Journal
BIOLOGIA PLANTARUM
Volume 54, Issue 1, Pages 141-144Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-010-0022-2
Keywords
Agrobacterium transformation; ipt gene; Solanum tuberosum; transgenic potato
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The transformation of potato (Solanum tuberosum L. cv. Desiree) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm(-3).
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