Article
Energy & Fuels
Aimi Yoshida, Seiya Tsujimura
Summary: This study investigates the impact of varying anions in an electrolyte solution on current generation by a redox hydrogel electrode. Different anions either increase or decrease the catalytic current generated by the electrode, with chaotropic anions like nitrate and chloride affecting FAD-GDH and GOx electrodes differently. Adsorption of anions onto the enzyme surface can induce a negative charge, altering electrostatic interactions and influencing current generation. Hydrogel shrinkage due to kosmotropic anions like sulphate and phosphate can increase catalytic current by changing enzyme and mediator concentrations within the hydrogel architecture. Understanding ion identity is crucial for designing high-performance redox hydrogel electrodes.
JOURNAL OF PHYSICS-ENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Feronika Heppy Sriherfyna, Minenosuke Matsutani, Kensuke Hirano, Hisashi Koike, Naoya Kataoka, Tetsuo Yamashita, Eiko Nakamaru-Ogiso, Kazunobu Matsushita, Toshiharu Yakushi
Summary: Gluconobacter oxydans oxidizes glucose in the periplasmic space independently of nicotinamide cofactors, but if this oxidation system is eliminated, glucose is oxidized in the cytoplasm. Two types of NADH dehydrogenase in G. oxydans have different substrate specificities, with the auxiliary NDH playing a crucial role in maintaining cell growth in the absence of the periplasmic oxidation system.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Simona Ferraraccio, Paolo Bertoncello
Summary: In this study, dehydrogenase enzymes for glucose and lactate were encapsulated in alginate hydrogels and deposited on glassy carbon electrodes. These enzyme modified alginate hydrogels were used as electrochemiluminescence (ECL)-based biosensors for the detection of glucose and lactate. The ECL response was obtained through the redox reaction between the substrate, NAD+, and the encapsulated enzyme. The results suggest that the encapsulated dehydrogenases have potential for the development of biocompatible sensors for glucose and lactate detection in physiological fluids.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Cuiqing Huang, Yuan Xu, Duo Wang, Zerong Chen, Weimin Fang, Changzheng Shi, Zeyu Xiao, Liangping Luo
Summary: This study successfully enhanced the efficacy of sonodynamic therapy by interfering with the redox homeostasis in breast cancer cells. The results of both in vitro and in vivo experiments demonstrated a new strategy for breast cancer treatment.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ningning Wang, Xuewei Cao, Daxi Sun, Xinyu Li, Geng Tian, Jiankai Feng, Pengfei Wei
Summary: A polymer dots (Pdots)-based electrochemiluminescence (ECL) biosensor was developed for the detection of NADH and three metabolites. The biosensor exhibited wide detection ranges, high selectivity, and low limits of detection for glucose, lactic acid, and pyruvate. This biosensor has the potential to be a powerful tool for point-of-care use and disease monitoring.
Article
Chemistry, Analytical
Kyuseok Lee, Gyeoungsun Song, Jungwook Kwon, Jihyeon Kim, Haesik Yang
Summary: In this study, a zwitterionic phenazine compound, mPPS, was used as an organic catalyst and electron mediator for the oxidation of NADH and the detection of glucose and LDH. mPPS exhibited high stability in phosphate buffered saline and its reduced form displayed good water solubility.
Article
Biotechnology & Applied Microbiology
Tomoya Maeda, Abigail Koch-Koerfges, Michael Bott
Summary: The oxidation of NADH and reduction of quinone is crucial for cellular metabolism. In this study with Corynebacterium glutamicum, Ndh was found to be the main enzyme for NADH oxidation, while Mdh and LdhA can functionally replace Ndh. The study also showed that Mdh and LdhA, along with Mqo and LldD, play active roles in NADH oxidation and menaquinone reduction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Leonardo Tensi, Luca Rocchigiani, Gabriel Menendez Rodriguez, Edoardo Mosconi, Cristiano Zuccaccia, Filippo De Angelis, Alceo Macchioni
Summary: The reaction mechanism of nicotine amide dinucleotide hydrogenation (NAD(+) to NADH) catalyzed by Cp*Ir(pyridine-2-sulfonamidate) complexes in the presence of phosphonic acid has been elucidated. The enhanced performance of these catalysts stems from the hemilability of the pyridine ligand, which is displaced during P-H bond activation and facilitates the generation of the metal-hydride intermediate. Experimental results are supported by DFT calculations showing the importance of hydrogen bonding interactions in the activation of phosphite anions. Direct comparison between the unsubstituted catalyst and the 6-aminopyridine-2-sulfonamidate derivative reveals the molecular origin of the latter's superior performance, paving the way for the intelligent design of better catalysts for NADH regeneration.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Ke Jiang, Li Li, Shuxin Liu, Yudong Xu, Jiayao Yang, Wanying Chu, Ping Lu, Ting Gao, Ruoxuan Bai, Fangxu Xu, Hongxin Zhao
Summary: In this study, mutants of E. aerogenes were constructed using different strategies to enhance hydrogen production by regulating NADH supply. The hydrogen yields of the mutants IAM1183-E, IAM1183-L, and IAM1183-EL increased by 23.3%, 81.7%, and 97.9%, respectively, compared to the wildtype strain. Overexpression of the pncB gene also led to increased hydrogen yields in the mutants. The strain IAM1183-EL/P exhibited the highest production efficiency with a 58% higher total hydrogen yield than the wildtype. Metabolite analysis revealed that the mutants with nuoE and ldhA gene deletions, combined with pncB overexpression, resulted in a redistribution of metabolic fluxes, leading to improved hydrogen yield.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Multidisciplinary Sciences
Margarida Duarte, Cleide Ferreira, Gurleen Kaur Khandpur, Tamara Flohr, Jannik Zimmermann, Helena Castro, Johannes M. Herrmann, Bruce Morgan, Ana M. Tomas
Summary: The study shows that type II NADH dehydrogenase is essential in Leishmania parasites, even in the presence of complex I, while complex I can be dispensable in both species. This suggests unexplored functions for NDH2 and validates it as a candidate target for drugs against Leishmania parasites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Motaher M. Hossain, Jannatul Morshed, Seiya Tsujimura
Summary: A bio-conjugated redox network matrix has been developed on a glassy carbon electrode through covalent bonding with one-pot crosslinking, using glucose dehydrogenase, thionine, and poly(ethylene glycol) diglycidyl ether as components. This matrix allows for a glucose oxidation current of 400 mu A cm(-2) at 25 degrees C, with electrons from the enzyme diffusing through the network.
