The UV-Vis spectra and solvent denaturation experiments provided insights into the self-assembly process and stability of compound 1 in different solvents.
Review
Polymer Science
Yusuke Sakamoto, Tomoki Nishimura
Summary: The self-assembly of amphiphilic block copolymers is a powerful tool for fabricating nanoparticles with various nanostructures in aqueous solutions. The self-assembly behavior of amphiphilic copolymers is influenced by polymer architecture, and amphiphilic graft copolymers exhibit unique self-assembly behavior, forming nanostructures that cannot be formed by block polymers. However, the self-assembly of amphiphilic graft copolymers is still relatively unexplored.
Article
Polymer Science
Honggui Chen, Xiang Zhang, Ziyu Lin, Rui Zhang, Bingran Yu, Yang Li, Fu-Jian Xu
Summary: This study reports a simple one-step polymerization method for in situ preparation of hydrogels in aqueous solutions, where water-miscible monomers are copolymerized to enhance the mechanical properties of the hydrogels. This strategy provides an effective approach to design mechanically enhanced hydrogels for a wide range of applications.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Article
Polymer Science
Khloe Shuk-Ying Kwan, Ying-Ying Lui, Takashi Kajitani, Franco King-Chi Leung
Summary: This study demonstrates the fabrication of macroscopic functional soft materials through supramolecular assembly without the use of inorganic salts. Anionic and cationic nanotubes of stiff stilbene amphiphiles (SAs) can co-assemble into nanoribbons, which can be further prepared into macroscopic soft materials using inorganic salt solutions or deionized water. These macroscopic soft materials exhibit stability at low concentrations and retain their photoresponsiveness to undergo morphological changes upon photoirradiation.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Jun Zhang, Yining Wang, Junfeng Wang, Youguo Yan, Jiawei Li, Zhen Li
Summary: Vesicle structures assembled from short peptides can be used as carriers for drug delivery. By modifying these peptides with photo-responsive azobenzene moieties, smart vesicles that release cargos under UV or visible light can be created. Molecular simulations have shown that grafting an azo moiety to phenylalanyl-alanine peptides can lead to self-assembling vesicles, and the addition of diphenylalanine improves the efficiency of self-assembly. The formation of vesicles involves nucleation, fusion, and curling, and the isomerization of the azo groups perturbs the packing of the membranes, causing a photo-responsive change in morphology and permeability. These findings have important implications for self-assembly behavior regulation and the design of smart materials.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Guijun Wang, Dan Wang, Anji Chen, Ifeanyi S. Okafor, Lalith Palitha Samankumara
Summary: Glycolipids with diacetylene functional groups are important compounds that can form photopolymerizable gels with stimuli-responsive properties. By introducing diacetylene functional groups at specific positions, a series of N-acetyl-D-glucosamine derivatives were synthesized and found to form self-assembled gels in various solvents, producing red-colored gels. The chemical structures and UV treatment affected the gelation properties of these compounds.
