Article
Biochemistry & Molecular Biology
Hao Chai, Qun Huang, Zixue Jiao, Shendong Wang, Chunguang Sun, Dechun Geng, Wei Xu
Summary: This study investigated the regulation of osteocyte-to-osteoblast differentiation by connexin 43 (Cx43). The results showed that calvarial osteolysis induced by titanium particles was partially attenuated in mice lacking Cx43. Silencing or overexpressing Cx43 affected beta-catenin expression, and Cx43 binding to beta-catenin inhibited the Wnt signaling pathway. These findings suggest that osteocytes may regulate osteoblast function through Cx43 during periprosthetic osteolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Alessandro Ianni, Michael Hofmann, Poonam Kumari, Shahriar Tarighi, Hamza M. Al-Tamari, Andre Goergens, Bernd Giebel, Hendrik Nolte, Marcus Krueger, Isabelle Salwig, Soni Savai Pullamsetti, Andreas Guenther, Andre Schneider, Thomas Braun
Summary: Idiopathic pulmonary fibrosis (IPF) is a highly aggressive and poorly understood lung disease, with lung epithelial cells playing a critical role in its initiation and progression. NUMB protein is necessary for the pathological activation of beta-catenin signaling in lung epithelial cells following bleomycin-induced injury. Depletion of Numb and Numblike reduces fibrotic lesion accumulation, preserves lung function, and increases survival rates in mice treated with bleomycin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Dong-Hoon Won, Da-Bin Hwang, Yoo-Sub Shin, Shin-Young Kim, Changuk Kim, In-Sun Hong, Byeong-Cheol Kang, Jeong-Hwan Che, Jun-Won Yun
Summary: This study investigated the effects of benzo[a]pyrene (BaP) on gap junctional intercellular communication (GJIC) and Wnt/beta-catenin signaling. BaP treatment inhibited GJIC and decreased the expression of connexin 43 (Cx43), a major GJIC protein. Treatment with the Wnt agonist CHIR99021 also inhibited GJIC and reduced Cx43 expression. It was found that Wnt/beta-catenin signaling negatively regulated GJIC.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Minghan Guan, Yifeng Huang, Xiaowen Lin
Summary: This study found that sufentanil can inhibit the proliferation, migration, invasion, and epithelial-mesenchymal transition of lung cancer cells by regulating the Wnt/beta-catenin signaling pathway.
Article
Oncology
Tianye Li, Zhenzhen Hao, Zihan Tang, Chunting Li, Linglin Cheng, Tao Wang, Xiaojin Zhu, Yunhao He, Yongye Huang, Bing Wang
Summary: BAP31 plays an important role in the migration of lung cancer cells, and its knockdown weakens cell migration. Treatment with TGF-beta can reduce cell migration and enhance proliferation, which is downregulated after BAP31 knockdown. Moreover, BAP31 regulates multiple signaling pathways, especially the Wnt signaling pathway.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yuanzhe Zhu, Huala Wu, Xi Yang, Zhijuan Xiong, Tiantian Zhao, Xin Gan
Summary: This study revealed that LINC00514 is upregulated in NSCLC and plays a role in the proliferation, migration, and invasion of NSCLC cells through activation of the Wnt/β-catenin pathway and EMT. Silencing LINC00514 restored the biological phenotypes of NSCLC cells by inhibiting the Wnt/β-catenin pathway. Additionally, silencing LINC00514 attenuated the growth of xenograft tumors in vivo.
Article
Oncology
Jun Zhang, Xian Zhang, Shasha Yang, Yanqiu Bao, Dongyuan Xu, Lan Liu
Summary: The study demonstrated that higher FOXH1 expression is associated with poor overall survival in lung cancer patients. Knockdown of FOXH1 inhibited lung cancer cell proliferation, migration, invasion, and cycle, highlighting FOXH1's potential as a therapeutic target in certain types of lung cancer.
CANCER CELL INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Jian Hou, Suiqing Huang, Yan Long, Kangni Feng, Liqun Shang, Zhuoming Zhou, Yuan Yue, Xiaolin Huang, Guangxian Chen, Zhongkai Wu
Summary: In this study, we investigated the role of DACT1 and its connection with beta-catenin and connexin 43 in human-derived cardiomyocytes. The findings suggest that DACT1 interacts with beta-catenin and connexin 43 in differentiated cardiomyocytes.
