Article
Endocrinology & Metabolism
Yuqiong Wang, Sheng Wang, Xilin Qian, Yanrong Kuai, Yang Xu
Summary: A single-center, large-cohort, retrospective study compared embryonic development in group culture systems with or without G4 embryos, revealing that multiple G4 embryos have a negative impact on neighboring embryos, while polypronuclear embryos contribute positively. LC-MS/MS analysis of G1-G4 embryo secretome showed that certain proteins secreted by G4 embryos may harm nearby embryos, emphasizing the importance of limiting the number of G4 embryos in group culture settings.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Veterinary Sciences
Hiroyuki Watanabe, Haruka Ito, Ayumi Shintome, Hiroshi Suzuki
Summary: This study examined the effects of oxygen tension and humidity on early embryonic development in mice. The results showed that lower oxygen tension and non-humidified culture conditions better supported preimplantation development of mouse embryos fertilized in vitro, leading to more synchronized development.
EXPERIMENTAL ANIMALS
(2022)
Review
Agriculture, Dairy & Animal Science
Luke Currin, Hernan Baldassarre, Vilceu Bordignon
Summary: Laparoscopic ovum pick-up (LOPU) coupled with in vitro embryo production (IVEP) in prepubertal cattle and buffalo accelerates genetic gain. This article reviews LOPU-IVEP technology in prepubertal Holstein Cattle and Mediterranean Water Buffalo. The recent expansion of genomic-assisted selection has renewed interest and demand for prepubertal LOPU-IVEP schemes; however, low blastocyst development rates has constrained its widespread implementation.
Article
Reproductive Biology
Ting Yang, Xi Yuan, Qingsong Xue, Le Sun, Tairan Xu, Yuan Chen, Deshun Shi, Xiangping Li
Summary: This study used Smart-seq2 technique to investigate the molecular mechanism of symmetric and asymmetric cleavage in early cleavage embryos. The results showed that asymmetric cleavage is associated with altered gene expression, and genes involved in mitochondrial function and cytoskeletal structure, such as Atg4c, Sesn2, Stk11ip, Slc25a6, Cep19 and Cep55, may be the key candidate genes determining the zygote cleavage pattern.
Review
Agriculture, Dairy & Animal Science
Fumie Magata
Summary: In vitro fertilization (IVF) has been increasingly used in bovine embryo production to select cows with high genetic values. Time-lapse monitoring (TLM) has emerged as a new technique that allows frequent and non-invasive imaging of developing embryos. This review outlines the potential application of TLM technology to improve the fertility of bovine IVF embryos and discusses the association between morphokinetic data obtained using TLM and the implantation potential of embryos.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Tommaso Cavazza, Yuko Takeda, Antonio Z. Politi, Magomet Aushev, Patrick Aldag, Clara Baker, Meenakshi Choudhary, Jonas Bucevicius, Grazvydas Lukinavicius, Kay Elder, Martyn Blayney, Andrea Lucas-Hahn, Heiner Niemann, Mary Herbert, Melina Schuh
Summary: Most human embryos are aneuploid and the origin of aneuploidy during early mitotic divisions remains elusive. Clustering of parental genomes with nucleoli in pronuclei is essential for their reliable unification after fertilization, but failure in clustering often leads to chromosome segregation errors and micronuclei, incompatible with healthy embryo development.
Review
Obstetrics & Gynecology
James M. Kemper, Yanhe Liu, Masoud Afnan, Ben W. J. Mol, Dean E. Morbeck
Summary: There is a lack of evidence on embryos derived from oocytes without two pronuclei (2PN) or 'normal fertilization'. A significant difference exists between the development potential of oocytes with abnormal pronuclei and those with 2PN. Most recent studies focus on outcomes of blastocysts derived from non-2PN oocytes, suggesting that they have lower blastocyst rates and slightly reduced implantation potential compared to 2PN oocytes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Shirly Lahav-Baratz, Idit Blais, Mara Koifman, Martha Dirnfeld, Galia Oron
Summary: The aim of this study was to examine the implantation potential of fragmented embryos that underwent morphokinetic evaluation in a time-lapse incubator. A retrospective study analyzed 4210 Day 5 embryos incubated between 2013 and 2019. Results showed that embryo fragmentation and morphokinetics were independent variables for clinical pregnancy achievements. Fragmented embryos had a potential to implant and should be considered for transfer.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2023)
Article
Veterinary Sciences
Hasan Riaz, Muhammad Omer Farooq, Muhammad Farooq
Summary: The study reported successful production of blastocysts and birth of calves in Sahiwal cows of Pakistani origin using OPU-IVEP technique. The cleavage rate of IVF was 68.6% with a blastocyst development rate of 26.3%.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Pin-Yao Lin, Chia-Yun Lin, Ni-Chin Tsai, Fu-Jen Huang, Hsin-Ju Chiang, Yu-Ju Lin, Yu-Ting Su, Kuo-Chung Lan
Summary: Extending the culture of morphologically poor embryos (MPEs) does not increase the live birth rate in fresh transfer cycles, but it results in significantly higher live birth rates when followed by frozen embryo transfer (FET) in younger females.
