Article
Biochemistry & Molecular Biology
Veer S. Bhatt, Robert Ashley, Anna Sundborger-Lunna
Summary: This study reveals that endophilin B1 assembles into helical scaffolds on membranes driven by interactions between N-terminal helix H0 and the lipid bilayer. The lipid composition plays a role in dictating endophilin B1 activity.
Article
Biochemistry & Molecular Biology
Benjamin Kroppen, Nelli Teske, King F. Yambire, Niels Denkert, Indrani Mukherjee, Daryna Tarasenko, Garima Jaipuria, Markus Zweckstetter, Ira Milosevic, Claudia Steinem, Michael Meinecke
Summary: The membrane binding and curvature-inducing ENTH domain of epsin1 is regulated by phosphatidylserine (PS), with membrane composition playing an important role in protein-dependent membrane deformation during clathrin-mediated endocytosis. ENTH forms rigid homo-oligomers on PS-containing membranes, which are necessary for inducing membrane curvature. Oligomerization mutants can bind to membranes but lack membrane remodeling activity, affecting the ability to rescue defects in EGFR endocytosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Nathan M. Willy, Federico Colombo, Scott Huber, Anna C. Smith, Erienne G. Norton, Comert Kural, Emanuele Cocucci
Summary: The research demonstrates the important role of CALM in the formation of clathrin-coated vesicles, showing that an increase in its level promotes vesicle completion. Variations in adaptor protein expression levels among different cells support the hypothesis that the efficiency of clathrin-mediated endocytosis is tissue-specific.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Camille Lemaigre, Apolline Ceuppens, Cesar Augusto Valades-Cruz, Benjamin Ledoux, Bastien Vanbeneden, Mujtaba Hassan, Fredrik R. Zetterberg, Ulf J. Nilsson, Ludger Johannes, Christian Wunder, Henri-Francois Renard, Pierre Morsomme
Summary: This study investigates the endocytosis of L1CAM and its role in nervous system development, cancer development, and metastasis prognosis. It reveals that there are two isoforms of L1CAM that undergo endocytosis mediated by N-BAR and F-BAR domain proteins, as well as galectins. These findings are important for understanding the pathophysiological role of L1CAM.
Article
Biology
Daniel A. Phillips, Lori A. Zacharoff, Cheri M. Hampton, Grace W. Chong, Anthony P. Malanoski, Lauren Ann Metskas, Shuai Xu, Lina J. Bird, Brian J. Eddie, Aleksandr E. Miklos, Grant J. Jensen, Lawrence F. Drummy, Mohamed Y. El-Naggar, Sarah M. Glaven
Summary: BAR domain proteins are a superfamily of coiled-coil proteins that influence membrane curvature in eukaryotes, and a bacterial protein BdpA with BAR domain-like activity has been discovered to impact the formation of membrane vesicles and outer membrane extensions. BdpA is essential for the uniform size distribution of membrane vesicles and the scaffolding of consistent diameter and curvature in OMEs, influencing the morphology of these structures. Overexpression of BdpA can promote the formation of OMEs in bacterial species where it is not typically observed, suggesting a new class of bacterial BAR domain-like proteins.
Article
Cell Biology
Irene Schiano Lomoriello, Sara Sigismund, Kasey J. Day
Summary: Endocytosis is a cellular mechanism for communication that involves internalization of molecules on the plasma membrane, modulating cell signaling and biological responses. This review focuses on recent advances in understanding the principles underlying endocytic vesicle formation, with an emphasis on liquid-liquid phase separation and membrane bending, and discusses how these properties affect receptor fate and signaling.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Binod Nepal, Aliasghar Sepehri, Themis Lazaridis
Summary: The ability of BAR domains to sculpt membranes is usually related to their intrinsic curvature, but their conformation does not always match the diameter of the generated membrane tubules. Higher oligomeric structures can influence membrane bending of I-BAR domains, even if they are unrelated to the intrinsic curvature of the dimer.
