Journal
BIOLOGIA
Volume 68, Issue 5, Pages 1010-1014Publisher
SPRINGER
DOI: 10.2478/s11756-013-0240-0
Keywords
porcine PID1; 3T3-L1 preadipocyte; proliferation; differentiation
Categories
Funding
- National Natural Science Foundation of China [31201811]
- Sichuan Provincial Education Department [10ZA053]
- Specific Research Supporting Program for Academic Sustentation Research Team in Sichuan Agricultural University
Ask authors/readers for more resources
The phosphotyrosine interaction domain containing 1 (PID1) gene was firstly isolated from obese subjects and involved in obesity-associated insulin resistance. In the present study, DurocxLandracexYorkshire (DLY) pig PID1 cDNA was cloned. The entire open reading frame of the cloned porcine PID1 is 654 bp. The predicted protein is composed of 217 amino acids residues with a molecular mass of 24,774 Da. Over-expression of porcine PID1 significantly accelerated the proliferation of 3T3-L1 preadipocyte, but inhibited preadipocyte differentiation by decreasing the numerous fat droplets appeared and down-regulating the mRNA expression levels of peroxisome proliferators-activated receptor-gamma, CCAAT/enhancer binding protein alpha, fat acid synthase and lipoprotein lipase. Together, these results suggest that porcine PID1 plays a role in regulating adipose development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available