Article
Immunology
Qiuting Yan, Shengshi Huang, Wouter van der Heijden, Marisa Ninivaggi, Lisa van de Wijer, Romy de Laat-Kremers, Andre J. van der Ven, Bas de Laat, Quirijn de Mast
Summary: This study further explores the relationship between abacavir (ABC) use and thrombin generation through thrombin dynamics analysis, and finds that the association between ABC use and thrombin generation may be due to an increase in the prothrombin conversion pathway. In a cross-sectional study of 208 virally suppressed PLHIV, it is found that the total amount and maximum rate of prothrombin conversion are significantly increased in PLHIV on an ABC-containing regimen, indicating that ABC affects the kinetics of prothrombin conversion rather than procoagulant factor levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Efrat Gerstman, Adi Hendler-Neumark, Verena Wulf, Gili Bisker
Summary: Blood coagulation is a crucial defense mechanism involving various clotting factors. This study demonstrates the use of near-infrared fluorescent single-walled carbon nanotubes to visualize and monitor fibrin clotting in real time. The platform offers improved signal-to-noise ratio and allows for the monitoring of coagulation cascade dynamics. It has potential applications in the diagnostics of coagulation disorders and real-time monitoring of the coagulation process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Lisha Yu, Bin Yu, Hao Chen, Xiaoqiang Shang, Min He, Mengchi Lin, Dan Li, Wenzhao Zhang, Zhengzhong Kang, Jiachen Li, Fangjun Wang, Liping Xiao, Qi Wang, Jie Fan
Summary: The study shows that the artificial catalyst trypsin/CaY can perform surface-confined thrombin generation, providing a shortcut for natural blood coagulation cascade. It has been found to be highly effective in achieving rapid hemostasis in massive bleeding and hemophilia animal models, surpassing current clay- or zeolite-based topical agents.
Article
Chemistry, Applied
Yuting Shi, Wenwen Yu, Xiaoqin Liang, Ju Cheng, Yufei Cao, Mingsheng Liu, Yu Fang, Zheng Yang, Hongbin Liu, Hua Wei, Guanghui Zhao
Summary: Preventing arterial hemorrhage is crucial, but current hemostatic materials are not effective in extreme conditions. Therefore, we developed an expansion sponge based on chitosan and plasma, which provides a simpler and more efficient method for treating high-pressure arterial bleeding wounds.
CARBOHYDRATE POLYMERS
(2023)
Review
Critical Care Medicine
Jessica C. Cardenas
Summary: Thrombin plays a central role in coagulation, essential for normal hemostasis. The generation of thrombin in severely injured patients is closely related to the risks of bleeding and thrombotic complications, and understanding the determinants of its production is clinically important.
Article
Allergy
Ping -Ping Cao, Bao-Feng Wang, James E. Norton, Lydia A. Suh, Roderick G. Carter, Whitney W. Stevens, Anna G. Staudacher, Julia H. Huang, Kathryn E. Hulse, Anju T. Peters, Leslie C. Grammer, David B. Conley, Kevin C. Welch, Robert C. Kern, Zheng Liu, Jingying Ye, Robert P. Schleimer
Summary: The study found increased activation of the coagulation cascade in NP tissue compared to control and uncinate tissue from CRS patients, with local production of TF and FVII in sinonasal mucosa, suggesting activation of the extrinsic coagulation pathway in CRSwNP.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2022)
Article
Cardiac & Cardiovascular Systems
Laura C. Burzynski, Alejandra Morales-Maldonado, Amanda Rodgers, Lauren A. Kitt, Melanie Humphry, Nichola Figg, Martin R. Bennett, Murray C. H. Clarke
Summary: Atherosclerosis is driven by multiple processes involving the immune and coagulation systems. Thrombin can activate interleukin-1α, which contributes to atherogenesis. Inhibition of thrombin can reduce atherosclerotic plaque formation, but also results in a more unstable plaque phenotype.
