4.5 Article

Improved genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB 7/3/14 as established by deep mate-pair sequencing on the MiSeq (Illumina) system

Journal

JOURNAL OF BIOTECHNOLOGY
Volume 203, Issue -, Pages 19-21

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2015.03.005

Keywords

Rhizoctonia solani AG1-IB; Plant pathogenic fungus; Ultrafast sequencing; AUGUSTUS

Funding

  1. CLIB Graduate Cluster 'Industrial Biotechnology'
  2. Ministry of Innovation of North Rhine-Westphalia
  3. BMBF [FKZ 0315654B]

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The phytopathogenic fungus Rhizoctonia solani AG1-IB of the phylum Basidiomycota affects various economically important crops comprising bean, rice, soybean, figs, cabbage and lettuce. The R. solani isolate 7/3/14 of the anastomosis group AG1-IB was deeply resequenced on the Illumina MiSeq system applying the mate-pair mode to improve its genome sequence. Assembly of obtained sequence reads significantly reduced the amount of scaffolds and improved the genome sequence of the isolate compared to the previous sequencing approach. The genome sequence of the AG1-IB isolate 7/3/14 now provides an up-graded basis to analyze genome features predicted to play a role in pathogenesis and for the development of strategies to antagonize the pathogenic impact of this fungus. (C) 2015 Elsevier B.V. All rights reserved.

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