Article
Chemistry, Multidisciplinary
Antoni Llopis-Lorente, Bastiaan C. Buddingh', R. Martinez-Manez, Jan C. M. van Hest, Loai K. E. Abdelmohsen
Summary: This article demonstrates population behavior in microorganisms based on quorum sensing communication. Researchers utilized giant lipid vesicles loaded with sender-receiver machinery (enzymes and responsive biomolecules) to achieve quorum sensing behavior in an artificial cell population. The system allows for the examination of collective output based on cell density, fuel concentration, and proximity, which are important factors controlling natural quorum sensing behavior.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Daan Sondag, Luuk Maartense, Heleen de Jong, Frank F. J. de Kleijne, Kimberly M. Bonger, Dennis W. P. M. Lowik, Thomas J. Boltje, Jan Dommerholt, Paul B. White, Daniel Blanco-Ania, Floris P. J. T. Rutjes
Summary: This study reports a scalable and accessible synthesis method for a new bioorthogonal difunctionalized TCO, demonstrating its potential applications in efficient payload release and cellular toxicity.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Daan Sondag, Stijn Verhoeven, Dennis W. P. M. Lowik, Mark van Geffen, Cornelis van't Veer, Waander L. van Heerde, Thomas J. Boltje, Floris P. J. T. Rutjes
Summary: The blood coagulation cascade is a complex process involving multiple enzymes, cofactors, and substrates, and its dysregulation can lead to bleeding disorders and thrombosis. This review summarizes the key proteases involved in blood clot formation and fibrinolysis, their recognition and hydrolysis of endogenous peptide sequences, and the use of synthetic peptide probes for measuring their activity. The information provided in this review can contribute to the development of novel anticoagulant therapies and specific substrates for point-of-care diagnosis of coagulation pathologies.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Polymer Science
Roy A. J. F. Oerlemans, Jingxin Shao, Sander G. A. M. Huisman, Yudong Li, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Summary: Polymersome nanoreactors as artificial organelles have received significant attention in recent decades, particularly those incorporating biological catalysts. However, there are limited examples of nanoreactors with metal catalysts, and their application in living cells is still restricted. This study presents a biodegradable and biocompatible polymersome nanoreactor platform that can be used as an artificial organelle in living cells. The artificial organelles demonstrated the ability to incorporate tris(triazolylmethyl)amine-Cu(I) complexes in their membrane and effectively catalyze intracellular reactions without compromising cell integrity.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Thijs W. van Veldhuisen, Wiggert J. Altenburg, Madelief A. M. Verwiel, Lenne J. M. Lemmens, Alexander F. Mason, Maarten Merkx, Luc Brunsveld, Jan C. M. van Hest
Summary: Membraneless organelles play a crucial role in spatial organization and regulation of intracellular processes. This study presents a coacervate system that utilizes the 14-3-3 scaffold protein to investigate enzymatically regulated recruitment of 14-3-3-binding proteins. Synthetic coacervates efficiently load 14-3-3 and phosphorylated binding partners, allowing for increased local concentration and controlled release of proteins.
ADVANCED MATERIALS
(2023)
Editorial Material
Biochemical Research Methods
Goncalo J. L. Bernardes, Peng Zou, Zhifei Dai, Erin Lavik, Jan van Hest, Gang Zheng, Nathan Quinn, Christina M. MacLaughlin, Theresa M. Reineke
BIOCONJUGATE CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Daan Sondag, Stijn Verhoeven, Dennis W. P. M. Lowik, Mark van Geffen, Cornelis van't Veer, Waander L. van Heerde, Thomas J. Boltje, Floris P. J. T. Rutjes
Summary: The blood coagulation cascade is a complex physiological process that involves multiple enzymes, cofactors, and substrates, leading to clot formation. Serine proteases play a crucial role, and abnormalities in their activity can result in life-threatening bleeding disorders and thrombosis. This review summarizes the important proteases involved in blood coagulation and fibrinolysis, their recognition and hydrolysis of endogenous peptide sequences, and synthetic peptide probes for measuring their activity. The information in this review can contribute to the development of novel anticoagulant therapies and specific substrates for point-of-care diagnosis of coagulation pathologies.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yvonne H. W. Derks, Melline G. M. Schilham, Mark Rijpkema, Esther M. M. Smeets, Helene I. V. Amatdjais-Groenen, Annemarie Kip, Sanne A. M. van Lith, Jill van de Kamp, J. P. Michiel Sedelaar, Diederik M. Somford, Michiel Simons, Peter Laverman, Martin Gotthardt, Dennis W. P. M. Lowik, Sandra Heskamp, Susanne Lutje
