Article
Biochemistry & Molecular Biology
Sung-Ju Lee, Seon-A Jang, Seong Cheol Kim, Jin Ah Ryuk, Hyunil Ha
Summary: The water extract of Lophatherum gracile leaves inhibits osteoclast differentiation and bone loss, making it a potential candidate for therapy and prevention of postmenopausal osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Sung-Ju Lee, Seon-A Jang, Seong Cheol Kim, Dong Ryun Gu, Hyun Yang, Jin Ah Ryuk, Hyunil Ha
Summary: This study found that Euonymus alatus (Thunb.) Siebold has inhibitory effects on osteoclast differentiation and osteoporosis. Through downregulating the expression of RANKL and interfering with RANKL-mediated signaling pathways, it can inhibit the generation and function of osteoclasts. Additionally, oral administration of the ethanolic extract significantly alleviated bone loss in a mouse model. These findings suggest that E. alatus holds potential as a biotherapeutic candidate for anti-postmenopausal osteoporosis.
Article
Microbiology
Yuan-Wei Zhang, Mu-Min Cao, Ying-Juan Li, Ren-Wang Sheng, Ruo-Lan Zhang, Meng-Ting Wu, Jia-Yu Chi, Rui-Xin Zhou, Yun-Feng Rui
Summary: This study aimed to investigate whether supplementation of Prevotella histicola (Ph) could prevent bone loss in mice with ovariectomy (OVX)-mediated osteoporosis (OP), and explore related mechanisms. The results showed that regular and quantitative perfusion of Ph mitigated the bone loss in mice with OVX-mediated OP and improved gut microbiota disturbance.
Article
Plant Sciences
Kalpana Patel, S. V. V. S. Ravi Mangu, Shinde Vijay Sukhdeo, Kunal Sharan
Summary: The study aims to investigate the osteo-protective effect of root and leaf ethanolic extract of Sida cordifolia and its underlying mechanism. The results showed that both RE and LE have antioxidant activity and promote osteoblast differentiation by activating ERK, AKT, and CREB signaling pathways, indirectly inhibiting osteoclast differentiation.
Article
Biochemistry & Molecular Biology
Weiduo Hou, Mo Chen, Chenyi Ye, Erman Chen, Weixu Li, Wei Zhang
Summary: This study found that Parkin protein can inhibit osteoclast formation, reduce bone resorption activity, and increase autophagy activity, showing therapeutic potential for diseases associated with overactive osteoclasts.
Article
Biochemistry & Molecular Biology
Xueqin Gao, Xuying Sun, Haizi Cheng, Joseph J. Ruzbarsky, Michael Mullen, Matthieu Huard, Johnny Huard
Summary: Osteoporosis and age-related bone loss increase fracture risk and impair bone healing. MRL/MpJ mice have been found to have better bone microarchitecture and biomechanical properties compared to wild-type mice. It was discovered that these mice also maintain better bone microarchitecture and higher bone mass during aging and long-term ovariectomy. These findings may be used to develop therapeutic approaches for maintaining bone mass and improving bone regeneration and repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qiang Zhang, Xiaoxia Song, Xiaoming Chen, Ruizhong Jiang, Ke Peng, Xinqiao Tang, Zhong Liu
Summary: The study demonstrated that hesperidin can significantly improve bone density and mineral content in OVX rats by reducing inflammation and oxidative stress. Additionally, hesperidin also enhances the biomechanical parameters of bones.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Endocrinology & Metabolism
Margarita M. Ivanova, Julia Dao, Neil Kasaci, Andrew Friedman, Lauren Noll, Ozlem Goker-Alpan
Summary: This study revealed that elevated levels of sclerostin in GD patients are associated with bone pain, BMI, and EM flask deformity. Most patients with elevated sclerostin also have osteopenia. Additionally, sclerostin levels increase with age in GD patients, and are higher compared to healthy controls in the same age group.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhiyuan Guan, Zheng Xuanqi, Junxiong Zhu, Wanqiong Yuan, Jialin Jia, Chenggui Zhang, Tiantong Sun, Huijie Leng, Changtao Jiang, Yingsheng Xu, Chunli Song
Summary: Postmenopausal osteoporosis is a common bone metabolic disease, and gut microbiota (GM) imbalance plays an important role in its development. This study found that ovariectomized mice had gut microbiota dysbiosis, and reducing gut microbiota through antibiotic treatment improved bone health. G-protein coupled bile acid receptor and systemic inflammatory factors were identified as key players in bone metabolism.
PHARMACOLOGICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Francesca Salamanna, Deyanira Contartese, Francesca Veronesi, Lucia Martini, Milena Fini
Summary: Sheep ovariectomy plays a crucial role in osteoporosis research, and the impact of variables such as breed, age, diet, and time after surgery should be monitored. Comparative studies are necessary to minimize the impact of these variables and to better understand bone remodeling. Results showed a significant decline in bone mineral density and structural parameters after 12 months of ovariectomy, with site-specific bone alterations in the lumbar vertebra, femoral neck, and ribs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Michala Steinerova, Cenek Horecky, Eliska Horecka, Ales Knoll, Sarka Nedomova, Petr Slama, Ales Pavlik
Summary: The Wnt signaling pathway plays a critical role in skeletal development, and targeting this pathway has implications for skeletal health. This study investigated single nucleotide polymorphisms in selected genes involved in this pathway and their associations with bone parameters in hens. Thirteen polymorphisms were found in the GPR177 gene, but only one was associated with bone length, and it was located in an intron. The ESR1 and RUNX2 genes were monomorphic in the tested regions.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Orthopedics
Kewen Zhang, Wuxia Qiu, Hui Li, Jun Li, Pai Wang, Zhihao Chen, Xiao Lin, Airong Qian
Summary: MACF1 plays a positive regulatory role in osteogenesis and bone formation in MSCs through the TCF4/miR-335-5p signaling pathway in SOP, suggesting it as a potential therapeutic target for SOP.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Endocrinology & Metabolism
Yihan Li, Wei-Ju Tseng, Chantal M. J. de Bakker, Hongbo Zhao, Rebecca Chung, X. Sherry Liu
Summary: Postmenopausal osteoporosis is influenced by reduced estrogen levels during menopause, impacting bone remodeling, bone mass, and fracture risk. Peak bone mass and microstructure are important predictors of osteoporosis risk, with thicker trabeculae being associated with lower rates of bone loss in response to estrogen deficiency. Thicker trabeculae are less likely to disconnect or perforate, leading to a lower degree of bone loss.
Article
Cell Biology
Changsheng Yang, Panpan Yang, Peilin Liu, Hong Wang, Ee Ke, Kai Li, Huibo Yan
Summary: This study revealed that FLNA plays a crucial role in osteoporosis by inhibiting osteogenesis and promoting osteoclastogenesis. Inhibiting FLNA and activating the WNT/β-catenin signaling pathway can protect against bone loss in postmenopausal osteoporosis.
CELLULAR SIGNALLING
(2022)
Article
Medicine, Research & Experimental
Ra-Yeong Choi, In-Woo Kim, Moongi Ji, Man-Jeong Paik, Eu-Jin Ban, Joon Ha Lee, Jae Sam Hwang, HaeYong Kweon, Minchul Seo
Summary: The ethanol extract of P. brevitarsis larvae (PBE) can inhibit RANKL-induced osteoclast formation and prevent bone loss. PBE decreases tartrate-resistant acid phosphatase activity and the expression of osteoclastogenesis-associated genes and proteins. It also inhibits the phosphorylation of p38 and NF-κB.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Jessica Gasparello, Chiara Papi, Matteo Zurlo, Laura Gambari, Andrea Rozzi, Alex Manicardi, Roberto Corradini, Roberto Gambari, Alessia Finotti
Summary: Glioblastoma multiforme (GBM) is a highly lethal tumor with no curative treatment available. This study investigated the potential of a combined therapy using sulforaphane (SFN) and a peptide nucleic acid (PNA) to target miR-15b-5p in GBM cells. The results showed that SFN and PNA-a15b synergistically induced apoptosis in GBM cells, suggesting the feasibility of using combined treatments based on PNAs and nutraceuticals to stimulate apoptosis in GBM.
