Journal
BIOLOGIA PLANTARUM
Volume 52, Issue 3, Pages 553-556Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-008-0109-1
Keywords
Triticum aestivum; antibiotics; embryogenic callus; plant tissue culture
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The influences of cefotaxime and carbenicillin on regeneration potential of wheat (Triticum aestivum L.) mature embryos were investigated. Filter-sterilized cefotaxime enhanced regeneration capacity although it did not affect the average number of shoots per explant. The highest regeneration capacity of 55.4 % was obtained on regeneration medium supplemented with 100 mg dm(-3) cefotaxime. Filter-sterilized carbenicillin did not stimulate plant regeneration. However, higher concentration (100 mg dm(-3)) accelerated callus browning and inhibited the following regeneration. Autoclaved antibiotics at all tested concentrations showed detrimental effects on callus morphogenesis and plant regeneration.
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