Article
Medicine, Research & Experimental
Rita C. Andersen, Jan H. Schmidt, Joscha Rombach, Matthew D. Lycas, Nikolaj R. Christensen, Viktor K. Lund, Donnie S. Stapleton, Signe S. Pedersen, Mathias A. Olsen, Mikkel Stoklund, Gith Noes-Holt, Tommas Te Nielsen, Mark P. Keller, Anna M. Jansen, Rasmus Herlo, Massimo Pietropaolo, Jens B. Simonsen, Ole Kjaerulff, Birgitte Holst, Alan D. Attie, Ulrik Gether, Kenneth L. Madsen
Summary: The BAR domain protein PICK1 plays a role in the formation and release of secretory granules, and coding variants associated with diabetes have been identified. The coding variants may cause dominant-negative effects by increasing the ability to induce membrane fission.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Shinya Yuge, Koichi Nishiyama, Yuichiro Arima, Yasuyuki Hanada, Eri Oguri-Nakamura, Sanshiro Hanada, Tomohiro Ishii, Yuki Wakayama, Urara Hasegawa, Kazuya Tsujita, Ryuji Yokokawa, Takashi Miura, Toshiki Itoh, Kenichi Tsujita, Naoki Mochizuki, Shigetomo Fukuhara
Summary: Blood flow-driven intraluminal pressure (IP) plays a crucial role in regulating wound angiogenesis. F-BAR proteins TOCA1 and CIP4 are key actin regulatory proteins that control endothelial cell migration and sense mechanical cell stretching to regulate wound angiogenesis.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Sarah R. Barger, Lauren Penfield, Shirin Bahmanyar
Summary: The nuclear envelope serves as a protective barrier for the cell's genome, undergoing dynamic remodeling processes essential for cell growth and maintenance. Recent studies have shown that lipids produced in the endoplasmic reticulum are utilized to remodel nuclear membranes, with lipid species and lipid-protein interactions playing crucial roles in controlling nuclear envelope dynamics. Coordination of lipid biosynthesis is important for ensuring genome protection through nuclear envelope remodeling.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Chemistry, Physical
Shivam Gupta, Taraknath Mandal
Summary: Recent experimental studies have found functional membrane microdomains (FMMs) in prokaryotic cells that are similar to lipid rafts in eukaryotic cells. This study used molecular dynamics simulations to investigate the mechanism and properties of domain formation in a model methicillin-resistant Staphylococcus aureus (MRSA) cell membrane. The results showed that domains were formed through the lateral segregation of staphyloxanthin (STX), which enhanced membrane integrity and attracted various membrane domain proteins.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Physical
Yida Zhang, Hong Zhang, Tongfei Zou, Meng Liu, Quanyi Wang, Yubing Pei, Yongjie Liu, Qingyuan Wang
Summary: In this study, a multiscale model was developed to couple a macro-model with physically based yield strength and a crystal plasticity model with micromechanical properties and realistic grain orientation. The simulation results showed that the microstructure had a significant impact on the macro-mechanical properties, and solid strengthening, grain boundaries, and dislocation density played crucial roles. The coupling simulation and microstructure evolution were successfully achieved using EBSD. The strain distribution was found to be closely related to the grain orientation, leading to inhomogeneous deformation and the formation of shear bands. This work provides guidance for structural integrity assessment and prediction of mechanical properties in engineering materials and components.
Article
Chemistry, Multidisciplinary
Mehdi Nikfar, Meghdad Razizadeh, Ratul Paul, Vladimir Muzykantov, Yaling Liu
Summary: Hitchhiking of nanocarriers on red blood cell membranes enhances the delivery of a wide range of nanoparticles, with studies exploring the impact of nanoparticle size, surface chemistry, and shear rate on the delivery process and biodistribution. Numerical simulations show that changing the interaction between nanoparticles and red blood cells can control the desorption process.
