Article
Cell Biology
Miguel Ricardo Leung, Ravi Teja Ravi, Bart M. Gadella, Tzviya Zeev-Ben-Mordehai
Summary: Mammalian sperm undergo a complex process of capacitation in the female reproductive tract, culminating in acrosomal exocytosis. Cryoelectron tomography reveals the disassembly of paracrystalline structures within the acrosome during this process, supporting a mechanism for gradual dispersal of the acrosomal matrix. The unique membrane fission-fusion pathway in the sperm head maintains cell integrity while transforming the sperm for fertilization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Cameron C. Gardner, Paul F. James
Summary: Na+/H+ exchangers (NHEs) are important regulators of pH in eukaryotic cells and play crucial roles in sperm function and male fertility. Mammalian sperm express multiple NHE isoforms that contribute to various aspects of sperm physiology and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Steven Serafini, Cristian O'Flaherty
Summary: Spermatozoa undergo capacitation, a process involving biochemical and morphological changes to enable fertilization. Reactive oxygen species (ROS) play a dual role in capacitation, regulating cellular processes at physiological levels but causing damage at high concentrations. Understanding redox signaling and phosphorylation events in sperm is crucial for fertility and developing treatments for male infertility.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Biochemistry & Molecular Biology
Haim Breitbart, Elina Grinshtein
Summary: Capacitation is a process in which mammalian sperm undergo a series of biochemical reactions in the female reproductive tract, protecting the sperm from spontaneous acrosome reaction and ensuring successful fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Reproductive Biology
Ryuzo Yanagimachi
Summary: Significant progress has been made in the analysis and manipulation of mammalian gametes and fertilization processes over the past 60 years. However, there are still many intriguing aspects that require further exploration. Mammalian fertilization is a complex process that involves the meeting of eggs and sperm at the right time and position within the female reproductive tract. In this review, the author discusses various aspects of mammalian fertilization, highlights their laboratory's contributions to the field, and addresses remaining enigmas and mysteries.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Ying Yang, Fangyi Chen, Kun Qiao, Hua Zhang, Hui-Yun Chen, Ke-Jian Wang
Summary: The AMPs SCY2 and scyreprocin in mud crab sperm play a dual role in reproductive immunity and sperm acrosome reaction, with SCY2 directly binding to Ca2+ and both AMPs being essential for progesterone-induced acrosome reaction. This study expands the current understanding of AMPs' roles in reproduction by demonstrating their direct involvement in sperm acrosome reaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Andres Aldana, Jorge Carneiro, Gustavo Martinez-Mekler, Alberto Darszon
Summary: The acrosome reaction is a crucial exocytotic process in mammalian fertilization, involving various physiological changes that enable sperm to interact and fuse with eggs. However, questions remain regarding the molecular mechanisms regulating the acrosome reaction and the impact of physiological heterogeneity within sperm populations on the proportion capable of undergoing the reaction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yun Chen, Kai Wang, Shouquan Zhang
Summary: This study used reliable experimental materials and methods to detect the effects of osteopontin (OPN) on boar sperm in vitro. The results showed that OPN significantly improved sperm motility, acrosome reaction ability, and embryo division.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Article
Developmental Biology
Gabriela Carrasquel Martinez, Andres Aldana, Jorge Carneiro, Claudia Lydia Trevino, Alberto Darszon
Summary: Mammalian sperm capacitation, involving biochemical and physiological modifications, is a crucial step for successful fertilization. This study showed that the acrosome pH gradually increases during human sperm capacitation, and the V-ATPase plays a key role in regulating the acrosome acidity. These findings provide important insights into the factors influencing sperm fusion and fertilization.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Fisheries
Bernadett Pataki, Akos Horvath, Gergely Meszaros, Nevena Kitanovic, Andras Acs, Arpad Hegyi, Jozsef Molnar, Balazs Csorbai, Bela Urbanyi
Summary: Absorbance and computer-assisted sperm analysis (CASA) were used to assess the sperm concentration of common carp, and the effect of different concentrations on post-thaw sperm parameters and fertilizing capacity were tested. The results showed that both spectrophotometry and CASA are suitable for assessing common carp sperm concentration, and maximizing sperm concentration before cryopreservation results in higher fertilization.
AQUACULTURE REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Filip Benko, Dana Urminska, Michal Duracka, Eva Tvrda
Summary: This review provides an overview of specific ion channels and transporters located in the mammalian sperm plasma membrane and their functional roles in sperm development and function. It discusses the potential therapeutic targets for infertility management.
Review
Cell Biology
Katherine A. Maniates, Andrew Singson
Summary: Complementary forward and reverse genetic approaches have led to a recent surge in the discovery of fertilization genes in various model systems. The number of genetically validated gamete surface molecules has more than doubled in the past few years, with all sperm fertilization genes encoding transmembrane or secreted molecules. However, the discovery of genes encoding oocyte molecules has lagged behind. This review discusses potential experimental biases and biological reasons that may have hindered the identification of egg fertilization genes, as well as current strategies to overcome these obstacles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Pauline Arbogast, Guillaume Gauchotte, Romane Mougel, Olivier Morel, Ahmed Ziyyat, Mikael Agopiantz
Summary: Neurotensin (NTS) is a peptide that was discovered in 1973 and has been extensively studied in various fields, particularly in oncology. This review focuses on its involvement in reproductive functions, specifically its role in ovulation and sperm function. NTS acts in an autocrine and paracrine manner, influencing the acrosome reaction in sperm and potentially improving the outcomes of in vitro fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Dongxue Ma, Mohamed Ali Marey, Masayuki Shimada, Akio Miyamoto
Summary: TLR2 expressed by both cumulus cells and sperm plays a role in regulating the interaction between sperm and oocytes during fertilization. The activation or inhibition of sperm TLR2 affects the binding and penetration abilities of sperm to the zona pellucida, as well as the acrosome reaction. The TLR2 system also regulates the intracellular Ca2+ level in sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
