Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 33, Issue 10, Pages 1673-1679Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.33.1673
Keywords
plant-derived lactic acid bacteria; Enterococcus avium; gamma-aminobutyric acid
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Funding
- Hiroshima Biocluster (a Cooperative Link of Unique Science and Technology for Economy Revitalization), Japan
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In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7 +/- 1-6.4 g/l GABA at a conversion rate of 86.0 +/- 5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
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