Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 33, Issue 2, Pages 249-254Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.33.249
Keywords
cytochrome P450 3A; fetal membrane; amnion; chorion; decidua; testosterone 6 beta-hydroxylation
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18590138]
- Grants-in-Aid for Scientific Research [18590138] Funding Source: KAKEN
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Expression levels of cytochrome P450 (CYP) 3A4, CYP3A5 and CYP3A7 mRNAs in placentas and fetal membranes, which were split into amnion and chorion leave attached decidua (chorion/decidua), obtained from pregnant women with normal delivery (5 subjects) and Caesarean section (15 subjects) were determined. These CYP3A mRNAs were also expressed in amnion and chorion/decidua together with placenta, although the expression level of these mRNAs was strikingly different between subjects. The expression level of the CYP3A4 mRNA in the placenta was about 2-fold higher than those in amnion and chorion/decidua. On the other hand, the expression levels of CYP3A5 and CYP3A7 mRNAs were highest in chorion/decidua. The immunologically related protein(s) with CYP3A7 was detected in all tissues examined. Testosterone 6 beta-hydroxylase activity in homogenate of human placenta, amnion and chorion/decidua were 26.6, 3.7 and 4.6 pmol/h/mg protein, respectively. These results suggest that CVP3As in fetal membranes have the metabolic function to protect the fetus from exposure to drugs.
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