Article
Veterinary Sciences
Maria J. Sanchez-Calabuig, Raul Fernandez-Gonzalez, Meriem Hamdi, Katrien Smits, Angela P. Lopez-Cardona, Consuelo Serres, Beatriz Macias-Garcia, Alfonso Gutierrez-Adan
Summary: The study aimed to analyze the effect of different glucose concentrations on the development and quality of in vitro-produced equine embryos. Results showed that 5 mM glucose during the early culture stages may be preferable to avoid over-activation of embryonic glycolytic pathways. Further studies are needed to determine if this improves embryo developmental competence.
EQUINE VETERINARY JOURNAL
(2021)
Review
Obstetrics & Gynecology
Romualdo Sciorio, Marcos Meseguer
Summary: The main aim of assisted reproductive technology is to achieve a healthy singleton live birth, and the introduction of time-lapse monitoring technology has enabled embryologists to more accurately assess embryo status and implantation potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Cell Biology
Mingwen Wang, Jinfeng Xue, Chanyi Li, Lingbin Qi, Lan Nie, Zhigang Xue
Summary: The optimization of culture conditions is crucial for improving embryo development competence in IVF. Glucose, an important carbon source, has unclear effects on the embryo development microenvironment. LC-MS analysis revealed defined metabolites at different cleavage stages, with glycometabolism playing a critical role. Adding 3 mmol/L glucose significantly improved the development competence and upregulated lipid biosynthesis gene expression at the 2-Cell stage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Chelsea Canon, Anabel Thurman, Albert Li, Carlos Hernandez-Nieto, Joseph A. Lee, Rose Marie Roth, Richard Slifkin, Christine Briton-Jones, Daniel Stein, Alan B. Copperman
Summary: Based on PGT-A testing, we found that 3PN blastocysts that develop to the blastocyst stage and meet the criteria for embryo biopsy have the potential to be euploid. If such blastocysts are selected for transfer, live births can be achieved.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Obstetrics & Gynecology
Xiangfei Wang, Ruolin Mao, Meng Wang, Rui Long, Lei Jin, Lixia Zhu
Summary: This meta-analysis investigates the impact of recryopreservation on embryo viability and the outcomes of in vitro fertilization (IVF). The findings suggest that recryopreservation can result in decreased embryo survival, clinical pregnancy rates, and live birth rates compared to single cryopreservation.
FERTILITY AND STERILITY
(2023)
Article
Endocrinology & Metabolism
Huijun Chen, Jian Li, Sufen Cai, Sha Tang, Suimin Zeng, Chang Chu, Carl-Friedrich Hocher, Benjamin Rosing, Bernhard K. Kramer, Liang Hu, Ge Lin, Fei Gong, Berthold Hocher
Summary: Blastocyst transfer after in vitro fertilization/intracytoplasmic sperm injection increases the risk of developing gestational diabetes mellitus (GDM).
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biology
Minh Tam Le, Thai Thanh Thi Nguyen, Trung Van Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen
Summary: Extending the culture of vitrified-warmed cleavage embryos for blastocyst transfer showed significantly better pregnancy outcomes compared to day 2 embryo transfer using cryopreserved embryos, especially for patients with previous cleavage embryo transfer failures. The development rate of blastocysts in the D2-5 CET group was 52.77%, and the pregnancy outcomes in terms of hCG positivity, clinical pregnancy, and implantation rates were significantly improved compared to the D2 CET group, with no significant differences compared to the D5 CET group.
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Review
Nutrition & Dietetics
Shuang Cai, Shuang Quan, Guangxin Yang, Meixia Chen, Qianhong Ye, Gang Wang, Haitao Yu, Yuming Wang, Shiyan Qiao, Xiangfang Zeng
Summary: Epigenetic modifications play a crucial role in embryonic development, and disruptions caused by nutritional imbalance can impact both embryonic development and adult health. Understanding the regulation of epigenetic modifications in early embryo development is essential for improving reproductive health in mammals through reproductive technologies and nutrition regulation.