Review
Cell Biology
Christopher T. Lee, Matthew Akamatsu, Padmini Rangamani
Summary: Membrane budding and curvature generation are common features of many cellular trafficking processes, with clathrin-mediated endocytosis serving as a well-studied example. Recent advances in experimental methods have provided insights into the molecular mechanisms of membrane curvature generation, supported by computational models. Experimental perturbation of membranes lags behind protein studies in cells, highlighting the critical role of modeling in interpreting experimental measurements.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Engineering, Biomedical
Elizabeth Gacek, Ryan R. Mahutga, Victor H. Barocas
Summary: This study develops a discrete-continuum modeling scheme for analyzing tissue growth and remodeling, combining microstructural and cellular-scale models with macroscopic finite element models. The model is applied in case studies of arterial media, media and adventitia, and chronic dissection of an arterial wall, providing new data and insights into growth and remodeling processes.
ACTA BIOMATERIALIA
(2023)
Article
Cell Biology
Begona Ugarte-Uribe, Coline Prevost, Kushal Kumar Das, Patricia Bassereau, Ana J. Garcia-Saez
JOURNAL OF CELL SCIENCE
(2019)
Article
Cell Biology
Nicola De Franceschi, Maryam Alqabandi, Nolwenn Miguet, Christophe Caillat, Stephanie Mangenot, Winfried Weissenhorn, Patricia Bassereau
JOURNAL OF CELL SCIENCE
(2019)
Article
Cell Biology
Lucrecia Alberdi, Alexandra Vergnes, Jean-Baptiste Manneville, Dumizulu L. Tembo, Ziyan Fang, Yaya Zhao, Nina Schroeder, Audrey Dumont, Margaux Lagier, Patricia Bassereau, Lorena Redondo-Morata, Jean-Pierre Gorvel, Stephane Meresse
JOURNAL OF CELL SCIENCE
(2020)
Article
Multidisciplinary Sciences
Aurelie Bertin, Nicola de Franceschi, Eugenio de la Mora, Sourav Maity, Maryam Alqabandi, Nolwen Miguet, Aurelie di Cicco, Wouter H. Roos, Stephanie Mangenot, Winfried Weissenhorn, Patricia Bassereau
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Julien Pernier, Antoine Morchain, Valentina Caorsi, Aurelie Bertin, Hugo Bousquet, Patricia Bassereau, Evelyne Coudrier
JOURNAL OF CELL SCIENCE
(2020)
Article
Biophysics
Zack Jarin, Alexander J. Pak, Patricia Bassereau, Gregory A. Voth
Summary: This study reveals the significant roles of protein curvature and lipid clustering in the relaxation of large membrane deformations. Lipid clusters mediate interactions between I-BAR domains and cause the formation of aggregates, leading to more cylindrical protrusions within membrane protrusions compared to irregularly shaped ones.
BIOPHYSICAL JOURNAL
(2021)
Article
Biophysics
Alexandru Paraschiv, Thibaut J. Lagny, Christian Vanhille Campos, Evelyne Coudrier, Patricia Bassereau, Andela Saric
Summary: The study reveals that the protein linkers between the cell membrane and the membrane-cortex attachments play a significant role in the mechanical properties and reshaping ability of the cell membrane. The density of membrane-cortex attachments has a nonlinear impact on the force for tube extrusion, with higher concentrations of linkers providing increased stability against coalescence for membrane tubes. Experimental findings show that overexpression of Ezrin enhances extrusion force, while Ezrin depletion has minimal effect on the force.
BIOPHYSICAL JOURNAL
(2021)
Article
Biology
Kaushik Inamdar, Feng-Ching Tsai, Rayane Dibsy, Aurore de Poret, John Manzi, Peggy Merida, Remi Muller, Pekka Lappalainen, Philippe Roingeard, Johnson Mak, Patricia Bassereau, Cyril Favard, Delphine Muriaux
Summary: The cellular I-BAR protein IRSp53 has been identified as a crucial factor in HIV-1 membrane curvature during particle formation, showing a strong interplay with the retroviral Gag protein. IRSp53 is required for the progression of HIV-1 membrane curvature and its presence is observed in purified HIV-1 particles. The preferential localization of IRSp53 around HIV-1 Gag assembly sites highlights its importance in full HIV-1 particle assembly.
