Review
Physiology
Evelyn E. Telfer, Johanne Grosbois, Yvonne L. Odey, Roseanne Rosario, Richard A. Anderson
Summary: This review discusses the mechanisms and regulatory pathways that govern the processes of oocyte and follicle formation, growth, and ovulation, with a focus on human oocytes/follicles. It emphasizes the effects of aging on oocyte number and quality, as well as the clinical implications. Additionally, it outlines the current developments in culture systems for follicle growth and the emerging prospects for generating new oocytes from stem cells.
PHYSIOLOGICAL REVIEWS
(2023)
Article
Cell Biology
Atsuko Kusuhara, Elnur Babayev, Luhan T. Zhou, Vijay P. Singh, Jennifer L. Gerton, Francesca E. Duncan
Summary: The study found that egg quality parameters in mice improve during the pubertal transition, but eggs in the early pubertal period exhibit chromosome configuration abnormalities, indicating a cell cycle delay and originating from more immature follicles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Igor Astudillo, Karla Aspee, Jaime Palomino, Oscar A. Peralta, Victor H. Parraguez, Monica de los Reyes
Summary: Mammalian ovaries typically contain one oocyte in each follicle, but poly-ovular follicles with more than one oocyte are observed in some species, especially canines. Oocytes from poly-ovular follicles have different morphologies and meiotic potentials, which affects their in vitro maturation. Evaluating the maturity potential of oocytes according to follicle type and estrous cycle is crucial for improving in vitro maturation and reproductive biotechnologies in canines. This study found that oocytes from poly-ovular follicles have slower meiotic development compared to those from mono-ovular follicles, but in vitro maturation is still possible.
Review
Cell Biology
A. Yu. Kulibin, E. A. Malolina
Summary: Although a complete reconstruction of spermatogenesis in vitro under fully defined conditions has not been achieved, many techniques have been proposed to get closer to that aim. The most successful technique is the organ culture method, which can produce functional haploid cells. However, this method is based on culturing intact testis tissue with unknown factors. Different types of 3D cultures have been discussed where specific testicular cell populations may be aggregated and the impact of each cell population may be examined. Unfortunately, germ cell development does not proceed further than the pachytene stage of meiosis there, with rare exceptions. Finally, recent studies focusing on germ cells in a conventional adherent cell culture have explored issues with in vitro meiosis and provided insight into the mechanisms of meiotic initiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Ana Filipa Ferreira, Juliana Machado-Simoes, Maria Soares, Ana Paula Sousa, Joao Ramalho-Santos, Teresa Almeida-Santos
Summary: Sirtuins play an important role in mammalian reproductive processes, including folliculogenesis and oocyte maturation. However, the exact mechanisms of sirtuin function are still unclear, and there is limited information on the subcellular localization of sirtuins during bovine meiosis.
Article
Cell Biology
Juan Ge, Na Zhang, Shoubin Tang, Feifei Hu, Xiaojing Hou, Hongzheng Sun, Longsen Han, Qiang Wang
Summary: Maternal diabetes impairs oocyte quality, but controlling the PDK1-mediated Ser232-PDHE1α phosphorylation pathway can partly protect oocytes from the effects of diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lei Peng, Yijing He, Weihan Wang, Yajie Chu, Qixin Lin, Rong Rui, Qiao Li, Shiqiang Ju
Summary: In this study, the importance of PAK1 in regulating spindle assembly and cell cycle progression during the first meiotic division of porcine oocytes was highlighted. The activated PAK1 showed dynamic expression and its subcellular localization was closely associated with spindle dynamics. Inhibition of PAK1 activity by IPA-3 led to impaired extrusion of PB1 and failure of bipolar spindle formation, which could be restored by reducing disulfide bonds between PAK1 and IPA-3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Du Xiang, Yang Liu, Encheng Zhou, Yanfeng Wang
Summary: This article discusses the gradual decrease in ovarian reserve with age, the related diseases that may occur after the loss of estrogen protection postmenopausally, as well as the controversy surrounding PHRT and AO technology. The article also compares the advantages and disadvantages of different polymer biomaterials for use in follicle IVC, providing a theoretical basis for the development of mature AO technology.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jodi D. Weiss, Shelby L. McVey, Sarah E. Stinebaugh, Caroline F. Sullivan, R. Kelly Dawe, Natalie J. Nannas
Summary: The assembly and disassembly of spindles in maize meiosis exhibit variability but follow a predictable series of events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mengxue Zheng, Jesus Cadenas, Susanne Elisabeth Pors, Tasnim Esa, Stine Gry Kristensen, Linn Salto Mamsen, Cristina Subiran Adrados, Claus Yding Andersen
Summary: This study aimed to optimize the culture conditions for murine preantral follicles to improve their growth and survival. The results showed that altering the alginate concentration, adding estradiol, and increasing follicle-stimulating hormone concentration can promote the growth and development of preantral follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Chong Wang, Shuying Qu, Jie Zhang, Ming Fu, Xiaofei Chen, Wanqi Liang
Summary: Meiotic recombination begins with the formation of double-strand breaks in DNA, which is catalyzed by SPO11 and accessory proteins like MEI4/PRD2. In rice, the MEI4/PRD2 homolog OsPRD2 plays a crucial role in meiosis, primarily involved in the generation of chromosomes and assembly of the spindle. Although OsPRD2 does not participate in spindle assembly in rice, it plays a conserved role in the formation of double-strand breaks in DNA.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Reproductive Biology
Jing Xu, Mary B. Zelinski
Summary: In vitro follicle development (IVFD) is a valuable model for understanding folliculogenesis, screening ovarian toxicity, and preserving fertility and species. This review discusses the culture systems used in IVFD across various species and the improvements made in follicle growth and oocyte maturation through optimization of culture conditions. The evaluation of oocyte quality following IVFD, including epigenetic and genetic profile, maturation, embryonic development, and pregnancy outcomes, is summarized. The review also highlights the limitations and knowledge gaps in IVFD for proper oocyte development. This information contributes to the goal of producing competent oocytes with genetic integrity for successful embryonic development and healthy offspring.
