Journal
BIOELECTROCHEMISTRY
Volume 85, Issue -, Pages 1-6Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2011.11.002
Keywords
Adsorption; Alcohol dehydrogenase; Nanodiamonds; Bioelectrochemistry
Funding
- NASA-URC Center for Advanced Nanoscale Materials at the University of Puerto Rico Rio Piedras Campus [NNX08BA48A]
- NASA [NNG05GG78H, NNX08AV42H]
- Puerto Rico Development Company (PRIDCO)
- NASA [94413, NNX08AV42H] Funding Source: Federal RePORTER
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0923021] Funding Source: National Science Foundation
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Diamond nanoparticles are considered a biocompatible material mainly due to their non-cytotoxicity and remarkable cellular uptake. Model proteins such as cytochrome c and lysozyme have been physically adsorbed onto diamond nanoparticles, proving it to be a suitable surface for high protein loading. Herein, we explore the non-covalent immobilization of the redox enzyme alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae (E.C.1.1.1.1) onto oxidized diamond nanoparticles for bioelectrochemical applications. Diamond nanoparticles were first oxidized and physically characterized by X-ray diffraction (XRD), FT-IR and TEM. Langmuir isotherms were constructed to investigate the ADH adsorption onto the diamond nanoparticles as a function of pH. It was found that a higher packing density is achieved at the isoelectric point of the enzyme. Moreover, the relative activity of the immobilized enzyme on diamond nanoparticles was addressed under optimum pH conditions able to retain up to 70% of its initial activity. Thereafter, an ethanol bioelectrochemical cell was constructed by employing the immobilized alcohol dehydrogenase onto diamond nanoparticles, this being able to provide a current increment of 72% when compared to the blank solution. The results of this investigation suggest that this technology may be useful for the construction of alcohol biosensors or biofuel cells in the near future. (C) 2011 Elsevier BM. All rights reserved.
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