Article
Biotechnology & Applied Microbiology
Jaya Bharati, N. H. Mohan, Satish Kumar, Jayashree Gogoi, Sai Kumar, Bosco Jose, Meeti Punetha, Sanjib Borah, Amit Kumar, Mihir Sarkar
Summary: RNA-Seq was used to identify differentially expressed genes (DEGs) in porcine corpus luteum (CL) during different physiological stages, revealing significant enrichment of gene functions in early, mid, and late stages. Apoptosis was most active in the regressed stage. Pathway analysis indicated lipid metabolism, translation, immune, and endocrine system pathways were associated with CL function. Gene network analysis identified novel hub genes AR, FOS, CDKN1A potentially regulating CL physiology.
Article
Agriculture, Dairy & Animal Science
Cecily Bishop, Vimal Selvaraj, David H. Townson, Joy L. Pate, Milo C. Wiltbank
Summary: The corpus luteum (CL) forms from the cellular remnants of the follicle and produces progesterone required for successful pregnancy. The CL and the embryo have a symbiotic relationship, both relying on each other for survival. The Northeast Multistate Project has contributed to our understanding of the regulation and function of the CL in cattle, providing valuable insights for improving fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Thiago O. Cunha, Leah R. Statz, Rafael R. Domingues, Joao Paulo N. Andrade, Milo C. Wiltbank, Joao Paulo N. Martins
Summary: The objective of this study was to investigate the effects of inducing accessory corpus luteum on follicular and luteal dynamics in lactating Holstein cows. The results showed that inducing accessory corpus luteum not only altered the dynamics of follicles and corpus luteum but also deferred and prolonged the process of luteolysis.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Lihe Liu, Rina Meidan, Francisco Penagaricano, Milo C. Wiltbank
Summary: Research suggests that early subluteolytic PGF pulses impact the luteal transcriptome during natural luteolysis, though not by directly affecting steroidogenesis or inhibiting cellular function. Instead, these early pulses mainly alter pathways related to local steroid and glutathione metabolism.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Ewa Mlyczynska, Marta Kiezun, Patrycja Kurowska, Monika Dawid, Karolina Pich, Natalia Respekta, Mathilde Daudon, Edyta Rytelewska, Kamil Dobrzyn, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska, Joelle Dupont, Agnieszka Rak
Summary: In this review, we summarize the current knowledge about the expression and role of adipokines and neuropeptides in the physiological regulation of corpus luteum function, as well as their potential involvement in pathologies affecting the luteal cells that disrupt the estrous cycle.
Article
Biology
Meeti Punetha, Sai Kumar, Avishek Paul, Bosco Jose, Jaya Bharati, Arvind Sonwane, Jonathan A. Green, Kristin Whitworth, Mihir Sarkar
Summary: This study aimed to better understand the role of EGR1 in transactivation of TGF beta 1 during PGF2 alpha induced luteal regression using buffalo luteal cells. The results showed that EGR1 and TGF beta 1 mRNA expression were significantly upregulated at 12 hours post PGF2 alpha induction, and knocking out EGR1 did not modulate the expression of TGF beta 1. Additionally, the Caspase 3 mRNA expression was significantly increased in EGR1 KO luteal cells treated with PGF2 alpha compared to wild type luteal cells.
BIOLOGICAL RESEARCH
(2021)
Article
Reproductive Biology
Edyta Rytelewska, Marta Kiezun, Ewa Zaobidna, Marlena Gudelska, Katarzyna Kisielewska, Kamil Dobrzyn, Tadeusz Kaminski, Nina Smolinska
Summary: The study found that chemerin stimulates the production of VEGF-A and bFGF in porcine Lc and increases the protein abundance of angiogenesis receptors in these cells. Additionally, chemerin has a modulatory effect on the protein abundance of apoptosis-related factors in porcine Lc, depending on the phase of the cycle.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Sang-Hee Lee, Seunghyung Lee
Summary: The study found that PGF2 alpha affects cell adhesion in bovine luteal theca cells by disrupting cytokeratin, vimentin, and desmoplakin proteins, as well as increasing ROCK protein levels, leading to decreased cell-cell adhesion during corpus luteum regression.
Article
Reproductive Biology
Mariana Diel de Amorim, Sarah A. Bramer, Gayathri D. Rajamanickam, Claudia Klein, Claire Card
Summary: The study investigated the changes in endometrial and luteal gene expression during different reproductive stages and treatments in horses. The results showed that serum LNPEP concentration was higher in pregnant and oxytocin-treated mares compared to diestrous mares. Endometrial PTGES expression was higher in diestrous, pregnant, and oxytocin-treated groups. Carbetocin treatment decreased serum progesterone and LNPEP, increased endometrial PLA2G2C expression, and decreased endometrial PTGES and luteal aromatase. Meclofenamic acid treatment decreased endometrial PLA2G2C, increased endometrial PTGES, and resulted in less OXTR and OXT luteal abundance. The expression of LNPEP is involved in luteal function and pregnancy recognition pathways in horses.
Review
Physiology
Corrine F. Monaco, John S. Davis
Summary: The corpus luteum is a transient endocrine gland in the ovary that produces progesterone for pregnancy. The formation and function of this gland rely on angiogenesis, which establishes the necessary blood flow and microvasculature. During regression, the corpus luteum undergoes structural changes and the blood vessels regress, impacting progesterone production. Factors released during luteolysis play a role in these processes, but the exact mechanisms remain unknown.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Reproductive Biology
Rafael R. Domingues, O. J. Ginther, Victor Gomez-Leon, Thadeu Castro, Milo C. Wiltbank
Summary: This study demonstrates that a single minor PGF treatment can upregulate endogenous PGF synthesis in heifers and mares through distinct regulatory mechanisms, stimulating endogenous PGF synthesis. This results in a transient decrease in P4 in heifers but not in mares, and leads to changes in gene expression in both species.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Granit Thaqi, Bajram Berisha, Michael W. Pfaffl
Summary: The study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, in the corpus luteum (CL) during luteal regression in dairy cows. The results showed significant fluctuations in the expression of adipokines, suggesting their potential roles in regulating the process of luteal regression and subsequent structural changes in the ovary.
Article
Reproductive Biology
Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis
Summary: During the regression of the ovarian corpus luteum, fibroblasts are responsible for rapid matrix remodeling. However, little is known about the role of fibroblasts in the functional or regressing corpus luteum. In this study, it was found that FGF2 activates luteal fibroblasts, leading to collagen production and cell proliferation.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Review
Developmental Biology
Kayhan Yakin, Francesko Hela, Ozgur Oktem
Summary: The corpus luteum is the main source of progesterone, and understanding its signaling pathways is crucial for managing reproductive disorders. This review focuses on the complex mechanisms of progesterone-induced signaling in luteal granulosa cell activity and discusses future research priorities and limitations of the current data.
MOLECULAR HUMAN REPRODUCTION
(2023)