Article
Biotechnology & Applied Microbiology
Masahiro Komeno, Yuki Yoshihara, Junya Kawasaki, Wataru Nabeshima, Koshi Maeda, Yuki Sasaki, Kiyotaka Fujita, Hisashi Ashida
Summary: In this study, the degradation pathways of AX and AXOs by Bifidobacterium longum subsp. longum were characterized, and the crucial roles of BlArafD and BlArafE in the assimilation of AX were identified.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Joseph L. L. Roberts, Mateo Golloshi, Derek B. B. Harding, Madison Conduah, Guanglu Liu, Hicham Drissi
Summary: Age-related delays in bone repair can be improved by dietary supplementation with a beneficial probiotic species, Bifidobacterium longum. This supplementation accelerates bony callus formation, improves mechanical properties of the fractured limb, preserves intestinal barrier, dampens systemic inflammation, and maintains the microbiota community structure. Additionally, B. longum attenuates fracture-induced systemic pathologies. These findings suggest that targeting the gut microbiota using dietary approaches can enhance fracture healing outcomes in the context of aging.
Article
Biochemistry & Molecular Biology
Porntipha Vitheejongjaroen, Anongnard Kasorn, Narathip Puttarat, Fabien Loison, Malai Taweechotipatr
Summary: The results of this study suggest that B. animalis MSMC83 has a beneficial role in alleviating oxidative stress in rats by increasing the expression of antioxidant enzymes and reducing pro-inflammatory cytokines. It also restores microbiota diversity after D-galactose injection.
Article
Biochemistry & Molecular Biology
Shengjie Li, Aoxiang Zhuge, Jiafeng Xia, Shuting Wang, Longxian Lv, Kaicen Wang, Huiyong Jiang, Ren Yan, Liya Yang, Xiaoyuan Bian, Xianwan Jiang, Qiangqiang Wang, Shengyi Han, Lanjuan Li
Summary: This study found that Bifidobacterium longum R0175 can alleviate APAP-induced liver injury by activating the Nrf2 pathway. The gut microbiome plays a crucial role in this process. B. longum R0175 can reduce inflammation, oxidative stress levels, hepatocyte death, and improve microbiome dysbiosis induced by APAP.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Microbiology
Ricardo Romero-Arguelles, Patricia Tamez-Guerra, Guadalupe Gonzalez-Ochoa, Cesar I. Romo-Saenz, Ricardo Gomez-Flores, Lilian Flores-Mendoza, Elizama Aros-Uzarraga
Summary: This study aimed to determine the effect of Bifidobacterium longum and Chlorella sorokiniana on the IFN type I-mediated antiviral response in rotavirus-infected cells. It was found that the combination of B. longum and C. sorokiniana significantly increased the levels of IFN-alpha and IFN-beta in the cells, improving the cellular antiviral immune response.
Article
Nutrition & Dietetics
Shiloh R. Lueschow, Timothy J. Boly, Steven A. Frese, Giorgio Casaburi, Ryan D. Mitchell, Bethany M. Henrick, Steven J. McElroy
Summary: Necrotizing enterocolitis (NEC) is a disease that primarily affects preterm infants and has limited treatment options. Recent research suggests that the probiotic B. infantis EVC001 can prevent NEC by reducing inflammation and promoting epithelial barrier restoration.
Article
Microbiology
Li Zhao, Song Wang, Jiahuan Dong, Jialu Shi, Jiaqi Guan, Deyu Liu, Fei Liu, Bailiang Li, Guicheng Huo
Summary: This study isolated and screened B. longum subsp. longum strains with potential antioxidant properties from the gut microbiota of infants, with strain K5 showing high antioxidant capacity and promoting intestinal epithelial cell proliferation. These findings suggest that B. longum subsp. longum K5 could be a probiotic candidate for infant nutrition.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Cristina Alcantara, Marta Perez, Pol Huedo, Tatiana Altadill, Jordi Espadaler-Mazo, Juan Luis Arques, Manuel Zuniga, Vicente Monedero
Summary: Polyphosphate synthesis in bacteria is involved in physiological processes and plays a role in intestinal homeostasis. Bifidobacteria have a high capacity for polyphosphate production, and genes related to phosphate transport and metabolism are identified in their genomes. Polyphosphate synthesized by Bifidobacterium strains has a strain-dependent effect on epithelial integrity.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Yue Xiao, Chen Wang, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai
Summary: A bioinformatics-based technique was used to identify strain-specific markers for quantifying the population of three distinct B. longum subsp. longum strains in human and mouse fecal samples. Pangenome analysis revealed a high percentage of strain-specific genes in B. longum subsp. longum, and specific genes were identified for each strain. The performance of qPCR primer-based analysis was validated using non-target microorganisms and fecal baseline microbiota, showing successful colonization of the target B. longum strains in the gut after oral administration.
