Journal
BIOLOGICALS
Volume 40, Issue 1, Pages 84-87Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biologicals.2011.11.004
Keywords
Test development; ELISA; Vaccine; Poliovirus; D-antigen; IPV
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A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol was optimized by minimizing the total incubation time to 1 h, and by replacing the signal reagent 3,3',5,5'-tetramethylbenzidine by a chemiluminogenic signal reagent with a theoretical low intrinsic background and high dynamic range. (C) 2011 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
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