Journal
BIOLOGY OF REPRODUCTION
Volume 89, Issue 4, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.113.109934
Keywords
adrenal medulla; calcium; chloride channel; cyclic adenosine monophosphate (cAMP); ion channels; nitric oxide pathway; oviduct; short-circuit current
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Funding
- CRCG grant from the University of Hong Kong
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The oviduct serves as a site for the fertilization of the ovum and the transport of the conceptus down to the uterus for implantation. In this study, we investigated the presence of adrenomedullin (ADM) and its receptor component proteins in the pig oviduct. The effect of ADM on oviductal secretion, the specific receptor, and the mechanisms involved were also investigated. The presence of ADM and its receptor component proteins in the pig oviduct were confirmed using immunostaining. Short-circuit current (I-sc) technique was employed to study chloride ion secretion in the oviductal epithelium. ADM increased I-sc through cAMP-and calcium-activated chloride channels, and this effect could be inhibited by the CGRP receptor antagonist, hCGRP8-37. In contrast, the nitric oxide synthase inhibitor, L-NG-nitroarginine methyl ester (L-NAME), could not block the effect of ADM on I-sc. In summary, ADM may increase oviductal fluid secretion via chloride secretion independent of the nitric oxide pathway for the transport of sperm and the conceptus.
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