CARDIOVASCULAR RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Jiantong Dong, Itamar Willner
Summary: Native G-quadruplex-regulated temporal biocatalytic circuits, gene polymerization, and transcription processes are emulated by biomimetic, synthetically engineered transcription machineries coupled to reconfigurable G-quadruplex nanostructures. These systems introduce potential therapeutic applications and advance the field of dynamically modulated G-quadruplex DNA nanostructures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Agriculture, Dairy & Animal Science
Barbara Bruno, Cristiana Maurella, Paola Gianella, Giulio Mengozzi, Erica Ferraris, Antonio Borrelli
Summary: This study observed the haemostasis characteristics in dogs undergoing surgery for malignancies, using rotational thromboelastometry and thrombin generation. The results showed a trend of hypercoagulability 24 hours after surgery and a return to pre-surgery state after 2 weeks.
Article
Immunology
Valery Golderman, Marina Ben-Shimon, Nicola Maggio, Amir Dori, Shany Guly Gofrit, Shani Berkowitz, Lamis Qassim, Avital Artan-Furman, Talya Zeimer, Joab Chapman, Efrat Shavit-Stein
Summary: This study synthesized a group of novel molecules named FEAMs, which effectively prevented the molecular and behavioral negative effects of neuroinflammation by modulating the FVII/aPC-EPCR pathway.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Sara Reda, Jens Muller, Anna Pavlova, Behnaz Pezeshkpoor, Johannes Oldenburg, Bernd Potzsch, Heiko Ruhl
Summary: The study investigated the impact of AT mutations on thrombin inhibition kinetics, finding that patients with AT mutations had significantly prolonged plasma half-life of thrombin. Interestingly, compared with controls, patients with AT mutations had higher AT levels in plasma despite reaching a comparable thrombin half-life.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Ruiqi Yin, Vishal Patel, Rida A. Malik, James C. Fredenburgh, Jeffrey I. Weitz
Summary: Polyphosphate (polyP) serves as a template to promote feedback activation of factor XI by thrombin. This study determines the contribution of thrombin exosites 1 and 2 to polyP-induced potentiation of FXI activation. The results show that polyP accelerates FXI activation by thrombin and exosite 2 ligands attenuate this activation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Engineering, Biomedical
Xiaohong Li, Xiongfa Ji, Kun Chen, Muhammad Wajid Ullah, Basen Li, Jiameng Cao, Lin Xiao, Jun Xiao, Guang Yang
Summary: This study successfully developed radiopaque and hemostatic microspheres by synthesizing barium sulfate nanoparticles with local procoagulant activity. The microspheres demonstrated significant hemostatic capacity and superior embolization effects in both in vitro and in vivo experiments.
BIOACTIVE MATERIALS
(2021)
Article
Gastroenterology & Hepatology
Elena Campello, Alberto Zanetto, Cristiana Bulato, Sara Maggiolo, Luca Spiezia, Francesco Paolo Russo, Sabrina Gavasso, Pierluigi Mazzeo, Daniela Tormene, Patrizia Burra, Paolo Angeli, Marco Senzolo, Paolo Simioni
Summary: This study investigated coagulation alterations in ACLF patients compared to AD patients, finding that ACLF patients had lower platelet count, longer INR and activated partial thrombin time. ROTEM results indicated a more pronounced hypocoagulable state in ACLF patients. No correlation was found between these alterations and bleeding complications.
LIVER INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Kazue Takahashi, Nirmal K. Banda, V. Michael Holers, Elizabeth M. Van Cott
Summary: Serine proteases play crucial roles in innate immunity, with involvement in both the coagulation and complement pathways. Recent studies have revealed thrombin-like activity in complement component factor B and its close relationship with Limulus clotting factor C, providing new insights for disease research.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Editorial Material
Medical Laboratory Technology
Eleftherios P. Diamandis, Karl J. Lackner, Mario Plebani
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Editorial Material
Medicine, General & Internal
Panos Macheras, Caitlin Di Paolo, Yanna Pusica, Eleftherios P. Diamandis
Letter
Medical Laboratory Technology
Oscar D. Pons-Belda, Amaia Fernandez-Uriarte, Annie Ren, Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Editorial Material
Oncology
Amaia Fernandez-Uriarte, Oscar D. Pons-Belda, Eleftherios P. Diamandis
Summary: Cancer screening has been an important field of research. Traditional biomarkers are not suitable for population screening. Circulating tumor DNA has emerged as a promising new marker for cancer, but there are still challenges and the accuracy of new screening tests needs further definition.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2022)
Article
Biochemical Research Methods
Stella K. Vasiliou, Panagiota S. Filippou, Sergi Clotet-Freixas, Antoninus Soosaipillai, Ihor Batruch, Foivos Viktor Tsianos, Ana Konvalinka, Eleftherios P. Diamandis
Summary: This study found that AR regulates the metabolism of BT-474 cells by affecting the expression of a large number of genes and proteins. Further pathway analysis suggests that the androgen receptor acts as a tumor suppressor in BT-474 cells.