Article
Biochemistry & Molecular Biology
Hongwon Kim, Jaein Shin, Sumin Kim, Siyoung Kim, Byounggook Cho, Sang-jun Park, Gwoncheol Park, Hakdong Shin, Myeong Soo Park, Jongpil Kim
Summary: Studies have shown that alterations in gut microbiota can affect brain function, including cognition and memory, through the gut-brain axis. This study found that administration of Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI can increase the expression of brain-derived neurotrophic factor, leading to protection against age-related brain damage and improvement of cognitive and memory deficits in aged mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Alexandra Wallimann, Maria Hildebrand, David Groeger, Barbara Stanic, Cezmi A. Akdis, Stephan Zeiter, R. Geoff Richards, T. Fintan Moriarty, Liam O'Mahony, Keith Thompson
Summary: EPS624 can prevent the formation of osteoclasts under inflammatory conditions and slightly increase mineralized matrix deposition in osteogenic cell cultures. However, the inhibitory effect of EPS624 on osteoclast formation is weakened or completely prevented in the presence of an anti-TLR2 blocking antibody or MyD88(-/-) osteoclast precursors, respectively. Additionally, EPS624 induces IL-10 production in osteoclast precursors in a TLR2-dependent manner, although IL-10 is not necessary for EPS624-mediated inhibition of osteoclast formation. EPS624-producing B. longum35624 (R) partially prevents bone loss in Ovx mice when administered orally.
CALCIFIED TISSUE INTERNATIONAL
(2021)
Article
Agriculture, Multidisciplinary
Md Shamim Rahman, Inseok Kang, Youri Lee, Md Ahasun Habib, Byeong Jo Choi, Jong Soon Kang, Doo-Sang Park, Yong-Sik Kim
Summary: The study demonstrates the role of Bifidobacterium longum subsp. infantis YB0411 in inhibiting adipogenesis by reducing triglyceride accumulation and related gene expression, lowering levels of autophagic markers, and exerting anti-obesity effects through AMP-activated protein kinase. YB supplementation also significantly reduced body weight and fat accretion in mice with high-fat-diet-induced obesity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Yan Wang, Yafei Liu, Ives Ivusic Polic, Ajila Chandran Matheyambath, Gisele LaPointe
Summary: The study showed that combining a potential prebiotic (arabinogalactan) and a probiotic strain (Bifidobacterium longum) increased short-chain fatty acid (SCFA) production and promoted the growth of specific gut commensals, which could have a beneficial effect on gut health.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Agriculture, Multidisciplinary
Jiaying Wu, Shumao Cui, Xin Tang, Qiuxiang Zhang, Yan Jin, Jianxin Zhao, Bingyong Mao, Hao Zhang
Summary: This study screened probiotics with the ability to metabolize glucoraphanin and investigated the effects of a combination of B. longum CCFM1206 and BSE on colitis induced by DSS in mice. The results showed that the combination treatment could alleviate colitis symptoms, reduce inflammatory reactions and oxidative stress, and protect the intestinal barrier. Furthermore, the combination increased the production of sulforaphane compounds in the colon and activated genes related to the Nrf2 signaling pathway. These findings suggest that probiotics can enhance the bioactivity of dietary phytochemicals in vivo and have potential therapeutic implications for colitis treatment.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Fangfang Cao, Lulu Jin, Yong Gao, Yuan Ding, Hongyang Wen, Zhefeng Qian, Chenyin Zhang, Liangjie Hong, Huang Yang, Jiaojiao Zhang, Zongrui Tong, Weilin Wang, Xiaoyuan Chen, Zhengwei Mao
Summary: Combining artificial enzymes with probiotics, modified probiotics are used to treat inflammatory bowel disease, overcoming limitations of traditional treatments and showing promising outcomes.
