Article
Multidisciplinary Sciences
Diana Monsivais, Takashi Nagashima, Renata Prunskaite-Hyyrylainen, Kaori Nozawa, Keisuke Shimada, Suni Tang, Clark Hamor, Julio E. Agno, Fengju Chen, Ramya P. Masand, Steven L. Young, Chad J. Creighton, Francesco J. DeMayo, Masahito Ikawa, Se-Jin Lee, Martin M. Matzuk
Summary: The study reveals that bone morphogenetic proteins control endometrial receptivity in mice through the ACVR2A and SMAD1/5 signaling pathway, with ACVR2B being dispensable during embryo implantation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Mira Park, So Hee Park, Hyunsun Park, Hye-Ryun Kim, Hyunjung J. Lim, Haengseok Song
Summary: In this study, it was demonstrated that Adamts-1 is a novel target gene of E-2-ER-MAPK-EGR1, critical for embryo implantation in the mouse uterus during early pregnancy.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Vladyslav Bondarenko, Mikhail Nikolaev, Dimitri Kromm, Roman Belousov, Adrian Wolny, Marloes Blotenburg, Peter Zeller, Saba Rezakhani, Johannes Hugger, Virginie Uhlmann, Lars Hufnagel, Anna Kreshuk, Jan Ellenberg, Alexander van Oudenaarden, Anna Erzberger, Matthias P. Lutolf, Takashi Hiiragi
Summary: Using engineering methods, we recreated the implantation process of mouse embryos outside the uterus and revealed the crucial role of physical interactions between the embryo and uterus in embryo morphogenesis. The study found that adhesion between the trophoblast and the uterine matrix is necessary for the transition of the blastocyst to the egg cylinder.
Article
Cell Biology
Yi Yang, Qiu-Yang Zhu, Ji-Long Liu
Summary: This study utilized single-cell RNA sequencing to investigate uterine receptivity in mice, revealing 19 distinct cell clusters and identifying global gene expression changes associated with uterine receptivity in each cell type. The data provide a valuable resource for understanding the molecular mechanisms underlying uterine receptivity.
CELL PROLIFERATION
(2021)
Article
Developmental Biology
Ran Li, Xiao-Tong Song, Si-Wei Guo, Na Zhao, Mei He, Cheng-Qiang He, Nai-Zheng Ding
Summary: YY1 and RTCB may play a role in mouse uterus decidualization and embryo implantation during early pregnancy.
Article
Biochemistry & Molecular Biology
Rui Cao, Zhen-Shan Yang, Sui-Li Hu, Shi-Jin Liang, Shu-Miao Zhang, Song-Qi Zhu, Lin Lu, Cheng-Hong Long, Si-Tong Yao, Yong-Jiang Ma, Xiao-Huan Liang
Summary: This study focused on the molecular mechanism of actin assembly regulation in uterine smooth muscle during early pregnancy. The results showed that Tagln plays a critical role in F-actin polymerization, and decreases in actin assembly are crucial for optimal embryo implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jihyun Lee, Haeun Park, Sohyeon Moon, Jeong-Tae Do, Kwonho Hong, Youngsok Choi
Summary: CD73 is an enzyme that converts AMP into adenosine and is expressed on the surface of various cells, regulating immunity and inflammation. Research shows that the expression of CD73 in the uterus is dynamically regulated by estrogen and progesterone, with a synergistic effect of the two hormones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Rong Li, Xiaoqiu Wang, Zhenyao Huang, Jayani Balaji, Tae Hoon Kim, Tianyuan Wang, Lecong Zhou, Ashley Deleon, Molly E. Cook, Margeaux W. Marbrey, San-Pin Wu, Jae Wook Jeong, Ripla Arora, Francesco J. DeMayo
Summary: The loss of uterine epithelial progesterone receptor (PGR) is crucial for embryo implantation in both humans and mice. This study reveals that the two major isoforms PGRA and PGRB disrupt embryo implantation through FOXO1 pathways in uterine epithelium, and both regulate the LIF-SGK1-FOXO1 signaling pathway.
Article
Developmental Biology
Jennifer Schaefer, Angelos G. Vilos, George A. Vilos, Moshmi Bhattacharya, Andy Babwah
Summary: The study investigated the role of the uterine kisspeptin receptor (KISSIR) in the molecular regulation of implantation in a mouse model, revealing its importance in embryo implantation and healthy pregnancy.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact through Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of-function CTCF mutation in endometrial tumors indicates its importance in uterine homeostasis. Our study reveals that uterine Ctcf plays a key role in progesterone-dependent expression of uterine genes underlying optimal post-pubertal uterine development.
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact via Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of- function CTCF mutation indicates its importance in uterine homeostasis. Deleting Ctcf gene in female reproductive tissues of mice resulted in reduced uterine glands and hypoplastic uteri with decreased endometrial stroma. Ctcf deletion also altered the expression of progesterone-responsive genes, including Ihh, Fst, and Errfi1. Our findings highlight the key role of uterine Ctcf in progesterone-dependent expression and optimal post-pubertal uterine development.
