Article
Cell Biology
Hailong Wang, Aiko Inoue, Yanna Lei, Hongxian Wu, Lan Hong, Xian Wu Cheng
Summary: Cathepsins can be found in various parts of the cell, and their functions go beyond just breaking down intracellular protein. They play significant roles in regulating cell functions and immune responses, and are associated with various illnesses. Recent breakthroughs in detection and therapy of cathepsins provide promising prospects for treating related dysfunctions.
CELLULAR SIGNALLING
(2023)
Article
Food Science & Technology
Edi Piasentier, Nicoletta Pizzutti, Giovanna Lippe
Summary: The study measured Cathepsin B activity in hams from the main Italian prosciutto PDOs: Parma, San Daniele, and Toscano. Different PDOs showed significantly different Cathepsin B ripening trends during the curing process, particularly in the initial and mid-curing stages, indicating that processing conditions can influence the quality of prosciutto.
Article
Biochemistry & Molecular Biology
Natasha Buwa, Nivedhika Kannan, Shaunak Kanade, Nagaraj Balasubramanian
Summary: Cell-matrix adhesion regulates Cav1 phosphorylation through FAK at focal adhesions, with FAK more prominently modulating pY14Cav1 levels at higher stiffness.
Article
Chemistry, Analytical
Lei Zhou, Wenmin Hou, Ying Wang, Xia Lin, Jianguo Hu, Jinlong Li, Chen Liu, Hongkai Liu, Hao Li
Summary: To combat COVID-19, researchers propose targeting host proteins instead of the rapidly mutating virus itself. The cathepsin family of proteins, which digest the extracellular matrix to aid virus spreading, shows promise as host targets. A biosensing interface mimicking the extracellular matrix was used to detect cathepsin levels and found that patients with underlying diseases tend to have higher cathepsin activity, confirming its potential as a host target for fighting COVID-19.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Alessandro Di Spiezio, Andre R. A. Marques, Lina Schmidt, Niklas Thiessen, Lisa Gallwitz, Jens Fogh, Udo Bartsch, Paul Saftig
Summary: Neuronal Ceroid Lipofuscinosis (NCL) is a childhood neurodegenerative disorder characterized by disturbed autophagic flow and pathological accumulation of proteins. Therapeutic clearance of protein aggregates was demonstrated in NCL10 mice by dosing with recombinant human pro-cathepsin-D.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Keval Bollavaram, Tiffanie H. Leeman, Maggie W. Lee, Akhil Kulkarni, Sophia G. Upshaw, Jiabei Yang, Hannah Song, Manu O. Platt
Summary: This study identifies the crucial role of proteases, particularly cathepsins B, K, L, S, and V, in SARS-CoV-2 infection and proposes methodologies to identify other proteases involved in viral entry. It emphasizes the importance of considering additional proteases in COVID-19 research, especially in cases of exacerbated damage in inflammatory conditions where these proteases are upregulated.
Article
Biochemistry & Molecular Biology
Atessa Pakfetrat, Zahra Delavarian, Nooshin Mohtasham, Farnaz Mohajer Tehran, Negin Samiee
Summary: This study examined the gene expressions of Cat-B and Cav-1 in oral squamous cell carcinoma (OSCC) and oral lichen planus (OLP), and found that their expressions were significantly higher in OSCC and OLP samples compared to control. The expressions were also associated with the stage and grade of OSCC samples.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Oncology
Jiangping Wang, Minying Zheng, Xiaohui Yang, Xinyue Zhou, Shiwu Zhang
Summary: Cathepsin B (CTSB) is a lysosomal cysteine protease that plays a crucial role in human physiology and pathology. Its expression level and activity are closely associated with disease progression and severity in various human diseases. CTSB is involved in lysosome-related processes and can impact the development of pathological processes through degradation and remodeling of the extracellular matrix. In tumor development, CTSB has both pro-apoptotic and proteolytic enzymatic effects that can reduce or promote malignancy. Targeting CTSB activity or expression may be essential in the treatment of tumor and non-tumor diseases.
