Article
Polymer Science
Gabriela S. Garcia-Briones, Richard Laga, Zulfiya Cernochova, Carely Arjona-Ruiz, Olga Janouskova, Miroslav Slouf, Ognen Pop-Georgievski, Dana Kubies
Summary: Polyelectrolyte nanoparticles (NPs) have been investigated as carriers for protein delivery. The formation of these NPs was studied using a newly synthesized polycationic diblock copolymer and heparin without the use of additives. The encapsulation of heparin-binding proteins achieved high entrapment efficiency and increased protein stability. The released proteins exhibited sustained release and preserved bioactivity. The NPs showed noncytotoxic performance, making them promising candidates for controlled delivery of bioactive proteins in biomedical applications.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Engineering, Biomedical
Yanan Shi, Miaomiao Yin, Yina Song, Tengteng Wang, Shiqi Guo, Xuemei Zhang, Kaoxiang Sun, Youxin Li
Summary: Delivery of peptides or protein drugs via oral route is challenging, but nanoparticles containing chitosan and pHPMA have shown potential for improving the bioavailability of liraglutide. By introducing CSK and HA(2) into the particles, the oral bioavailability of liraglutide can be significantly enhanced, making them a promising option for oral administration of peptides and proteins.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Pharmacology & Pharmacy
Ali Alsuraifi, Essyrose Mathew, Dimitrios A. Lamprou, Anthony Curtis, Clare Hoskins
Summary: This study successfully synthesized amphiphilic APMA monomers and copolymerized them with HPMA to form a thermoresponsive system for encapsulating and releasing model drugs. HPMAco-(APMA-Dansyl 2%) exhibited the highest drug encapsulation ability, while the incorporation of PEG resulted in faster drug release rates.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Polymer Science
Yu-Min Wang, Anna Kalosi, Yuriy Halahovets, Iryna Romanenko, Jiri Slaby, Jiri Homola, Jan Svoboda, Andres de los Santos Pereira, Ognen Pop-Georgievski
Summary: This study compared the anti-fouling properties of poly(HPMA) brushes prepared by chemisorption and surface-initiated polymerization methods, finding that the latter exhibited better anti-fouling performance due to higher grafting density.
Article
Immunology
Michela Manni, Natalia Rodrigues Mantuano, Andreas Zingg, Elisabeth A. Kappos, Anna-Janina Behrens, Jonathan Back, Rainer Follador, Amir Faridmoayer, Heinz Laubli
Summary: This study reveals that non-human Neu5Gc can be incorporated into human skin and has implications for cancer immunotherapy targeting Neu5Gc-containing gangliosides.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Polymer Science
Petr Salek, Daniela Zborilova, Ewa Pavlova, Olga Kockova, Rafal Konefal, Zuzana Moravkova, Olga Janouskova
Summary: In this study, a dispersion polymerization method was developed to prepare cross-linked poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA)-based nanogels. The nanogels were characterized for their size, particle size distribution, morphology, and structure. The biocompatibility of the nanogels was tested using rat mesenchymal stem cells (rMSC) and human foreskin fibroblasts (BJ). The results showed that the nanogels were well-distributed in the cytosol and taken up into lysosomes.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Yamini Bobde, Milan Paul, Tarun Patel, Swati Biswas, Balaram Ghosh
Summary: Delivery of combination chemotherapeutic agents to tumors using nanovesicles shows promise for enhanced tumor suppression and reduced toxicity. These novel micelles exhibit improved anti-tumor efficacy and cytotoxicity, with higher induction of apoptosis and cellular arrest in G2 and S phase compared to traditional drugs.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Applied
Vladimir Sincari, Svetlana Lukas Petrova, Rafal Konefal, Martin Hruby, Eliezer Jager
Summary: The study demonstrated a rapid and eco-friendly RAFT polymerization reaction of the N-(2-hydroxypropyl) methacrylamide (HPMA) monomer under microwave irradiation (MWI). By systematically investigating optimal conditions for the polymerization and utilizing different chain transfer agents, controlled living polymerization was achieved. Additionally, microwave irradiation was shown to facilitate copolymer formation, providing a convenient method for synthesizing PHPMA-based homo- and copolymers.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Yuan Xu, Zhicheng Deng, Yun Chen, Chao Huang, Yong Hu
Summary: A novel mussel-inspired adhesive hydrogel, composed of methacrylated catechol-chitosan (MCCS) and dopamine methacrylate (DMA), was synthesized through photopolymerization. The structure and morphology of the MCCS/DMA adhesive hydrogel were characterized by FTIR, NMR, XRD, TG, and SEM. Rheological and texture properties, swelling and degradation characteristics, as well as the adhesion mechanism of the hydrogels were investigated. The results demonstrated the potential applications of the MCCS/DMA adhesive hydrogel in medical bioadhesives.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Sarita Rani, Umesh Gupta
Summary: The study successfully synthesized three conjugates of HPMA and PCL using a simple and robust synthetic strategy. The results indicate potential applications in the preparation of blends, fibers, etc., and suggest that the explored synthetic scheme could be a suitable approach for the synthesis of HPMA and PCL conjugates in the future.