Article
Developmental Biology
Hannah Lufkin, Diana Flores, Zachary Raider, Manoj Madhavan, Madeline Dawson, Anna Coronel, Dhruv Sharma, Ripla Arora
Summary: The movement of pre-implantation embryos is vital for successful pregnancy, but the influence of ovarian hormones on embryo movement is not fully understood. In this study, two mouse models were used to investigate the effects of altered hormonal environment on embryo location. It was found that both progesterone (P4) and estrogen (E2) can modulate embryo movement, and understanding the natural hormonal adaptation in diapause could provide insights into implantation success in hormonally altered conditions. This knowledge has potential applications in improving pregnancy survival and implantation success in clinical settings.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Orthopedics
Yulei Liu, Xiang-Hua Deng, Xueying Zhang, Ting Cong, Daoyun Chen, Arielle Jordan Hall, Liang Ying, Scott A. Rodeo
Summary: The Indian hedgehog signaling pathway plays an important role in the development and healing process of supraspinatus tendon tendinopathy in a mouse shoulder impingement model. With the relief of impingement, tendon pathology gradually improves, while the signaling pathway and its downstream mediator GLI1 continue to increase.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Review
Obstetrics & Gynecology
Jianghong Cheng, Zizhuo Sha, Junyang Li, Bixuan Li, Xianyang Luo, Zhiming Zhang, Yi Zhou, Shuai Chen, Yang Wang
Summary: Embryo implantation and decidualization are vital for successful pregnancy, and defects in these processes can lead to adverse pregnancy outcomes. Around 75% of failed pregnancies are caused by implantation failure or defects. This article reviews recent advances in understanding the molecular mechanisms of estrogen and progesterone regulation in establishing uterine receptivity, blastocyst implantation, and decidualization, with the aim of developing strategies to prevent or treat implantation defects and improve pregnancy rates.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lin Lu, Yingni Chen, Zhenshan Yang, Shijin Liang, Songqi Zhu, Xiaohuan Liang
Summary: During decidualization in rodents, uterine stromal cells undergo reprogramming to differentiate into different cell types, forming primary and secondary decidual zones. Secondary decidual zones are accompanied by angiogenesis. Estrogen, both ovarian and locally synthesized, is essential for the progress of decidualization. Cystatin B (Cstb) is highly expressed in the decidual tissue of the uterus, but its regulation and mechanism in decidualization are not known.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Ida Marie Boisen, Anders Rehfeld, Iris Mos, Nadia Nicholine Poulsen, John Erik Nielsen, Peter Schwarz, Lars Rejnmark, Steen Dissing, Pernille Bach-Mortensen, Anders Juul, Hans Brauner-Osborne, Beate Lanske, Martin Blomberg Jensen
Summary: This study elucidates the important role of CaSR in human sperm, particularly in sensing calcium, magnesium, and bicarbonate ions, and participating in sperm activation and the acrosome reaction.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Endocrinology & Metabolism
Dian A. Teguh, Jordan L. Nustad, Amanda E. Craven, Daniel J. Brooks, Heike Arlt, Dorothy Hu, Roland Baron, Beate Lanske, Mary L. Bouxsein
Summary: The study showed that Abaloparatide (ABL) can increase bone density and strength in rats, while promoting bone formation and having limited effects on bone resorption.
Article
Endocrinology & Metabolism
Phuong T. Le, Hanghang Liu, Lama Alabdulaaly, Yosta Vegting, Isabella L. Calle, Francesca Gori, Beate Lanske, Roland Baron, Clifford J. Rosen
Summary: Conditional deletion of the PTH receptor in mesenchymal progenitors leads to a reduction in osteoblast differentiation and bone mass, while enhancing adipogenesis and bone marrow adipose tissue. The deficiency of Pth1r in mesenchymal progenitors increases the expression of the pro-adipogenic zinc finger transcription factor Zfp467. Genetic loss of Zfp467 results in a shift towards osteogenesis and increased bone mass.
Article
Endocrinology & Metabolism
Leah Hawkins Bressler, Marc A. Fritz, San-Pin Wu, Lingwen Yuan, Suzanna Kafer, Tianyuan Wang, Francesco J. DeMayo, Steven L. Young
Summary: The study found reduced proliferation and angiogenesis markers, increased inflammation, and altered ER expression patterns in women with suboptimal endometrial thickening after clomiphene treatment. The results suggest potential mechanisms underlying the lower pregnancy rates associated with suboptimal endometrial thickening in these women.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Multidisciplinary Sciences
Andrew M. Kelleher, Rohit Setlem, Francoise Dantzer, Francesco J. DeMayo, John P. Lydon, W. Lee Kraus
Summary: This study found that catalytic inhibition or genetic ablation of PARP-1 and PARP-2 in the uterus led to pregnancy loss in mice, associated with increased p53 signaling and an increased population of senescent decidual cells, with decidualization failure being the cause of pregnancy loss.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Manoj K. Madhavan, Francesco J. DeMayo, John P. Lydon, Niraj R. Joshi, Asgerally T. Fazleabas, Ripla Arora
Summary: The uterine luminal epithelium folds characteristically in mammals, including humans, horses and rodents. However, the precise function of these folds remains unknown. This study reveals dynamic changes in the 3D uterine folding pattern during early pregnancy and suggests that the folds and chambers are two distinct structures with specific roles in implantation and post-implantation morphogenesis.
