The future is cold: cryo-preparation methods for transmission electron microscopy of cells

Spatial organization of the transforming MHC class II compartment

(2010) Hezder E. Nispen Pannerden et al.   BIOLOGY OF THE CELL

CEMOVIS on a pathogen: analysis of Bacillus anthracis spores

(2010) Evelyne Couture-Tosi et al.   BIOLOGY OF THE CELL

Micromachining tools and correlative approaches for cellular cryo-electron tomography

(2010) Alexander Rigort et al.   JOURNAL OF STRUCTURAL BIOLOGY

Alignator: A GPU powered software package for robust fiducial-less alignment of cryo tilt-series

(2010) Daniel Castaño-Díez et al.   JOURNAL OF STRUCTURAL BIOLOGY

The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy

(2010) Michael F. Hayles et al.   JOURNAL OF STRUCTURAL BIOLOGY

Exploring vitreous cryo-section-induced compression at the macromolecular level using electron cryo-tomography; 80S yeast ribosomes appear unaffected

(2010) Jason Pierson et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-electron tomography on vitrified sections: A critical analysis of benefits and limitations for structural cell biology

(2010) Cédric Bouchet-Marquis et al.   MICRON

ISH–IEM: a sensitive method to detect endogenous mRNAs at the ultrastructural level

(2010) Bram Herpers et al.   Nature Protocols

Marker-free image registration of electron tomography tilt-series

(2009) Carlos Sorzano et al.   BMC BIOINFORMATICS

Comparison of different methods for thin section EM analysis of Mycobacterium smegmatis

(2009) C.K.E. BLECK et al.   JOURNAL OF MICROSCOPY

Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: Electrostatic charging for section attachment and implementation of an anti-contamination glove box

(2009) Jason Pierson et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-electron tomography of microtubule–kinesin motor complexes

(2009) Julia Cope et al.   JOURNAL OF STRUCTURAL BIOLOGY

Preservation of high resolution protein structure by cryo-electron microscopy of vitreous sections

(2009) Kasim Sader et al.   ULTRAMICROSCOPY

Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration

(2008) Dagmar Ripper et al.   BIOLOGY OF THE CELL

Direct Visualization of the Outer Membrane of Mycobacteria and Corynebacteria in Their Native State

(2008) B. Zuber et al.   JOURNAL OF BACTERIOLOGY

Compression and crevasses in vitreous sections under different cutting conditions

(2008) H.-M. HAN et al.   JOURNAL OF MICROSCOPY

Comparison of single-particle analysis and electron tomography approaches: an overview

(2008) S. JONIĆ et al.   JOURNAL OF MICROSCOPY

Classification and 3D averaging with missing wedge correction in biological electron tomography☆

(2008) A. Bartesaghi et al.   JOURNAL OF STRUCTURAL BIOLOGY

Tannic acid-mediated osmium impregnation after freeze-substitution: A strategy to enhance membrane contrast for electron tomography

(2008) Nuria Jiménez et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron tomography of early melanosomes: Implications for melanogenesis and the generation of fibrillar amyloid sheets

(2008) I. Hurbain et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of cryo-electron microscopy images does not support the existence of 30-nm chromatin fibers in mitotic chromosomes in situ

(2008) M. Eltsov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Disclosure of the mycobacterial outer membrane: Cryo-electron tomography and vitreous sections reveal the lipid bilayer structure

(2008) C. Hoffmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Electron Cryomicroscopy of E. coli Reveals Filament Bundles Involved in Plasmid DNA Segregation

(2008) J. Salje et al.   SCIENCE

Electron tomography of vitreous sections from cultured mammalian cells

(2007) Manuela Gruska et al.   JOURNAL OF STRUCTURAL BIOLOGY

Markov random field based automatic image alignment for electron tomography

(2007) Fernando Amat et al.   JOURNAL OF STRUCTURAL BIOLOGY