Article
Biology
R. Sikora, P. Bun, L. Danglot, M. Alqabandi, P. Bassereau, F. Niedergang, T. Galli, A. Zahraoui
Summary: Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. MICAL-L1 is found to be associated with tubulo-vesicular structures and to have a significant colocalization with markers of the Golgi apparatus and recycling endosomes, revealing a new role in cargo delivery to the plasma membrane.
Article
Biochemistry & Molecular Biology
Kimi Azad, Delphine Guilligay, Cecile Boscheron, Sourav Maity, Nicola De Franceschi, Guidenn Sulbaran, Gregory Effantin, Haiyan Wang, Jean-Philippe Kleman, Patricia Bassereau, Guy Schoehn, Wouter H. Roos, Ambroise Desfosses, Winfried Weissenhorn
Summary: This study presents cryo-EM structures of membrane-coated CHMP2A-CHMP3 filaments from Homo sapiens, which reveal helical filaments assembled by CHMP2A-CHMP3 heterodimers in the open ESCRT-III conformation. Inter-filament interactions are electrostatic, facilitating filament sliding upon VPS4-mediated polymer remodeling. Fluorescence microscopy and high-speed atomic force microscopy imaging confirm that VPS4 can constrict and cleave CHMP2A-CHMP3 membrane tubes. It is concluded that CHMP2A-CHMP3-VPS4 act as a minimal membrane fission machinery.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
J. Michael Henderson, Nina Ljubojevic, Sevan Belian, Thibault Chaze, Daryl Castaneda, Aude Battistella, Quentin Giai Gianetto, Mariette Matondo, Stephanie Descroix, Patricia Bassereau, Chiara Zurzolo
Summary: Tunnelling nanotubes (TNTs) are specialized actin-based protrusions that bridge distant cells to facilitate direct intercellular communication and cargo transfer in both physiological and pathological contexts. Linear actin filament elongation induced by Eps8 and IRSp53 is shown to promote functional and stable TNT formation. A micropatterning approach shows that TNTs originate via actin polymerization rather than cell dislodgement and exceed the length of conventional filopodia. Inhibition of Arp2/3 activity promotes functional TNT formation due to enhanced actin filament polymerization and elongation, suggesting a shift from branched to linear actin. An interaction between Eps8 and the I-BAR domain protein IRSp53 promotes formation of functional TNTs with an increased lifetime. Arp2/3 inhibition leads to reduction of Eps8 interactions with proteins facilitating actin filament depolymerization and turnover. Inhibition of branched actin networks reveals the role of linear actin filament polymerization in the formation of functional tunnelling nanotubes.
Article
Chemistry, Physical
Patricia Bassereau
Summary: This article is based on the concluding remarks lecture given at the Faraday Discussion meeting on peptide-membrane interactions, held online from September 8 to 10, 2021.
FARADAY DISCUSSIONS
(2021)
Review
Chemistry, Physical
Feng-Ching Tsai, Mijo Simunovic, Benoit Sorre, Aurelie Bertin, John Manzi, Andrew Callan-Jones, Patricia Bassereau
Summary: This review discusses two continuous models based on Helfrich elasticity energy to explain protein enrichment and curvature-driven protein sorting in cellular membranes. By analyzing experimental data of two BAR proteins, it examines the effectiveness of the spontaneous curvature model and the curvature mismatch model in explaining protein sorting.
Article
Biology
Nicola de Franceschi, Maryam Alqabandi, Winfried Weissenhorn, Patricia Bassereau
Article
Biology
Elena Beltran-Heredia, Feng-Ching Tsai, Samuel Salinas-Almaguer, Francisco J. Cao, Patricia Bassereau, Francisco Monroy
COMMUNICATIONS BIOLOGY
(2019)