Journal
BIOINFORMATICS
Volume 29, Issue 22, Pages 2844-2851Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btt508
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Funding
- National Institutes of Health [NS074686]
- Ellison Medical Foundation
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Motivation: Validation and reproducibility of results is a central and pressing issue in genomics. Several recent embarrassing incidents involving the irreproducibility of high-profile studies have illustrated the importance of this issue and the need for rigorous methods for the assessment of reproducibility. Results: Here, we describe an existing statistical model that is very well suited to this problem. We explain its utility for assessing the reproducibility of validation experiments, and apply it to a genomescale study of adenosine deaminase acting on RNA (ADAR)-mediated RNA editing in Drosophila. We also introduce a statistical method for planning validation experiments that will obtain the tightest reproducibility confidence limits, which, for a fixed total number of experiments, returns the optimal number of replicates for the study.
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