Article
Chemistry, Physical
Daniel Horak, Karolina Turnovcova, Zdenek Plichta, Dana Marekova, Vladimir Proks, Radek Kaiser, David Netuka, Pavla Jendelova
Summary: In this study, a block copolymer consisting of PHP and PMAH was synthesized and conjugated with Dox and/or RGDS targeting peptide to create nanoparticles that can reduce glioblastoma tumor growth. The nanoparticles were found to effectively decrease tumor size and induce more apoptosis in treated cells compared to untreated cells.
COLLOID AND POLYMER SCIENCE
(2022)
Article
Biophysics
Yamini Bobde, Tarun Patel, Milan Paul, Swati Biswas, Balaram Ghosh
Summary: Polymeric micelles composed of mPEG-b-HPMA copolymer were studied for delivering DOX effectively. The optimized micelle P6-M1 showed small particle size, high drug loading, and promising anticancer effects in breast cancer cell lines. This study demonstrated the potential of mPEG-b-HPMA micelles as a nanomedicine for breast cancer treatment.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Anatomy & Morphology
Medvediev V. Volodymyr, Savosko Serhii, Abdallah M. Ibrahim, Draguntsova G. Natalya, Vaslovych V. Viktoria, Tsymbaliuk Vitaly, Voitenko V. Nana
Summary: This study evaluated the impact of PHPMA-hydrogel on spinal cord regeneration in young rats, showing that the hydrogel helps improve hindlimb locomotor function and promote the growth of nerve fibers.
INTERNATIONAL JOURNAL OF MORPHOLOGY
(2021)
Article
Engineering, Biomedical
Nithusha Kallingal, Rashmi Ramakrishnan, Krishnan Kalliyana
Summary: Three-dimensional bioprinting has emerged as a revolutionary technology for constructing functional tissue equivalents/scaffolds. Bioink design is crucial in this process, and this study reports the formulation and characterization of a bioink system with suitable physicochemical, mechanical, rheological, and biological features. Preliminary results show that the cytocompatible bioink is suitable for 3D bioprinting and biofabrication applications.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2023)
Article
Engineering, Biomedical
Peng Ding, Qianqian Wei, Ning Tian, Xiaoyue Ding, Ling Wang, Bin Wang, Oseweuba Valentine Okoro, Amin Shavandi, Lei Nie
Summary: This study successfully synthesized sialylated chitosan and tyramine-modified gelatin by introducing sialic acid to chitosan and grafting tyramine to gelatin. The interpenetrating polymer network hydrogel was fabricated by blending the two solutions and crosslinking them with horseradish peroxidase. The obtained hydrogel showed excellent cytocompatibility and potential for biomedical applications.