Summary: A new approach called PSMA-tPDT combining fluorescence-guided surgery and tumor-targeted photodynamic therapy (tPDT) has been developed to achieve complete resection of prostate cancer. In vitro and in vivo experiments showed that PSMA-tPDT significantly reduced cell viability, delayed tumor growth, and improved survival rate. Experiments on fresh human PCa tissue also demonstrated the effectiveness of PSMA-tPDT.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Chemistry, Multidisciplinary
Wouter P. van den Akker, Hanglong Wu, Pascal L. W. Welzen, Heiner Friedrich, Loai K. E. A. Abdelmohsen, Rolf A. T. M. van Benthem, Ilja K. Voets, Jan C. M. van Hest
Summary: We constructed pH-responsive bicontinuous nanospheres (BCNs) with nonlinear transient permeability and catalytic activity. These BCNs were made from amphiphilic block copolymers containing pH-responsive groups and loaded with the enzymes urease and horseradish peroxidase (HRP). The transient membrane permeability switch was achieved through the pH-increasing effect of urease, resulting in a dampening behavior of the catalytic output of HRP upon addition of urea.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Alexander B. Cook, Sebastian Novosedlik, Jan C. M. van Hest
Summary: Cells with self-sustaining compartmentalized systems have complex biomolecule and metabolite interactions. Liquid-liquid phase separation plays an important role in achieving biological function. Artificial cell research aims to combine synthetic materials and nonliving macromolecules into ordered assemblies with cell-like functions, with complex coacervates being the best platform for accurately mimicking eukaryotic cytoplasm.
ACCOUNTS OF MATERIALS RESEARCH
(2023)
Article
Biochemical Research Methods
Marleen H. M. E. van Stevendaal, Arjan Hazegh Nikroo, Alexander F. Mason, Jitske Jansen, N. Amy Yewdall, Jan C. M. van Hest
Summary: Oligonucleotide conjugation is a versatile molecular tool for regulating protein activity. This study presents a state-of-the-art labeling strategy by genetically incorporating bioorthogonal groups into proteins to achieve site-specific DNA conjugation. The results demonstrate a new direction for spatiotemporal regulation of chemokine-receptor interactions, which is promising for tissue engineering applications.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Shukun Li, Rui Chang, Luyang Zhao, Ruirui Xing, Jan C. M. van Hest, Xuehai Yan
Summary: This study describes the enhanced TPA fluorescence imaging capacity of self-assembling near-infrared cyanine dye-based nanoprobes based on a photo-oxidation enhanced emission mechanism.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Yigitcan Sumbelli, Alexander F. Mason, Jan C. M. van Hest
Summary: The fast-paced development of regenerative medicine research has led to innovative approaches in tissue engineering applications. Previous studies focused on combining different materials to control cellular behavior and optimizing tissue formation through techniques like 3D bioprinting. However, a higher level of spatiotemporal control on cell-matrix interactions is now recognized as necessary for further advancements in tissue engineering. Integrating artificial cell research with tissue engineering can provide a way to achieve complex tissue development control by equipping artificial cells with mechanisms of cellular functions.
Review
Chemistry, Multidisciplinary
Yanyan Zhu, Shoupeng Cao, Meng Huo, Jan C. M. van Hest, Hailong Che
Summary: Polymersomes are highly stable vesicular nanostructures self-assembled from amphiphilic block copolymers, and have various applications in drug delivery, nanomedicine, biological nanoreactors, and artificial cells. However, polymersomes prepared with high molecular weight components typically exhibit low permeability. This review focuses on the design and strategies for fabricating permeable polymersomes, and highlights their applications and challenges in the field of biomedical utilities.
Article
Chemistry, Multidisciplinary
Antoni Llopis-Lorente, Maaike J. G. Schotman, Heorhii V. Humeniuk, Jan C. M. van Hest, Patricia Y. W. Dankers, Loai K. E. A. Abdelmohsen
Summary: This study explores the relationship between cellular mechanical features and metabolic function in artificial cells and creates an artificial cell platform that displays viscoadaptive behavior through internal supramolecular interactions.