BIOLOGY OF REPRODUCTION
(2022)
Article
Plant Sciences
Xuhong Zhou, Shuying Li, Xiaomi Yang
Summary: In this study, the researchers investigated the mechanisms of meiosis in carnation and found that DcPS1 and OSDLa play important roles in diplogamete production. The interaction between OSDLa and DcPS1 and DcRAD51D was also discovered. These findings shed light on the understanding of meiosis and provide a new pathway for further meiosis-related studies.
Article
Dentistry, Oral Surgery & Medicine
William Minston, Daniel Benchimol, Reinhilde Jacobs, Bodil Lund, Carina Kruger Weiner, Wim Coucke, Xie-Qi Shi
Summary: This study found that the odds ratio for success was 11 times higher in patients with painful DDwR compared to SA (p = 0.03), and 25.9 times higher when considering solely objective outcome (p = 0.03). In the absence of subchondral pseudocyst, there were 5.2 times higher odds to have a successful subjective outcome (p = 0.04). Extensive bone apposition on the temporal joint component indicated a 9.3 times higher likelihood of a failed objective outcome (p = 0.04).
Article
Medical Laboratory Technology
Wim Coucke, Mohamed Rida Soumali, Peter Graham, Samantha Shepherd, Tony Badrick
Summary: This study proposes a framework for quantitatively evaluating the impact of adding more EQA rounds or samples. The results show that although correlation leads to the negation of some samples, the number of negated samples does not exceed 50% of the total number of samples. Furthermore, there is a positive correlation between correlation and EQA results.
CLINICA CHIMICA ACTA
(2022)
Article
Medical Laboratory Technology
Christoph Buchta, Wim Coucke, Wolfgang Huf, Andrea Griesmacher, Mathias M. Mueller, Wolfgang R. Mayr, Oystein Flesland, Constantina Politis, Johanna Wiersum-Osselton, Andres Aburto, Tony Badrick, Lobna Bouacida, Marek Budina, Joseph A. Duenas, Wolf-Jochen Geilenkeuser, Andre V. P. Guimaraes, Ana Hecimovic, Markus Jutzi, Chang-Keun Lee, Young Ae Lim, Joy Mammen, Petra Magdolna Molnar, Azita Mokhtari, Giuseppa Morabito, Eduardo Muniz-Diaz, Truscha Niekerk, Anja Pakkanen, Paola Pezzati, Razvan Popa, Erika Sarkany, Jean-Pascal Siest, Dhitiwass Suvagandha, Marc Thelen, Jenny Ullhagen, Dalius Vitkus, Guenther F. Koermoeczi
Summary: There is no direct association between national regulatory and economic conditions and the quality performance of medical laboratories in immunohaematology. However, the support provided by EQA providers in case of incorrect results may be associated with lower error rates.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Luc Vrielinck, Catalina Moreno-Rabie, Serge Schepers, Peter Van Eyken, Wim Coucke, Constantinus Politis
Summary: This retrospective cohort study aimed to investigate the occurrence of peri-zygomatic infection (PZI) as a complication following zygomatic implant (ZI) placement over a period of 22 years. Among 302 eligible patients, 45 cases were associated with the development of PZI. The study found that PZI can lead to zygomatic implant failure and should be informed to patients as a potential complication.
CLINICAL ORAL IMPLANTS RESEARCH
(2022)
Article
Obstetrics & Gynecology
Shari Mackens, Panagiotis Drakopoulos, Margot Fauve Moeykens, Linde Mostinckx, Liese Boudry, Ingrid Segers, Herman Tournaye, Christophe Blockeel, Michel De Vos
Summary: This study aimed to investigate whether there are differences in cumulative live birth rates (CLBR) between different phenotypes of polycystic ovary syndrome (PCOS) after a freeze-all strategy is used to prevent OHSS. The results showed that the CLBR in women with PCOS was around 70% regardless of phenotype when a freeze-all strategy was used.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Ricassio S. Barberino, Thae Lanne B. G. Lins, Alane P. O. Monte, Bruna B. Gouveia, Daniela S. P. Campinho, Raimundo C. Palheta, Johan E. J. Smitz, Maria Helena T. Matos
Summary: This study evaluated the protective effect of melatonin before cyclophosphamide administration on ovarian function in mice. Pre-treatment with 20 mg/kg melatonin showed reductions in primordial follicle loss, apoptosis, and oxidative damage, as well as an increase in GSH concentrations. Melatonin exerted its protective effects through the MT1 receptor and PTEN/Akt/FOXO3a signaling pathway.