ADVANCES IN NUTRITION
(2021)
Review
Obstetrics & Gynecology
Quinton S. Katler, Jennifer F. Kawwass, Bradley S. Hurst, Amy E. Sparks, David H. McCulloh, Ethan Wantman, James P. Toner
Summary: The practice of in vitro fertilization has undergone significant changes over the years, leading to a reduction in the rate of twin pregnancies. This improvement can be attributed to advancements in laboratory techniques, preimplantation genetic testing, and revised guidance on embryo transfer. Single-embryo transfer has become the standard of care, resulting in a dramatic decrease in multifetal pregnancies without compromising treatment effectiveness.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2022)
Review
Cell Biology
Paula R. Chen, Bethany K. Redel, Karl C. Kerns, Lee D. Spate, Randall S. Prather
Summary: The review discusses the application of genetically modified pigs in animal agriculture and human medicine, focusing on the importance of in vitro production and manipulation of embryos. To improve porcine embryo culture systems, consideration of metabolic characteristics, comparisons with other mammalian species, and addressing stressors from environment and parental factors are essential. Progressing stepwise through in vitro oocyte maturation, fertilization, and embryo culture in pigs can help assess current systems and identify areas for improvement.
Article
Obstetrics & Gynecology
Molly M. Quinn, Philip Marsh, Salustiano Ribeiro, Rhodel K. Simbulan, Cristina Hickman, Jorgen Berntsen, Mitchell P. Rosen
Summary: Embryo quality, morphokinetic parameters, and aneuploidy rates were found to be similar in sibling embryos cultured in distinct media systems from the time of gamete isolation.
HUMAN REPRODUCTION
(2022)
Article
Biotechnology & Applied Microbiology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: The study revealed significant differences in protein expression between in vivo and in vitro developed bovine embryos, with embryos developed in vivo internalizing large amounts of oviductin and other proteins early in development, potentially contributing to their higher developmental ability.
Article
Reproductive Biology
Daniel Veraguas-Davila, Maria Francisca Cordero, Soledad Saez, Darling Saez-Ruiz, Alejandro Gonzalez, Fernando Saravia, Fidel Ovidio Castro, Lleretny Rodriguez-Alvarez
Summary: The study evaluated the effects of zona pellucida removal on the in vitro and in vivo development of domestic cat embryos. Results showed that removal of the zona pellucida negatively impacted gene expression and prevented successful implantation of the embryos.
Article
Biochemical Research Methods
Yi Zheng, Yue Shao, Jianping Fu
Summary: This study presents a microfluidic device for modeling human embryo development, derived from human pluripotent stem cells. The resulting structures exhibit molecular and cellular features resembling early post-implantation human embryo development. The microfluidic model is compatible with high-throughput studies and various analytical techniques.
Article
Obstetrics & Gynecology
Kathryn H. Gurner, Jemma Evans, Jennifer C. Hutchison, Alexandra J. Harvey, David K. Gardner
Summary: This study suggests that lactate may play an important role in maternal-fetal dialogue, promoting endometrial receptivity and implantation. Further investigation of the role of lactate in infertile or compromised endometrium could potentially improve pregnancy success.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Genetics & Heredity
Meagan Allen, Lyndon Hale, Daniel Lantsberg, Violet Kieu, John Stevens, Catharyn Stern, David K. Gardner, Yossi Mizrachi
Summary: This study found that post-warming re-expansion and high cell survival rate are associated with higher live birth rate. Embryos with fully expanded blastocysts and high cell survival rate have a higher chance of live birth, but embryos with poor post-warming morphology still have a considerable probability of live birth.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Thi Truong, Alexandra J. Harvey, David K. Gardner
Summary: This study investigated the effect of combined antioxidants on mouse fetal gene expression. The results showed that both in-vitro culture in the presence of 20% oxygen and vitrification of blastocysts significantly perturbed fetal liver and placental gene expression. However, supplementation with A3 reduced the number of differentially expressed genes and altered biological processes, establishing a more in-vivo-like gene expression profile, particularly in the placenta. Additionally, A3 supplementation significantly reduced the expression of genes associated with pre-eclampsia and intrauterine growth restriction.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
David K. Gardner
Summary: By introducing more technologies in the ART laboratory, outcomes such as live birth rate, time to pregnancy, and automation can be improved. New technologies have the potential to assist more patients in becoming parents of healthy children in a shorter period of time.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biochemical Research Methods
Ricardo Correia, Brett Gadsby, Sergiy Korposh, Andrew M. Norris, Barrie r. Hayes-gill, Rishie Sinha, Jonathan G. Hardman, David S. Gardner, Simon Talbot, Daniel Harvey, Julian Mcglashan, Stephen P. Morgan
Summary: This study describes the development of a smart endotracheal tube (ETT) that can measure contact pressure and blood perfusion at the cuff-trachea interface. Testing on a porcine model during mechanical ventilation shows that the device can accurately obtain relevant data, and blood occlusion caused by over-inflation of the cuff can be observed.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Anatomy & Morphology
Jennie N. Jeyapalan, Victoria. James, David S. Gardner, Jennifer H. Lothion-Roy, Nigel P. Mongan, Catrin Sian Rutland
Summary: Research is an essential part of veterinary medicine degrees, and remote learning during the COVID-19 pandemic has affected the performance and experience of students. This study analyzed the outcomes and feedback of students who completed independent research projects, showing that the pandemic cohorts achieved higher grades with increased access to resources while facing challenges in workload management and conceptual exploration.