BIOMEDICAL MATERIALS
(2023)
Article
Biochemical Research Methods
Arnaud Masselin, Antoine Petrelli, Maxime Donzel, Sylvie Armand, Sylvain Cottaz, Sebastien Fort
BIOCONJUGATE CHEMISTRY
(2019)
Article
Plant Sciences
Tian Zeng, Luis Rodriguez-Moreno, Artem Mansurkhodzaev, Peng Wang, Willy van den Berg, Virginie Gasciolli, Sylvain Cottaz, Sebastien Fort, Bart P. H. J. Thomma, Jean-Jacques Bono, Ton Bisseling, Erik Limpens
Article
Multidisciplinary Sciences
Feng Feng, Jongho Sun, Guru Radhakrishnan, Tak Lee, Zoltan Bozsoki, Sebastien Fort, Aleksander Gavrin, Kira Gysel, Mikkel B. Thygesen, Kasper Rojkjaer Andersen, Simona Radutoiu, Jens Stougaard, Giles E. D. Oldroyd
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Tomas Allen Rush, Virginie Puech-Pages, Adeline Bascaules, Patricia Jargeat, Fabienne Maillet, Alexandra Haouy, Arthur QuyManh Maes, Cristobal Carrera Carriel, Devanshi Khokhani, Michelle Keller-Pearson, Joanna Tannous, Kevin R. Cope, Kevin Garcia, Junko Maeda, Chad Johnson, Bailey Kleven, Quanita J. Choudhury, Jessy Labbe, Candice Swift, Michelle A. O'Malley, Jin Woo Bok, Sylvain Cottaz, Sebastien Fort, Verena Poinsot, Michael R. Sussman, Corinne Lefort, Jeniel Nett, Nancy P. Keller, Guillaume Becard, Jean-Michel Ane
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Zoltan Bozsoki, Kira Gysel, Simon B. Hansen, Damiano Lironi, Christina Kronauer, Feng Feng, Noor de Jong, Maria Vinther, Manoj Kamble, Mikkel B. Thygesen, Ebbe Engholm, Christian Kofoed, Sebastien Fort, John T. Sullivan, Clive W. Ronson, Knud J. Jensen, Mickael Blaise, Giles Oldroyd, Jens Stougaard, Kasper R. Andersen, Simona Radutoiu
Article
Plant Sciences
Maxime Bonhomme, Sandra Bensmihen, Olivier Andre, Emilie Amblard, Magali Garcia, Fabienne Maillet, Virginie Puech-Pages, Clare Gough, Sebastien Fort, Sylvain Cottaz, Guillaume Becard, Christophe Jacquet
Summary: The study revealed the widespread presence of LCO structures in fungi, raising questions about how legume plants discern different types of LCO signals. Through a genome-wide association study, it was found that while both Nod-LCOs and Fung-LCOs can influence root branching, they operate through distinct molecular mechanisms, with Fung-LCOs showing lower heritability in root response.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Chemistry, Medicinal
Arnaud Masselin, Antoine Rousseau, Stephanie Pradeau, Laure Fort, Rodolphe Gueret, Laurine Buon, Sylvie Armand, Sylvain Cottaz, Luc Choisnard, Sebastien Fort
Summary: Chitin oligosaccharides (COs) have high potential as organic fertilizers in agro-ecological transition, promoting symbiotic relationships between plants and microorganisms for nutrient uptake. Improved access to high-purity COs allows for further exploration of their complex roles in plants.
Article
Chemistry, Multidisciplinary
Antoine Rousseau, Sylvie Armand, Sylvain Cottaz, Sebastien Fort
Summary: Chitin and peptidoglycan fragments are recognized as PAMPs, and long-chain oligosaccharides of beta(1 -> 4)-linked GlcNAc units can activate the innate immune system in plants and mammals. A two-enzyme cascade was reported for synthesizing long-chain GlcNAc oligomers, allowing the milligram-scale synthesis of chitin hexa-, hepta- and octasaccharides with precise size control.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Organic
Antoine Rousseau, Emeline Richard, Isabelle Jeacomine, Sylvie Armand, Sebastien Fort, Sylvain Cottaz
Summary: This study demonstrates a novel selective Zemplen de-O-acetylation of benzyl chitinbioside peracetate for the efficient synthesis of N-acetylmuramyl beta(1 -> 4) N-acetylglucosaminide disaccharide, a key component in the construction of peptidoglycan oligomers. Starting from benzyl chitinbioside, the NAG-NAM disaccharide pentapeptide was successfully prepared in seven steps with an overall yield of 12%.
ASIAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Antoine Rousseau, Julie Michaud, Stephanie Pradeau, Sylvie Armand, Sylvain Cottaz, Emeline Richard, Sebastien Fort
Summary: Soluble fragments of peptidoglycan called muropeptides are released from the cell wall of bacteria, triggering immune responses in mammals and plants. They play a crucial role in inducing antibiotic resistance in bacteria. Efficient production methods for muropeptides are desired to understand their mechanism and develop new antibacterial and immunostimulant agents.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Multidisciplinary Sciences
Sukanya Luang, Xavier Fernandez-Luengo, Alba Nin-Hill, Victor A. Streltsov, Julian G. Schwerdt, Santiago Alonso-Gil, James R. Ketudat Cairns, Stephanie Pradeau, Sebastien Fort, Jean-Didier Marechal, Laura Masgrau, Carme Rovira, Maria Hrmova
Summary: In this study, the researchers investigate the binding and conformational behavior of beta-D-glucosides in the barley beta-D-glucan glucohydrolase. They find that the Trp286/Trp434 clamp plays a crucial role in substrate hydrolysis. Mutations in the clamp affect processive catalysis, and phylogenomic analysis reveals the evolutionary advantage of the tryptophan clamp.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Tomas A. Rush, Joanna Tannous, Matthew J. Lane, Muralikrishnan Gopalakrishnan Meena, Alyssa A. Carrell, Jacob J. Golan, Milton T. Drott, Sylvain Cottaz, Sebastien Fort, Jean-Michel Ane, Nancy P. Keller, Dale A. Pelletier, Daniel A. Jacobson, David Kainer, Paul E. Abraham, Richard J. Giannone, Jesse L. Labbe
Summary: This study demonstrates that lipo-chitooligosaccharides (LCOs) have a significant impact on the fungal metabolic profile of the human pathogen Aspergillus fumigatus, affecting the competitive dynamics between fungi and bacteria. These findings highlight the importance of LCOs in non-plant-symbiotic interactions.
Article
Chemistry, Multidisciplinary
Paul Rivollier, Eric Samain, Sylvie Armand, Isabelle Jeacomine, Emeline Richard, Sebastien Fort
Summary: In this study, efficient gram-scale synthesis of α2,3- and α2,6-sialyllactose oligosaccharides and mimetics was achieved through metabolically engineered bacterial cells grown at high cell density. New Escherichia coli strains were designed to co-express sialic acid synthase and N-acylneuraminate cytidylyltransferase, along with α2,3-sialyltransferase or α2,6-sialyltransferase. The resulting sialylated oligosaccharides showed binding affinity for Sambucus nigra lectin and proved to be stable competitive inhibitors of Vibrio cholerae neuraminidase, indicating their potential for anti-adhesion therapy against influenza viral infections.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Mycology
Manuel I. Villalobos Solis, Nancy L. Engle, Margaret K. Spangler, Sylvain Cottaz, Sebastien Fort, Junko Maeda, Jean-Michel Ane, Timothy J. Tschaplinski, Jesse L. Labbe, Robert L. Hettich, Paul E. Abraham, Tomas A. Rush
Summary: The role of lipo-chitooligosaccharides (LCOs) as signaling molecules in fungal-plant symbiotic relationships is being redefined. New evidence suggests that LCOs are more common in fungi than previously thought and they affect different aspects of fungal growth and development. However, there is still no scientific evidence of the effects of LCOs on Laccaria bicolor.
FRONTIERS IN FUNGAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Akshay Kumar, Hippolyte Durand, Elisa Zeno, Cyril Balsollier, Bastien Watbled, Cecile Sillard, Sebastien Fort, Isabelle Baussanne, Naceur Belgacem, Daniel Lee, Sabine Hediger, Martine Demeunynck, Julien Bras, Gael De Paepe
