4.3 Article

Somatic embryogenesis and regeneration of Vigna radiata

Journal

BIOLOGIA PLANTARUM
Volume 54, Issue 2, Pages 245-251

Publisher

SPRINGER
DOI: 10.1007/s10535-010-0043-x

Keywords

cell suspension cultures; mung bean; plant growth regulators; primary leaf explant

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An efficient regeneration protocol via somatic embryogenesis was optimized for mung bean [Vigna radiata (L.) Wilczek; cv. Vamban 1]. Primary leaf explants were used for embryogenic callus induction in MMS medium (Murashige and Skoog salts with B5 vitamins) containing 2.0 mg dm(-3) 2,4-dichlorophenoxyacetic acid (2,4-D), 150 mg dm(-3) glutamine and 3 % sucrose. Fast growing, highly embryogenic cell suspensions were established from 21-d-old calli in MMS medium supplemented with 0.5 mg dm(-3) 2,4-D and 50 mg dm(-3) proline (Pro), and maximum recovery of globular (39.0 %), heart-shaped (26.3 %) and torpedo-stage (21.0 %) somatic embryos were observed in this medium. Mature cotyledonary-stage somatic embryos were cultured for 5 d in half strength B5 liquid medium containing 0.05 mg dm(-3) 2,4-D, 20 mg dm(-3) Pro, 5 mu M abscisic acid, 1000 mg dm(-3) KNO(3), 50 mg dm(-3) polyethylene glycol (PEG 6000) and 30 g dm(-3) D-mannitol. Mature somatic embryos were germinated after dessication for 3 d and complete development of plantlets accomplished in MMS medium containing 30 g dm(-3) maltose, 0.5 mg dm(-3) benzyladenine and 500 mg dm(-3) KNO(3). Profuse lateral roots, and regeneration frequency (up to 60 %) were observed in half-strength MMS medium containing 0.5 mg dm(-3) indolebutyric acid (IBA). The regenerated plants were grown to fruiting and were morphologically normal and fertile.

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