Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 147, Issue 1-3, Pages 246-250Publisher
HUMANA PRESS INC
DOI: 10.1007/s12011-011-9307-3
Keywords
Aluminum; Immune functions; Splenic lymphocytes; Rats
Funding
- National Natural Science Fund of China [31172375]
- Heilongjiang Educational Bureau [12511028]
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The effects of Aluminum (Al) exposure on immune functions of cultured splenic T and B lymphocytes of rats were studied. The lymphocytes were isolated from spleen of healthy male Wistar rats weighing 110-120 g. The cultured cells in RPMI-1640 medium were exposed to 0 (control group), 0.035 (low-dose group), 0.07 (medial-dose group), and 0.14 (high-dose group) mg/mL Al3+ as aluminum trichloride (AlCl3) in an incubator under 5% CO2 at 37A degrees C for 24 h. The T and B lymphocyte proliferation was measured with a tetrazolium dye colorimetric assay. The levels of interleukin (IL)-2, IL-6, and tumor necrosis factor (TNF)-alpha were determined by iodine [I-125] IL-2, IL-6, and TNF-alpha radioimmunoassay kits, respectively. The proportions of CD3(+), CD4(+), and CD8(+) T lymphocytes were measured with a flow cytometer. The results showed that the T and B lymphocyte proliferation, the levels of IL-2, IL-6, TNF-alpha, the proportions of CD3(+) and CD4(+) T lymphocytes, and the ratio of CD4(+)/CD8(+) T lymphocytes were lowered by Al treatments, while the proportion of CD8(+) T lymphocytes was increased. These findings indicate that Al exposure can inhibit the immune functions of splenic T and B lymphocytes of rats in vitro.
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