NATURE NANOTECHNOLOGY
(2023)
Correction
Biotechnology & Applied Microbiology
T. S. Oberg, J. L. Steele, S. C. Ingham, V. V. Smeianov, E. P. Briczinski, A. Abdalla, J. R. Broadbent
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2018)
Review
Nutrition & Dietetics
Korry J. Hintze, Abby D. Benninghoff, Clara E. Cho, Robert E. Ward
ADVANCES IN NUTRITION
(2018)
Article
Biochemical Research Methods
Dennis L. Welker, Bryan M. Coburn, John H. McClatchy, Jeff R. Broadbent
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Biotechnology & Applied Microbiology
Fernanda C. Firmino, Davide Porcellato, Madison Cox, Garret Suen, Jeffery R. Broadbent, James L. Steele
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Article
Biochemical Research Methods
Dennis L. Welker, Bailey L. Crowley, Justin B. Evans, Martin H. Welker, Jeff R. Broadbent, Robert F. Roberts, David A. Mills
JOURNAL OF MICROBIOLOGICAL METHODS
(2020)
Article
Biochemistry & Molecular Biology
Ye Li, Korry J. Hintze, Robert E. Ward
Summary: This study evaluated the effects of probiotics, prebiotics, and a bioactive protein fraction on the gut microbiome in mice, finding significant impacts on the composition of the microbiome and minor effects on gut short-chain fatty acid levels, with no effect on gut inflammation. The treatments showed changes in beta-diversity and specific bacterial taxa in the gut microbiome.
Article
Microbiology
Minghao Li, Charles E. Carpenter, Jeff R. Broadbent
Summary: This study investigated the impact of habituation to organic acids on the virulence and gene expression of L. monocytogenes strains, revealing that habituation to organic acids increased pathogenicity and stress response gene expression.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Luis J. Bastarrachea, David W. Britt, Robert E. Ward, Ali Demirci
Summary: The modification of polypropylene with chitosan was found to enhance the generation of Lactobacillus casei biofilms and their lactic acid production. Biofilms grown in rich media produced significantly more lactic acid, while the chitosan-modified polypropylene harbored more cells compared to the control. The deposition of nutrients and biomass on the chitosan-modified polypropylene was confirmed to be higher, with thicker biofilms observed when grown in rich media.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Agriculture, Dairy & Animal Science
Ireland R. Green, Craig J. Oberg, Jeffery R. Broadbent, Randall K. Thunell, Donald J. McMahon
Summary: The study demonstrates that the use of galactose-fermenting adjunct cultures can effectively reduce gas production by Paucilactobacillus wasatchensis in Cheddar cheese, preventing issues like cracks in the cheese. This is achieved by co-inoculating lactose-negative galactose-positive adjunct cultures with Pa. wasatchensis, leading to a reduction in galactose availability and subsequently limiting the growth of the gas-producing bacteria.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Savannah R. Branson, Jeff R. Broadbent, Charles E. Carpenter
Summary: Organic acids are commonly used in the food industry to control microbial pathogens such as Listeria monocytogenes and Escherichia coli. Research shows that the intracellular accumulation of acid anions is a major inhibitor to cell viability, and some bacteria are able to combat this through active metabolism at lower intracellular pH levels.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Busra Aktas, Mateo Budinich, Lulu Hoza, Scott A. Rankin, Jeff R. Broadbent, James L. Steele
Summary: This study evaluated the survival ability of L. casei in model yogurt and fluid milk, and its impact on pH, organic acids, and sensory attributes of these products. The results showed strain-specific differences in cell densities and metabolic activities during shelf-life. Careful strain selection is necessary to identify probiotic L. casei strains that can survive and maintain product quality in yogurt or fluid milk.
FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
(2023)
Article
Information Science & Library Science
Jeff R. Broadbent, Andrea Payant, Kevin Peterson, Betty Rozum, Liz Woolcott
JOURNAL OF ACADEMIC LIBRARIANSHIP
(2018)
Meeting Abstract
Biochemistry & Molecular Biology
Korry Hintze, Takumaru Hisatome, Sara Kellen, Ashli Hunter, Nicklas Aardema, Robert Ward, Abby Benninghoff, Michael Lefevre
Meeting Abstract
Biochemistry & Molecular Biology
Abby Benninghoff, Ashli Hunter, Stephany Monsanto, Sumira Phatak, Korry Hintze, Robert Ward, Michael Lefevre
Review
Food Science & Technology
Korry J. Hintze, Abby D. Benninghoff, Robert E. Ward
FUNCTIONAL FOODS IN HEALTH AND DISEASE
(2017)