Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 31, Issue 8, Pages 1631-1633Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.31.1631
Keywords
recombinant human growth hormone; micropile; percutaneous administration; absorption; rat
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Funding
- MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan
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The feasibility of self-dissolving micropiles (SDMP) as a percutaneous delivery system of recombinant human growth hormone (rhGH) has been studied in rats using SDMP where dextran was used as a base. After mixing dextran solution with rhGH, SDMPs were prepared by pulling with polypropyrene tips. The mean weight, length and diameter were 0.68 +/- 0.05 mg, 3.2 +/- 0.5 mm and 0.6 +/- 0.2 mu m, respectively. To evaluate the bioavailability (BA) of rhGH percutaneously administered by SDMP, an absorption experiment was performed in rats. RhGH SDMPs were inserted into the rats skin, 200 mu g kg(-1), and plasma rhGH levels were measured by an ELISA method. Peak plasma rhGH level, 132.8 +/- 11.8 ng ml(-1), appeared at 0.8 +/- 0.2 h. By comparing the plasma rhGH levels vs. time profiles after the administration of SDMP and intravenous injection of rhGH solution, 5 mu g kg(-1), BA of rhGH from SDMP was calculated to be 87.5%. Theses results may suggest that SDMP can be used as a novel percutaneous drug delivery system.
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