Article
Biochemistry & Molecular Biology
Stefany Castaldi, Marco Masi, Francisco Sautua, Alessio Cimmino, Rachele Isticato, Marcelo Carmona, Angela Tuzi, Antonio Evidente
Summary: This study identified natural compounds in Pseudomonas fluorescens 9 with strong antifungal activity, especially against the soybean pathogen Macrophomina phaseolina. Compound 1 showed promising activity and could be considered for controlling soybean pathogens, suggesting further research on large-scale production and suitable formulations for greenhouse and field applications.
Article
Agronomy
Edelweiss Airam Rangel-Montoya, Carmen Sanjuana Delgado-Ramirez, Edgardo Sepulveda, Rufina Hernandez-Martinez
Summary: Two strains of B. amyloliquefaciens (BsA3MX and BsC11MX) were identified, which grew well under low pH and high salinity conditions, and inhibited the growth of M. phaseolina through the combined action of volatile and diffusible compounds. Additionally, they exhibited several beneficial traits, including the production of siderophores and indole-3-acetic acid, ACC-deaminase activity, phosphate and zinc solubilization, and reduction of microsclerotia germination. Greenhouse assays showed that the BsA3MX strain alleviated plant damage caused by M. phaseolina and promoted foliage and root growth.
Article
Agronomy
Josefina Viejobueno, Patricia Liliana Albornoz, Maria Camacho, Berta de los Santos, Martin Gustavo Martinez-Zamora, Sergio Miguel Salazar
Summary: This study evaluated the protective effect of A. brasilense strains isolated from different plants against the fungal pathogen Macrophomina phaseolina. Results showed that REC3 and 2A1 strains could induce defense response in strawberry plants, increasing tolerance to the disease.
Article
Horticulture
Josefina Viejobueno, Natalia Rodriguez-Berbel, Luis Miranda, Berta de los Santos, Maria Camacho
Summary: The study evaluated the efficacy of antagonistic bacteria in controlling charcoal rot in strawberries, identifying the Brevibacterium sp. Hvs8 strain as a potential candidate. The Hvs8 strain demonstrated reduced disease incidence and severity, as well as decreased plant mortality associated with Macrophomina phaseolina in both greenhouse and field conditions.
Article
Agronomy
Gaber Attia Abo-Zaid, Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Soraya Abdel-Fattah Sabry
Summary: The secretion of siderophores by Pseudomonas aeruginosa F2 and P. fluorescens JY3 was evaluated and found to have an inhibitory effect on Fusarium oxysporum and Rhizoctonia solani. Two optimized media were used to produce siderophores as biocontrol agents, and the cell-free supernatants containing siderophores were used to prepare bio-friendly formulations. These formulations showed significant reduction in damping-off caused by F. oxysporum and R. solani, as well as promoting the growth of wheat plants.
Article
Biology
Alba Pacheco-Moreno, Francesca L. Stefanato, Jonathan J. Ford, Christine Trippel, Simon Uszkoreit, Laura Ferrafiat, Lucia Grenga, Ruth Dickens, Nathan Kelly, Alexander D. H. Kingdon, Liana Ambrosetti, Sergey A. Nepogodiev, Kim C. Findlay, Jitender Cheema, Martin Trick, Govind Chandra, Graham Tomalin, Jacob G. Malone, Andrew W. Truman
Summary: Researchers genetically and phenotypically characterized the Pseudomonas fluorescens population in a commercial potato field, revealing strong correlations between specialized metabolite biosynthesis and antagonism of potato pathogens. They found that specialized metabolites such as hydrogen cyanide and cyclic lipopeptides play key roles in inhibiting the pathogens, and that the population dynamics of Pseudomonas bacteria in the field are influenced by plant colonization and environmental factors.
Article
Biotechnology & Applied Microbiology
Dinorah Anabella Sarli, Gisele Teixeira Gomes, Sebastian Reznikov, Federico Francisco Acosta, Julia Ines Farina, Osvaldo Daniel Delgado, Leandro A. Sanchez
Summary: The aim of this study was to characterize cold-adapted bacteria by testing their plant growth promotion (PGP) features and antagonistic activity against Macrophomina phaseolina, both in vitro and on soybean seeds. The results showed that Serratia proteamaculans 136, 137, and Burkholderia gladioli MB39 exhibited PGP features and inhibition of M. phaseolina infection by producing different antifungal compounds. These findings highlight the potential of these bacteria as candidates for developing microbial formulations to promote plant growth and provide antifungal protection in soybean crops.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agronomy
D. T. Nagrale, S. P. Gawande, Vivek Shah, Pooja Verma, N. S. Hiremani, T. Prabhulinga, Nandini Gokte-Narkhedkar, V. N. Waghmare
Summary: Rhizospheric bacteria produce microbial volatile organic compounds (mVOCs) that can be used as biocontrol components against phytopathogenic fungi. This study identified mVOCs produced by cotton rhizobacteria and demonstrated their biocontrol potential against Macrophomina phaseolina fungus.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Immunology
A. P. Sridharan, Sugitha Thangappan, G. Karthikeyan, S. Nakkeeran, Sivakumar Uthandi
Summary: The endophytic fungal antagonist Trichoderma longibrachiatum EF5 showed biocontrol activity against the soilborne fungal pathogen Macrophomina phaseolina, with metabolites extracted from EF5 exhibiting significant inhibition. Dual culture of EF5 and M. phaseolina displayed entangled mycelial structures and hindered hyphal growth. Metabolites identified through GC-MS analysis revealed unique compounds in EF5 with potential plant growth promotion and antimicrobial properties, suggesting its potential as a biocontrol agent.