Article
Biochemical Research Methods
Gianmarco Concilia, Austin Lai, Peter Thurgood, Elena Pirogova, Sara Baratchi, Khashayar Khoshmanesh
Summary: The study utilizes a unique dynamic gravity pump to generate transient flows in microfluidic systems for investigating mechanotransduction of shear stress in sensitive cells. Customized flow patterns can be generated by adjusting the attributes of the cam. The pump features controllable, simple, low-cost, compact, and modular design, making it suitable for studying shear sensitive cells under transient flows.
Article
Biochemistry & Molecular Biology
Mohammed F. Gholam, Niharika Bala, Yunus E. Dogan, Abdel A. Alli
Summary: The expression of the MARCKS family of proteins in the kidneys is important for regulating the renal epithelial sodium channel (ENaC) and blood pressure. MARCKS function is controlled by post-translational modifications such as myristoylation, phosphorylation, and proteolysis. Proteases that cleave ENaC and MARCKS have been implicated in hypertension. This study investigated MARCKS protein expression and proteolysis, PKC isoform expression, and protease expression and activity in diabetic db/db mouse kidneys. The results suggest that increased proteolysis of renal MARCKS, upregulation of the cathepsin proteolytic pathway, and increased PKC expression may contribute to the development of high blood pressure in diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Timothy J. Mead, Daniel R. Martin, Lauren W. Wang, Stuart A. Cain, Cagri Gulec, Elisabeth Cahill, Joseph Mauch, Dieter Reinhardt, Cecilia Lo, Clair Baldock, Suneel S. Apte, Ernestina Schipani
Summary: This study reveals a proteostatic mechanism contributing to the reduction of fibrillin-2 and dominance of fibrillin-1 during postnatal development. The lack of ADAMTS6 and ADAMTS10 leads to excess fibrillin-2 and impairs skeletal development and signaling. The protease-substrate relationship between ADAMTS6 and fibrillin-2 is established.
Article
Immunology
Mahendra Pal Singh, Chanchal Sharma, Sun Chul Kang
Summary: Research showed that immunomodulation of cathepsin D (CATD) using morin hydrate (MH) can attenuate kidney fibrosis in chronic kidney disease (CKD), improve kidney function, and enhance survival rates by nearly 150% compared to untreated mice.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maura Ferrer-Ferrer, Shaobo Jia, Rahul Kaushik, Jenny Schneeberg, Izabela Figiel, Stepan Aleshin, Andrey Mironov, Motahareh Safari, Renato Frischknecht, Jakub Wlodarczyk, Oleg Senkov, Alexander Dityatev
Summary: This study investigates the functional importance of neurotrypsin (NT) in synaptic plasticity, learning, and memory extinction. The results show that NT-deficient mice exhibit impaired long-term potentiation, contextual fear memory deficits, and sociability deficits. This highlights the crucial role of NT in synaptic formation and strengthening.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Preston Carey, Ethan Low, Elizabeth Harper, M. Sharon Stack
Summary: Proteases, particularly those of the matrix metalloproteinase family, play a crucial role in ovarian cancer progression and metastasis by enhancing migratory ability, ECM breakdown, and tumor support system development. Upregulation of certain proteases influences angiogenesis for tumor growth and development of metastatic implants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elee Shimshoni, Idan Adir, Ran Afik, Inna Solomonov, Anjana Shenoy, Miri Adler, Luca Puricelli, Fabio Sabino, Simonas Savickas, Odelia Mouhadeb, Nathan Gluck, Sigal Fishman, Lael Werner, Tomer-Meir Salame, Dror S. Shouval, Chen Varol, Ulrich auf dem Keller, Alessandro Podesta, Tamar Geiger, Paolo Milani, Uri Alon, Irit Sagi
Summary: This study investigates the extracellular-matrix dynamics during the onset of inflammatory bowel diseases, revealing a unique matrix signature in the colon even before the appearance of clinical symptoms. The presence of a pre-symptomatic matrix mediated by immune cells carrying remodeling enzymes was found, suggesting its relevance to a wide range of diseases, both chronic and acute.