Article
Respiratory System
Xiaoshan Su, Junjie Chen, Xiaoping Lin, Xiaoyang Chen, Zhixing Zhu, Weijing Wu, Hai Lin, Jianming Wang, Xiangjia Ye, Yiming Zeng
Summary: This study identified that FERMT3 is downregulated in COPD patients and is associated with CSE-induced EMT. Overexpression of FERMT3 can inhibit CSE-induced EMT and regulate this process through the Wnt/beta-catenin signaling pathway.
RESPIRATORY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Arpan Parichha, Varun Suresh, Mallika Chatterjee, Aditya Kshirsagar, Lihi Ben-Reuven, Tsviya Olender, M. Mark Taketo, Velena Radosevic, Mihaela Bobic-Rasonja, Sara Trnski, Michael J. Holtzman, Natasa Jovanov-Milosevic, Orly Reiner, Shubha Tole
Summary: The choroid plexus, responsible for secreting cerebrospinal fluid, plays a critical role in brain development and function. Activation of the Wnt signaling pathway is essential for the proper development of the choroid plexus, but over-activation of this pathway can lead to a loss of its properties and transformation into neurons.
NATURE COMMUNICATIONS
(2022)
Article
Anatomy & Morphology
Yonghong Lian, Cuiqiong Li, Jianchun Li, Yongxiang Xie, Qiancheng Liu, Minhua Wu, Wei Shi, Lifeng Meng
Summary: This study aimed to investigate the role of connexin 43 (Cx43) in regulating epithelial mesenchymal transformation (EMT) of renal tubular epithelial cells (RTECs) through the Akt/mTOR signaling pathway and the potential inhibitory effect of Astragaloside IV (ASV) on renal interstitial fibrosis by regulating Cx43. The results showed that Cx43 played a significant role in RTECs injury and that ASV could inhibit TGF-beta 1-induced RTECs transdifferentiation. Further investigation of this mechanism may provide promising therapeutic strategies for the treatment of renal interstitial fibrosis.
Article
Biochemistry & Molecular Biology
Jinbao Xie, Guoliang Liao, Zhi Feng, Bo Liu, Xu Li, Minglian Qiu
Summary: This study found that the high expression of ERO1L in LUAD patients is associated with poor prognosis, and is significantly correlated with lymph node metastasis, TNM stage and tumor size. ERO1L promotes the proliferation and metastasis of LUAD by activating the Wnt2/β-catenin signaling pathway.
MOLECULAR CARCINOGENESIS
(2022)
Article
Pharmacology & Pharmacy
Zicong Zhou, Shixiu Liang, Zili Zhou, Jieyi Liu, Xiaojing Meng, Fei Zou, Changhui Yu, Shaoxi Cai
Summary: The study found that avasimibe could reduce the production of inflammatory factors, lower the level of immunoglobulin E, and improve the secretion and distribution of airway epithelial cells in house dust mite-induced asthmatic mice. Furthermore, avasimibe alleviated the changes in adherens junction proteins induced by house dust mites through the regulation of beta-catenin activation and cellular localization, thus improving the integrity of the airway epithelial barrier.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Developmental Biology
Sara E. Billings, Nina M. Myers, Lee Quiruz, Alan G. Cheng
Summary: During mammalian cochlear development, epithelial sensory precursors and periotic mesenchymal cells from distinct lineages coordinate through Wnt activation to promote proliferation and differentiation, driving the transition between epithelial and mesenchymal states in a cell type-dependent manner.
Article
Cell Biology
Qing Song, Zhifen Han, Xinnan Wu, Yan Wang, Lihong Zhou, Liu Yang, Ningning Liu, Hua Sui, Jianfeng Cai, Qing Ji, Qi Li
Summary: In colorectal cancer (CRC), beta-arrestin1 promotes migration and metastasis of CRC cells by regulating the EMT process through the Wnt/β-catenin signaling pathways. This provides a novel therapeutic target for CRC metastasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Huaize Liu, Jie Ding, Karl Koehnlein, Nadine Urban, Alessandro Ori, Pablo Villavicencio-Lorini, Peter Walentek, Lars-Oliver Klotz, Thomas Hollemann, Thorsten Pfirrmann
Article
Biochemistry & Molecular Biology
Karl Koehnlein, Nadine Urban, David Guerrero-Gomez, Holger Steinbrenner, Pavel Urbanek, Josephine Priebs, Philipp Koch, Christoph Kaether, Antonio Miranda-Vizuete, Lars-Oliver Klotz
Article
Geriatrics & Gerontology
Holger Steinbrenner, Lars-Oliver Klotz
ZEITSCHRIFT FUR GERONTOLOGIE UND GERIATRIE
(2020)
Editorial Material
Geriatrics & Gerontology
Lars-Oliver Klotz, Andreas Simm
ZEITSCHRIFT FUR GERONTOLOGIE UND GERIATRIE
(2020)
Article
Biochemistry & Molecular Biology
Sarah Tauber, Maria Katharina Sieckmann, Katrin Erler, Wilhelm Stahl, Lars-Oliver Klotz, Holger Steinbrenner
Summary: The study found that the Se status had no major effect on Nrf2 signaling in HepG2 cells, although electrophiles could enhance the expression of Nrf2 target genes independent of cellular Se status.