Article
Nutrition & Dietetics
Laura Gambari, Marli Barone, Emanuela Amore, Brunella Grigolo, Giuseppe Filardo, Renato Iori, Valentina Citi, Vincenzo Calderone, Francesco Grassi
Summary: This study found that glucoraphanin, a compound from cruciferous vegetables, can promote osteogenesis in human mesenchymal stromal cells by releasing hydrogen sulfide. This has potential implications for the treatment of osteoporosis.
Article
Engineering, Biomedical
A. Sorriento, A. Cafarelli, P. Spinnato, A. Russo, G. Lisignoli, F. Rabusseau, P. Cabras, E. Dumont, L. Ricotti
Summary: This study aims to provide a standardized method for ultrasound imaging of knee osteoarthritis, making it possible for the technology to be widely used in clinical practice. By developing a wearable brace, untrained subjects were able to acquire ultrasound images comparable to those obtained by expert clinicians. This research is important for enabling tele-ultrasonography.
IEEE JOURNAL OF TRANSLATIONAL ENGINEERING IN HEALTH AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Barbara Roda, Nan Zhang, Laura Gambari, Brunella Grigolo, Cristina Eller-Vainicher, Luigi Gennari, Alessandro Zappi, Stefano Giordani, Valentina Marassi, Andrea Zattoni, Pierluigi Reschiglian, Francesco Grassi
Summary: This study proposes an improved analytical protocol for the MBB RP-HPLC method and validates it. The MBB concentration, temperature, and sample handling were optimized, and the calibration method was validated using leave-one-out cross-validation and tested in a clinical setting. The method shows high sensitivity and allows the quantification of H2S species, with a limit of detection of 0.5 μM. Finally, it can be successfully applied in measurements of H2S levels in the serum of patients subjected to inhalation with vapors rich in H2S.
Article
Biochemistry & Molecular Biology
Diego Trucco, Laura Riacci, Lorenzo Vannozzi, Cristina Manferdini, Lorenzo Arrico, Elena Gabusi, Gina Lisignoli, Leonardo Ricotti
Summary: A stable adhesion to the cartilage is crucial for hydrogels used in cartilage regeneration. Fibrin glue and cellulose nanofibers can improve adhesion strength, while catecholamines do not meet clinically acceptable levels. Stem cells embedded in hydrogels are still suitable for in vivo applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Tommaso Mazzocchi, Daniele Guarnera, Diego Trucco, Francesco Rocco Restaino, Lorenzo Vannozzi, Alessio Siliberto, Gina Lisignoli, Stefano Zaffagnini, Alessandro Russo, Leonardo Ricotti
Summary: Articular cartilage defects and degenerative diseases can cause joint pain and loss of functionality, and current treatments include joint replacement and cartilage regeneration. This study presents an innovative device that allows the direct deposition of multiple biomaterials in an arthroscopic scenario. The device has a flexible tip and multiple coaxial channels, allowing minimally invasive deposition of biomaterials, and experimental tests have demonstrated its safety and effectiveness.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Laura Gambari, Antonella Cellamare, Francesco Grassi, Brunella Grigolo, Alessandro Panciera, Alberto Ruffilli, Cesare Faldini, Giovanna Desando
Summary: Knee osteoarthritis (OA) is a joint disorder characterized by degenerative and inflammatory processes. Bioactive dietary molecules (BDMs), especially polyphenols (PPs), have shown potential in targeting inflammation and pain. However, there are still limitations in terms of biosafety and bioavailability that need to be addressed before their clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Cristina Manferdini, Elena Gabusi, Yasmin Saleh, Enrico Lenzi, Giovanni D'Atri, Leonardo Ricotti, Gina Lisignoli
Summary: This systematic review focuses on the characteristics of hydrogels used for embedding MSCs in in vitro/ex vivo studies, in vivo OA models, and clinical trials for cartilage regeneration in OA. The review identifies that hydrogels have shown promising results in these studies and trials, providing positive impact on patients with OA.