Article
Endocrinology & Metabolism
Eileen J. Daley, Sung-Hee Yoon, Monica Reyes, Michael Bruce, Daniel J. Brooks, Mary Bouxsein, John T. Potts, Henry M. Kronenberg, Marc N. Wein, Beate Lanske, Harald Juppner, Thomas J. Gardella
Summary: Rodent models are commonly used for evaluating pharmacologic activities and therapeutic utility of PTH and PTHrP ligands and analogues. However, the differences in amino acid sequences between rodent and human PTH receptors can lead to variations in receptor-binding and signaling potencies for these ligands. To overcome this uncertainty, a humanized mouse model was created by replacing a segment of the murine PTH1R gene with the human PTH1R gene. This model showed functional responses consistent with a fully functional human PTH1R protein.
Article
Developmental Biology
Mackenzie J. Dickson, Yeong Seok Oh, Artiom Gruzdev, Rong Li, Nuria Balaguer, Andrew M. Kelleher, Thomas E. Spencer, San-Pin Wu, Francesco J. DeMayo
Summary: Approximately 75% of unsuccessful pregnancies are caused by implantation failure. This study developed a mouse strain with a decidual-specific iCre recombinase and confirmed its activity and tissue specificity during pregnancy. This research provides a valuable tool for investigating genetic networks and cellular dynamics associated with decidualization and infertility.
Article
Medicine, General & Internal
Joseph Wider, Vishnu V. R. Undyala, Beate Lanske, Nabanita S. Datta, Karin Przyklenk
Summary: Research has shown that PTHrP and abaloparatide have cardioprotective effects in myocardial ischemia-reperfusion injury, reducing cardiac cell death and infarct size.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Endocrinology & Metabolism
Sylvia C. Hewitt, San-pin Wu, Tianyuan Wang, Madhumita Ray, Marja Brolinson, Steven L. Young, Thomas E. Spencer, Alan DeCherney, Francesco J. DeMayo
Summary: This study investigates the molecular processes underlying estrogen responses and their effects on endometrial health. By analyzing the ESR1 cistromes and transcriptomes of endometrial biopsies and organoids, the study identifies candidate ESR1 target genes and reveals differences in the distribution and regulation of ESR1 binding sites between different stages of the menstrual cycle. The findings provide valuable resources for understanding how estrogen affects endometrial function and health.
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact via Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of- function CTCF mutation indicates its importance in uterine homeostasis. Deleting Ctcf gene in female reproductive tissues of mice resulted in reduced uterine glands and hypoplastic uteri with decreased endometrial stroma. Ctcf deletion also altered the expression of progesterone-responsive genes, including Ihh, Fst, and Errfi1. Our findings highlight the key role of uterine Ctcf in progesterone-dependent expression and optimal post-pubertal uterine development.
Article
Multidisciplinary Sciences
Rong Li, Tianyuan Wang, Ryan M. Marquardt, John P. Lydon, San-Pin Wu, Francesco J. DeMayo
Summary: Estrogen and progesterone regulate uterine biology through their receptors ERα and PR, respectively. This study identifies TRIM28 as a protein that interacts with ERα and PR to regulate uterine function. TRIM28 deficiency disrupts PR and ERα chromatin binding, resulting in the inability of the uterus to support early pregnancy.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact through Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of-function CTCF mutation in endometrial tumors indicates its importance in uterine homeostasis. Our study reveals that uterine Ctcf plays a key role in progesterone-dependent expression of uterine genes underlying optimal post-pubertal uterine development.
Article
Endocrinology & Metabolism
Jun Huang, Sohana Siyar, Rita Sharma, Isabella Herrig, Lauren Wise, Spencer Aidt, Edward List, John J. Kopchick, Vishwajeet Puri, Kevin Y. Lee
Summary: Growth hormone stimulates lipolysis by reducing the expression of FSP27 in adipose tissue. A subtype of adipocytes, termed type 2 adipocytes, shows increased GH-induced signaling and lipolysis compared to other subtypes. Type 2 adipocytes play a critical role in GH-mediated lipolysis and metabolic response.
Article
Multidisciplinary Sciences
San-Pin Wu, Tianyuan Wang, Zheng-Chen Yao, Mary C. Peavey, Xilong Li, Lecong Zhou, Irina V. Larina, Francesco J. DeMayo
Summary: PGR plays a pivotal role in directing the molecular program for uterine remodeling and pregnancy support. Mutant PGR can lead to dysfunction of the myometrium, affecting fertility and pregnancy adaptation.