Meeting Abstract
Biochemistry & Molecular Biology
Thilo Magnus Philipp, Andreas Will, Karl Koehnlein, Holger Steinbrenner, Lars-Oliver Klotz
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Niklas Krafczyk, Lars-Oliver Klotz
Summary: FOXO family proteins are crucial transcriptional regulators that modulate cellular metabolism and antioxidant defense strategies. They regulate the expression of antioxidant genes to contribute to cellular response to oxidative stress.
Article
Biochemistry & Molecular Biology
Thilo Magnus Philipp, Andreas Will, Hannes Richter, Patrick Rainer Winterhalter, Georg Pohnert, Holger Steinbrenner, Lars-Oliver Klotz
Summary: Methanethiol is a gas produced by microbial degradation of methionine, primarily originating from bacteria in the human large intestine. SELENBP1 has been identified as a methanethiol oxidase, converting it to other substances. Using a novel assay, researchers confirmed the loss of MTO function in SELENBP1 variants and found that mature enterocytes are capable of eliminating microbiome-derived methanethiol.
Article
Biochemistry & Molecular Biology
Thilo Magnus Philipp, Weiye Gong, Karl Koehnlein, Verena Alexia Ohse, Frederike Iris Mueller, Josephine Priebs, Holger Steinbrenner, Lars-Oliver Klotz
Summary: A study identifies SEMO-1 as an enzyme in C. elegans that can degrade methanethiol and confer resistance to selenite. SEMO-1-deficient worms show increased resistance to oxidative stress and extended lifespan.
Article
Biochemistry & Molecular Biology
Anne Sophie Scheller, Thilo Magnus Philipp, Lars-Oliver Klotz, Holger Steinbrenner
Summary: SELENBP1 and CBS are reciprocally regulated during the spontaneous differentiation of human colorectal adenocarcinoma cells, paralleling their opposing regulation in colorectal cancer. Butyrate exposure imitates some aspects of spontaneous differentiation but does not elicit the same expression patterns of genes encoding H2S-modulating enzymes.
Article
Biochemistry & Molecular Biology
Thilo Magnus Philipp, Leon Gernoth, Andreas Will, Maria Schwarz, Verena Alexia Ohse, Anna Patricia Kipp, Holger Steinbrenner, Lars-Oliver Klotz
Summary: Selenium-binding protein 1 (SELENBP1) acts as a methanethiol oxidase (MTO) in humans, converting methanethiol to hydrogen peroxide, hydrogen sulfide, and formaldehyde. Copper ions are essential for this MTO activity, as demonstrated by mutagenesis studies. Selenium binding, on the other hand, is not required for MTO activity. SELENBP1 ortholog SEMO-1 in the nematode C. elegans also requires copper ions for MTO activity. SELENBP1 degrades volatile sulfur compounds (VSCs), including methanethiol, suggesting its role in mitigating malodors.
Review
Biochemistry & Molecular Biology
Thilo Magnus Philipp, Anne Sophie Scheller, Niklas Krafczyk, Lars-Oliver Klotz, Holger Steinbrenner
Summary: Tumor cells develop various metabolic adaptations to cope with increased demands and enhance stress resilience, including dysregulation of sulfur metabolism leading to elevated levels of volatile sulfur compounds (VSCs) in cancer patients. Methanethiol, the predominant cancer-associated VSC, has been proposed as a promising biomarker for non-invasive cancer diagnosis. Gut bacteria, such as Fusobacterium nucleatum, are the major source of methanethiol exposure, and proteins like SELENBP1 and METTL7B are involved in the production and degradation of methanethiol.
Meeting Abstract
Biochemistry & Molecular Biology
Holger Steinbrenner, Mustafa Micoogullari, Ngoc Anh Hoang, Ina Bergheim, Lars-Oliver Klotz, Helmut Sies
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Sarah Tauber, Katharina Sieckmann, Lars-Oliver Klotz, Holger Steinbrenner
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Lars-Oliver Klotz
FREE RADICAL BIOLOGY AND MEDICINE
(2019)