Journal
JOURNAL OF BIOTECHNOLOGY
Volume 214, Issue -, Pages 27-32Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2015.09.001
Keywords
Halohydrin dehalogenase; Azide; Cyanide; Gas chromatograph
Categories
Funding
- National Natural Science Foundation of China [21176224]
- 973 Program [2011CB710806]
- Natural Science Foundation of Zhejiang Province of China [Z4080032, R3110155]
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A halohydrin dehalogenase (HHDH-PL) from Parvibaculum lavamentivorans DS-1 was characterized and applied to determine azide and cyanide in the water. In this methodology, HHDH-PL catalysed azide and cyanide to react with butylene oxide and form corresponding beta-substituted alcohols 1-azidobutan-2-ol (ABO) and 3-hydroxypentanenitrile (HPN) that could be quantitatively detected by gas chromatograph. The detection calibration curves for azide (R-2= 0.997) and cyanide (R-2 = 0.995) were linear and the lower limits of detection for azide and cyanide were 0.1 and 0.3 mM, respectively. Several other nucleophiles were identified having no effect on the analysis of azide and cyanide, excepting nitrite which influenced the detection of cyanide. This was the first report of a biological method to determine the inorganic azide and cyanide by converting them to the measurable organics. (C) 2015 Elsevier B.V. All rights reserved.
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