Article
Cardiac & Cardiovascular Systems
Kyu-Won Cho, Mark Andrade, Seongho Bae, Sangsung Kim, Jin Eyun Kim, Er Yearn Jang, Sangho Lee, Ahsan Husain, Roy L. Sutliff, John W. Calvert, Changwon Park, Young-sup Yoon
Summary: The study reveals that CBX7 plays a crucial role in regulating cardiomyocyte proliferation and cardiac regeneration, suggesting that CBX7 could be a potential target for cardiac regeneration.
Review
Endocrinology & Metabolism
Abdul Malik Tyagi
Summary: The gut microbiota plays a crucial role in regulating host metabolism and has been found to have an impact on bone health. This review focuses on the relationship between gut microbiota and bone mass, with a specific emphasis on the probiotic LGG and its mechanisms in regulating bone mass.
ENDOCRINOLOGY DIABETES & METABOLISM
(2023)
Article
Oncology
Jessica Gasparello, Chiara Papi, Matteo Zurlo, Laura Gambari, Alex Manicardi, Andrea Rozzi, Matteo Ferrarini, Roberto Corradini, Roberto Gambari, Alessia Finotti
Summary: Starting from a list of nine dysregulated miRNAs in early-stage CRC patients, the study verified the therapeutic effects of anti-miRNA PNAs targeting the most relevant up-regulated miRNAs. PNAs down-regulated these target miRNAs and induced apoptosis in CRC cellular models. Combining anti-miRNA PNAs with pro-apoptotic compounds like sulforaphane resulted in high levels of apoptosis induction.
Article
Cell Biology
Elisa Schena, Elisabetta Mattioli, Chiara Peres, Laura Zanotti, Paolo Morselli, Patricia Iozzo, Maria Angela Guzzardi, Chiara Bernardini, Monica Forni, Salvatore Nesci, Massimiliano Caprio, Carolina Cecchetti, Uberto Pagotto, Elena Gabusi, Luca Cattini, Gina Lisignoli, William Blalock, Alessandra Gambineri, Giovanna Lattanzi
Summary: This study identifies changes in MR and its interaction with lamin A in FPLD2, and demonstrates that the MR antagonist spironolactone can improve adipocyte differentiation and brown adipose tissue activity in FPLD2 patients.
Article
Chemistry, Applied
Diego Trucco, Lorenzo Vannozzi, Elena Gabusi, Enrico Lenzi, Cristina Manferdini, Alessia Bacci, Liliana Agresti, Maria Rosaria Pascale, Sandra Cristino, Gina Lisignoli, Leonardo Ricotti
Summary: Gellan gum, a polysaccharide similar to glycosaminoglycans, has gained attention in cartilage tissue engineering. In this study, the photo-crosslinkable formulation of gellan gum, called GGMA, was investigated for visible light cross-linking. The sterilization effects, chemical and rheological properties, and cross-linking efficiency of GGMA were evaluated. The findings suggest that GGMA has potential clinical application for treating cartilage defects.
CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Maegan L. Brockman, Lanfang Wang, John W. Calvert, Rebecca D. Levit
CIRCULATION RESEARCH
(2022)
Article
Polymer Science
Alessio Bucciarelli, Mauro Petretta, Brunella Grigolo, Laura Gambari, Alessandra Maria Bossi, Francesco Grassi, Devid Maniglio
Summary: In this study, a crosslinked methacrylated silk (Sil-MA) grid structure was developed, which can recover its shape under UV light and has biocompatibility and osteogenic support. Sil-MA was chemically and physically characterized, and tested for its biocompatibility according to ISO protocols. Digital Light Processing and pneumatic extrusion techniques were used to manufacture the Sil-MA grid structure, demonstrating its shape memory property and potential application in bone regeneration.