BIOMEDICAL JOURNAL
(2022)
Article
Biology
Martin Stimpfel, Nina Jancar, Helena Ban-Frangez, Eda Vrtacnik-Bokal
Summary: The latest reports suggest that it may be better to transfer embryos to the uterus on day five instead of day three of preimplantation development, but this may only be applicable when patients are under 36 years old and there are only one-two embryos obtained in the cycle. A retrospective study was conducted to address this issue, analyzing data from IVF/ICSI cycles where one-two embryos were obtained and comparing the outcomes between day three and day five embryo transfer. The study revealed that the birth rate per embryo transfer was significantly higher in the day five group, particularly in patients under 36 years old.
Article
Biology
Minh Tam Le, Thai Thanh Thi Nguyen, Trung Van Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen
Summary: Extending the culture of vitrified-warmed cleavage embryos for blastocyst transfer showed significantly better pregnancy outcomes compared to day 2 embryo transfer using cryopreserved embryos, especially for patients with previous cleavage embryo transfer failures. The development rate of blastocysts in the D2-5 CET group was 52.77%, and the pregnancy outcomes in terms of hCG positivity, clinical pregnancy, and implantation rates were significantly improved compared to the D2 CET group, with no significant differences compared to the D5 CET group.
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Article
Immunology
Zoltan Bognar, Timea Judith Csabai-Tanics, Eva Gorgey, Eva Miko, Zoltan Horvath-Szalai, Julia Szekeres-Bartho
Summary: The role of vitamin D (VD) in reproduction is still inconclusive. Calcitriol treatment at the time of FSH injection or at day 0.5 of pregnancy did not affect conception and implantation in mice, but had adverse effects on oocyte maturation and embryo development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Obstetrics & Gynecology
Nick Macklon, Olga Delikari, Giuseppina Lamanna, Alison Campbell, Simon Fishel, Zaloa Larreategui Laiseca, Marcos Ferrando Serrano, Charlotte Coat, Peter Svalander
Summary: The study found that the temperature at the loaded catheter tip can rapidly drop to ambient temperature during transit, potentially affecting embryo viability. Therefore, maintaining strict temperature control throughout the transfer procedure is essential to improve clinical outcomes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Agriculture, Dairy & Animal Science
Akira Yajima, Hisashi Owada, Suguru Kobayashi, Natsumi Komatsu, Kazuaki Takehara, Maria Ito, Kazuhide Matsuda, Kan Sato, Hisao Itabashi, Satoshi Sugimura, Shuhei Kanda
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
(2017)
Article
Genetics & Heredity
Shoko Ieda, Tomonori Akai, Yoko Sakaguchi, Sumi Shimamura, Atsushi Sugawara, Masahiro Kaneda, Satoko Matoba, Masanori Kagota, Satoshi Sugimura, Hirotsune Kaijima
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2018)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Tadayuki Yamanouchi, Maria Grazia Palmerini, Yutaka Hashiyada, Kei Imai, Robert B. Gilchrist
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2018)
Article
Multidisciplinary Sciences
Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
SCIENTIFIC REPORTS
(2018)
Article
Agriculture, Dairy & Animal Science
Jun Ito, Kanna Meguro, Kazuki Komatsu, Takuya Ohdaira, Rina Shoji, Takahisa Yamada, Satoshi Sugimura, Yohei Fujishima, Akifumi Nakata, Manabu Fukumoto, Tomisato Miura, Hideaki Yamashiro
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
A. O. Hendawy, M. Shirai, H. Takeya, S. Sugimura, S. Miyanari, S. Taniguchi, K. Sato
JOURNAL OF DAIRY SCIENCE
(2019)
Review
Agriculture, Dairy & Animal Science
Amin Omar Hendawy, Mostafa Sayed Khattab, Satoshi Sugimura, Kan Sato
Article
Reproductive Biology
Ryosuke Suzuki, Mai Okada, Hiroki Nagai, Junichi Kobayashi, Satoshi Sugimura
Summary: This study for the first time visualized pronuclei (PN) in delipidated bovine zygotes using time-lapse cinematography (TLC), showing that abnormalities in PN morphokinetics are associated with subsequent embryonic development. Observations suggest that PN morphokinetic abnormalities are linked to developmental competence, highlighting the potential of non-invasive visible light TLC by delipidation as a powerful tool for studying the relationship between PN morphokinetics and embryonic development.