REPRODUCTIVE SCIENCES
(2022)
Article
Genetics & Heredity
Ileana Mateizel, Samuel Santos-Ribeiro, Ingrid Segers, Koen Wouters, Shari Mackens, Greta Verheyen
Summary: The study results indicate that the use of CultActive does not improve embryo development outcomes in patients with a history of embryo developmental problems.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Letter
Genetics & Heredity
Ileana Mateizel, Samuel Santos-Ribeiro, Ingrid Segers, Koen Wouters, Shari Mackens, Greta Verheyen
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Reproductive Biology
Anamaria-Cristina Herta, Lucia Mengden, Nazli Akin, Katy Billooye, Wim Coucke, Julia Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: This study explored the glucose-related pathways in different follicle cell types and found altered metabolic patterns in in vitro-cultured mouse follicles, suggesting potential impacts on oocyte competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Medical Laboratory Technology
Wim Coucke, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove, Jan Tytgat, Andre Vanescote, Alain G. Verstraete, Sarah Wille, Arnaud Capron
Summary: This study evaluates the performance of automated systems in ethanol concentration analysis. The results show that Alinity c performs poorly in terms of bias and measurement uncertainty, while Architect and Cobas c501/c502 systems have the lowest variability.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ricassio S. Barberino, Tais J. S. Macedo, Thae Lanne B. G. Lins, Vanuzia G. Menezes, Regina L. S. Silva, Alane P. O. Monte, Raimundo C. Palheta, Johan E. J. Smitz, Maria Helena T. Matos
Summary: This study investigated the expression of melatonin receptor type 1 (MT1) protein in sheep ovaries and evaluated the effects of melatonin on the survival and development of primordial follicles after in vitro culture. The results showed that 100 pg/ml melatonin could increase follicular survival and activation. Inhibition of the PI3K pathway resulted in increased apoptosis and decreased activation of primordial follicles. These findings suggest that 100 pg/ml melatonin can promote the survival and activation of primordial follicles through the PI3K signaling pathway in sheep ovarian tissue culture.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Obstetrics & Gynecology
Lucia von Mengden, Marco Antonio De Bastiani, Lucas Kich Grun, Florencia Barbe-Tuana, Tom Adriaenssens, Johan Smitz, Leticia Schmidt Arruda, Carlos Alberto Link, Fabio Klamt
Summary: Metadata analysis of public microarray datasets using bioinformatics tools has been successfully used in several biomedical fields in the search for biomarkers. In reproductive science, the analysis of gene expression datasets from human cumulus cells has revealed potential biomarkers for oocyte quality, which were validated with clinical samples. This study emphasizes the importance of considering patients' clinical characteristics when selecting relevant biomarkers for use in the clinical setting.
REPRODUCTIVE SCIENCES
(2023)
Review
Obstetrics & Gynecology
Annelore Van Der Kelen, Ozlem Okutman, Elodie Javey, Munevver Serdarogullari, Charlotte Janssens, Manjusha S. Ghosh, Bart J. H. Dequeker, Florence Perold, Claire Kastner, Emmanuelle Kieffer, Ingrid Segers, Alexander Gheldof, Frederik J. Hes, Karen Sermon, Willem Verpoest, Stephane Viville
Summary: High-throughput sequencing methods have led to the identification of numerous gene variants in male and female infertility. Evidence-based curation of these genes can improve diagnostic performance and genetic research.
HUMAN REPRODUCTION UPDATE
(2023)
Article
Obstetrics & Gynecology
Johan Sterckx, Koen Wouters, Ileana Mateizel, Ingrid Segers, Anick De Vos, Lisbet Van Landuyt, Hilde van de Velde, Herman Tournaye, Neelke De Munck
Summary: Based on a 10-year evaluation of an electronic witnessing system, it was found that when applied correctly, the system can replace manual witnessing to prevent sample mix-up. The overall mismatch and administrator assign rates remained stable during the evaluated period. The system needs to be used in combination with manual labeling to ensure correct sample assignment.
HUMAN REPRODUCTION
(2023)
Article
Multidisciplinary Sciences
Catalina Moreno-Rabie, Laurence Lapauw, Hugo Gaeta-Araujo, Andre Ferreira-Leite, Wim Coucke, Tim van den Wyngaert, Reinhilde Jacobs
Summary: This study aimed to identify radiographic predictors of MRONJ in panoramic images of oncologic patients undergoing tooth extraction. The results showed that longer ARD treatment, teeth with absent or incomplete endodontic fillings, presence of caries, widened periodontal ligament space and/or periapical lesions, as well as sclerotic and heterogenous bone patterns were associated with a higher risk of MRONJ development.
SCIENTIFIC REPORTS
(2022)