ANATOMIA HISTOLOGIA EMBRYOLOGIA
(2023)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Dagmar Wilhelm, Alexandra J. Harvey, David K. Gardner
Summary: Exposure to the ketone beta-hydroxybutyrate (beta OHB) impairs preimplantation mouse embryo development, metabolism, epigenetics, and post-transfer viability, with significant effects seen at ketogenic diet (KD)-relevant serum concentrations. These findings suggest a potential detrimental impact on embryo development and viability due to maternal diets that elevate beta OHB levels.
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: Research question: Does the ketone acetoacetate (AcAc) alone, or combined with 13-hydroxybutyrate (13OHB), impact mouse embryo development, metabolism, histone acetylation and viability? The study found that exposure to high levels of ketone acetoacetate and 13-hydroxybutyrate affects metabolism and histone acetylation in embryos, and leads to abnormal fetal development.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
David K. Gardner, Denny Sakkas
Summary: Our ability to maintain viable human embryos in vitro has greatly improved in the past 40 years, leading to higher implantation rates. The future development of embryo culture systems will benefit from new media formulations and more dynamic perfusion-based systems. Furthermore, the integration of artificial intelligence and biomarkers can predict embryo health and viability, improving the effectiveness and cost efficiency of assisted reproductive technology.
FERTILITY AND STERILITY
(2023)
Article
Multidisciplinary Sciences
Hanna J. McLennan, Adam J. Blanch, Samuel J. Wallace, Lesley J. Ritter, Shauna L. Heinrich, David K. Gardner, Kylie R. Dunning, Marty J. Gauvin, Allison K. Love, Jeremy G. Thompson
Summary: This study introduces a microfluidic chip that is designed and completely 3D-printed using two-photon polymerization (2PP). It consists of ten channels and ten retrievable cell sample seats, which can enhance cell-surface interactions at the nanoscale and support cell culture. This technology provides the precision needed for nanoliter fluidic devices constructed from multiple interlocking parts.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Lesia O. O. Kurlak, Paula J. J. Scaife, Louise V. V. Briggs, Fiona Broughton Pipkin, David S. S. Gardner, Hiten D. D. Mistry
Summary: This study aimed to investigate the relationship between the concentrations of trace elements and angiogenic markers in women with pre-eclampsia and their fetuses. The results showed that pregnant women with pre-eclampsia had lower levels of selenium, zinc, and manganese, and higher levels of copper. Additionally, maternal plasma zinc concentration was positively correlated with plasma sFlt-1 concentration, suggesting an imbalance in antioxidant status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: In vitro exposure to ketone bodies fi-hydroxybutyrate (fiOHB) and acetoacetate (AcAc) has a sexually dimorphic impact on gene expression in the fetal liver and placenta of preimplantation mouse embryos.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Jennifer C. Hutchison, Jemma Evans, Tracey A. Edgell, Guiying Nie, David K. Gardner, Lois A. Salamonsen
Summary: The study found that obese infertile women have elevated levels of advanced glycation end-products (AGE) in their uterine environment. The detrimental effects of AGE on endometrial epithelial cells can be reduced with therapeutics and replicated in a more physiologically relevant primary model (organoids).
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)