MICROBIAL PATHOGENESIS
(2021)
Article
Food Science & Technology
Mohamed S. Sheteiwy, Ahmed M. El-Sawah, Yoshihiro Kobae, Farwa Basit, Paul Holford, Haishui Yang, Ali El-Keblawy, Ghada G. Abdel-Fattah, Shucai Wang, Jose Luis Araus, Shereen Magdy Korany, Emad A. Alsherif, Hamada AbdElgawad
Summary: This study aimed to evaluate the individual and combined effects of arbuscular mycorrhizal fungi (AMF) and Bradyrhizobium on the growth, yield, and nutritional quality of guar plants. The results showed that inoculation with AMF and/or Bradyrhizobium improved the chemical composition and morpho-physiological traits of guar seeds and leaves. Furthermore, the inoculation increased the mineral, fiber, lipid, crude protein, and ash contents of guar, as well as the production of sugars, organic acids, amino acids, and fatty acids. This study sheds light on how AMF and Bradyrhizobium enhance guar yield and metabolism, and suggests that their combined inoculation is an innovative approach to improve the growth, yield, and quality of guar.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Qingqing Yang, Qing Yan, Bo Zhang, Li-qun Zhang, Xiaogang Wu
Summary: Carbon metabolism plays a critical role in the production of the bacterial secondary metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) and the biocontrol capacity of Pseudomonas fluorescens 2P24. The citrate synthase gene gltA regulates the expression of 2,4-DAPG biosynthesis genes and is essential for biocontrol activity. Mutation of gltA decreases 2,4-DAPG synthesis and impairs biocontrol capacity against bacterial wilt disease, while citrate addition enhances 2,4-DAPG production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Vivek H. Khambhati, Hamed K. Abbas, Michael Sulyok, Maria Tomaso-Peterson, Jian Chen, Wayne Thomas Shier
Summary: Macrophomina phaseolina (Mp) is a fungal pathogen that enters host roots by releasing toxins, and it has been found to produce several potent phytotoxins. A previous study discovered 14 previously unreported secondary metabolites in Mp isolates from soybean plants, including mellein. This study investigated the production of mellein by Mp isolates and its role in phytotoxicity, finding that mellein concentrations had weak correlations with phytotoxic effects in soybean seedlings.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Stefany Castaldi, Claudia Petrillo, Giuliana Donadio, Fabrizio Dal Piaz, Alessio Cimmino, Marco Masi, Antonio Evidente, Rachele Isticato
Summary: In recent years, there has been a global push to find eco-friendly and safe strategies to maintain agricultural productivity, amid concerns over the excessive use of pesticides and fertilizers. Among alternative approaches, Plant Growth-Promoting (PGP) rhizobacteria are gaining attention as biocontrol agents against agricultural pests. A study focused on the salt-pan rhizobacteria identified Bacillus vallismortis strain RHFS10 as a potential alternative to chemical pesticides in efficiently controlling the phytopathogenic fungus M. phaseolina, with lower and more stable antifungal activity compared to a commercial fungicide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agricultural Engineering
Yuwen Luo, Luo Luo, Xuejiao Huang, Daihua Jiang, Xiaogang Wu, Zhenlun Li
Summary: The ammonium and nitrate removal performance and metabolic pathways of Pseudomonas fluorescens 2P24 were investigated. Strain 2P24 was able to completely remove 100 mg/L of ammonium and nitrate with removal rates of 8.27 mg/L/h and 4.29 mg/L/h, respectively. The study revealed that most of the ammonium and nitrate were converted to biological nitrogen via assimilation, and only small amounts of nitrous oxide were released.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agronomy
Kamal A. M. Abo-Elyousr, Maged M. Saad, Adel D. Al-Qurashi, Omer H. M. Ibrahim, Magdi A. A. Mousa
Summary: Brevibacillus parabrevis and Pseudomonas fluorescens show potential as biocontrol agents against Fusarium wilt in cumin.