Article
Biochemistry & Molecular Biology
Hiroki Nagai, Mai Okada, Yoko Nagai, Yoshiyuki Sakuraba, Hiroaki Okae, Ryosuke Suzuki, Satoshi Sugimura
Summary: Partial compaction is a self-correction mechanism observed in both human and bovine embryos to avoid aneuploid mosaicism. Excluded blastomeres in embryos are more likely to be aneuploid, while the remaining morulae show euploidy. Abnormal cleavages, such as reverse and direct cleavages, occur more frequently in the excluded blastomeres, suggesting their potential role in the self-correction mechanism during mammalian preimplantation embryo development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Tatsuma Yao, Akane Ueda, Atchalalt Khurchabilig, Daisuke Mashiko, Mikiko Tokoro, Hiroki Nagai, Tei Sho, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
Summary: In assisted reproductive technology (ART)-derived embryos of non-rodent mammals, chromosome segregation errors during early cleavage can have a significant impact on subsequent embryonic development. This study developed a non-invasive live-cell imaging technique to observe chromosome segregation dynamics in bovine preimplantation embryos. The results showed that abnormal chromosome segregation during early cleavage affected the formation of blastocysts, and embryos with severe chromosome segregation errors had prolonged cell cycle durations. The technique also had the potential to select viable ART-derived embryos for medical and livestock production.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Reproductive Biology
Ryosuke Suzuki, Tatsuma Yao, Mai Okada, Hiroki Nagai, Atchalalt Khurchabilig, Junichi Kobayashi, Kazuo Yamagata, Satoshi Sugimura
Summary: Abnormal fertilization, such as multi-PN and pronuclei migration error, is the primary cause of direct cleavage during the first mitosis. Observing direct cleavage helps exclude embryos with abnormal fertilization and improves the success rate of in vitro embryo transfer.
Article
Biotechnology & Applied Microbiology
Amin Omar Hendawy, Satoshi Sugimura, Kan Sato, Mohamed Mohsen Mansour, Ayman H. Abd El-Aziz, Haney Samir, Md. Aminul Islam, A. B. M. Rubayet Bostami, Ahmed S. Mandour, Ahmed Elfadadny, Rokaia F. Ragab, Hend A. Abdelmageed, Amira Mohammed Ali
Summary: Enzymes excreted by rumen microbiome help convert plant materials into nutrients required for animal growth. Factors such as diet, animal age, and health affect the structure of the rumen microbial community. The use of natural supplements, including selenium, has been explored to enhance rumen microbial nutrient supply. This review focuses on the effects of selenium on specific rumen microorganisms, fermentation, and digestibility. Positive effects on various parameters were observed, with selenium nanoparticles showing higher effectiveness. Further studies comparing synthetic selenium nanoparticles and probiotics enriched with sodium selenite are needed.
FERMENTATION-BASEL
(2022)
Article
Agriculture, Dairy & Animal Science
Kazuki Komatsu, Kohsuke Murata, Tsugumi Iwasaki, Syun Tokita, Shiina Yonekura, Satoshi Sugimura, Yohei Fujishima, Akifumi Nakata, Tomisato Miura, Hideaki Yamashiro
Summary: The study reveals that wild large Japanese field mice show dynamic changes in ovarian morphology and estradiol concentrations during the breeding season, indicating a correlation between seasonal follicle development and estradiol regulation. This species is considered as a potential alternative animal model for investigating seasonal reproductive changes and the impacts of environmental changes.
ANIMAL REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Dulama Richani, Robert B. Gilchrist
ANIMAL REPRODUCTION
(2018)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Tomonori Akai, Kei Imai
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2017)