CLINICAL PROTEOMICS
(2022)
Letter
Medicine, General & Internal
Eleftherios P. Diamandis, Sok-Ja Janket, Harry A. Conte
Letter
Medical Laboratory Technology
Eleftherios. P. P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Ivan Pasic, Annie H. Ren, Ram Vasudevan Nampoothiri, Ioannis Prassas, Jeffrey H. Lipton, Jonas Mattsson, Eleftherios P. Diamandis, Fotios V. Michelis
Summary: Using a novel multiplex antibody-based proximity extension assay, researchers identified serum proteins, including SLAMF7, IL-1ra, BTN3A2, and DAB2, that may potentially serve as biomarkers for acute graft-vs.-host disease.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Annie H. Ren, Panagiota S. Filippou, Antoninus Soosaipillai, Lampros Dimitrakopoulos, Dimitrios Korbakis, Felix Leung, Vathany Kulasingam, Marcus Q. Bernardini, Eleftherios P. Diamandis
Summary: This study explored the potential of using MUC13 as an ovarian cancer biomarker to complement CA125. The results showed that MUC13 could be used to detect non-serous ovarian carcinoma and early-stage disease, in addition to CA125.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Hannu Koistinen, Ruusu-Maaria Kovanen, Morley D. Hollenberg, Antoine Dufour, Evette S. Radisky, Ulf-Hakan Stenman, Jyotsna Batra, Judith Clements, John D. Hooper, Eleftherios Diamandis, Oliver Schilling, Antti Rannikko, Tuomas Mirtti
Summary: Since the proposition of the pro-invasive activity of proteolytic enzymes over 70 years ago, several roles for proteases in cancer progression have been established. About half of the 473 active human proteases are expressed in the prostate and many of the most well-characterized members of this enzyme family are regulated by androgens, hormones essential for development of prostate cancer. Most notably, several kallikrein-related peptidases, including KLK3 (prostate-specific antigen, PSA), the most well-known prostate cancer marker, and type II transmembrane serine proteases, such as TMPRSS2 and matriptase, have been extensively studied and found to promote prostate cancer progression. Recent findings also suggest a critical role for proteases in the development of advanced and aggressive castration-resistant prostate cancer (CRPC). Perhaps the most intriguing evidence for this role comes from studies showing that the protease-activated transmembrane proteins, Notch and CDCP1, are associated with the development of CRPC.
Letter
Medical Laboratory Technology
Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Oncology
Timo-Pekka K. Lehto, Ruusu-Maaria Kovanen, Susanna Lintula, Adrian Malen, Carolin Sturenberg, Andrew Erickson, Olli-Pekka Pulkka, Ulf-Hakan Stenman, Eleftherios P. Diamandis, Antti Rannikko, Tuomas Mirtti, Hannu Koistinen
Summary: This study aimed to investigate the mRNA levels and prognostic impact of all 15 human kallikrein-related peptidases (KLKs) and their targets, proteinase-activated receptors (PARs), in surgically treated prostate cancer (PCa). The results showed that the expression of KLK2, KLK3, KLK4, and KLK15 was closely associated with tumor aggressiveness and prognosis, suggesting their potential as prognostic biomarkers for PCa.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Letter
Medical Laboratory Technology
Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Letter
Medicine, General & Internal
Sok-Ja Janket, Jukka H. Meurman, Eleftherios P. Diamandis
Review
Medicine, General & Internal
Arsani Yousef, Lucianna Ghobrial, Eleftherios P. Diamandis
Summary: Tumors vary in location, tissue type, and histological subtype. This review highlights current classification schemes for tumor heterogeneity and discusses its clinical significance. New molecular techniques offer affordable assessment of tumor heterogeneity, potentially revolutionizing cancer diagnosis and treatment. Standardized and reproducible assessment methods are needed to fully realize the benefits of tumor heterogeneity.