CHEMICAL COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Bo Zhang, Christopher Lingga, Hannah De Groot, Timothy J. Hackmann
Summary: Propionate, a microbial metabolite formed in the gastrointestinal tract, affects host physiology. This study identifies Rnf as a key oxidoreductase in rumen bacteria, which solves the imbalance of electron carriers and cofactors by oxidizing ferredoxin and reducing NAD.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Won-Yong Jeon, Hyug-Han Kim, Young-Bong Choi
Summary: The study focuses on using PDA-MWCNTs to effectively attach Ru(dmo-bpy)(2)Cl-2 and GDH onto SPCEs for glucose detection without needing a cross-linker. Experimental results show a wide linear range of glucose-concentration-dependent responses and the MPS technique facilitates selective detection of glucose. The ease of device fabrication and high detection performance make this a promising pathway for developing glucose sensors based on the GDH enzyme and Ru(dmo-bpy)(2)Cl-2 redox mediator.
Article
Biochemistry & Molecular Biology
Jean-Claude Farre, Paul Li, Suresh Subramani
Summary: This study investigates the localization of NADH-shuttling proteins in Pichia pastoris and introduces a novel BiFC variant called divergent BiFC for detecting colocalization of noninteracting proteins. The findings demonstrate a partial peroxisomal location of a specific NADH-shuttling protein only under certain growth conditions, shedding light on the medium-dependent compartmentalization of proteins in the yeast.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Carlos Alberto Garcia Mogollon, Juan Carlos Quintero Diaz, Jorge Omar Gil Posada
Summary: This manuscript examines the impact of modifying the extracellular redox potential on the production of acetone, butanol, and ethanol in a microbial fuel cell. The addition of NADH enhances acetone production, while adjusting the cathode potential favors butanol production. The findings highlight the potential of bio-electrochemical systems to improve conventional fermentation processes.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xuedong Huang, Binxiao Li, Yanwei Lu, Yixin Liu, Shurong Wang, Neso Sojic, Dechen Jiang, Baohong Liu
Summary: Nanoconfinement in mesoporous nanoarchitectures can significantly influence molecular transport and reaction kinetics in electrochemical processes. A comprehensive understanding of nanoconfinement and mass transport is crucial for practical applications, but a proper approach to study it is currently lacking. In this work, we present a single nanoreactor electrochemiluminescence (SNECL) microscopy technique that utilizes Ru(bpy)(3)(2+)-loaded mesoporous silica nanoparticles to directly visualize and investigate the nanoconfinement-enhanced electrochemical reactions at the single molecule level. Additionally, we monitor and simulate the mass transport capability of single nanoreactors with high spatial resolution through analysis of decay time and recovery ability. The nanoconfinement effects in our system also enable the imaging of single proteins on cellular membranes. This SNECL approach could serve as a promising tool for unraveling the nanoconfinement effects during electrochemical processes and bridging the gap between mesoporous nanoarchitectures and potential electrochemical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Anne de Poulpiquet, Neso Sojic, Laurent Bouffier, Alexander Kuhn, Dodzi Zigah
Summary: Electrochemistry is an important topic taught in undergraduate chemistry programs, but students often find it difficult due to the combination of various physical concepts involved. Bipolar electrochemistry is of special interest for teaching purposes, as it has a simple setup and allows for the tuning of electrochemical reactions through manipulating the electric field. Basic experiments using a commercially available light emitting diode (LED) as a bipolar electrode are introduced here, illustrating the key parameters driving electrochemical reactions and providing an eye-catching introduction to general electrochemistry.
JOURNAL OF CHEMICAL EDUCATION
(2023)
Article
Chemistry, Analytical
Elena Korina, Anton Abramyan, Oleg Bol'shakov, Vyacheslav V. Avdin, Sladjana Savic, Dragan Manojlovic, Vesna Stankovic, Dalibor M. Stankovic
Summary: Stable, water-soluble titanium complex with mandelic acid was used as a precursor to synthesize titanium phosphorus double oxide through hydrothermal conditions in the presence of phosphoric acid. The hydrolysis of organic complexes unexpectedly resulted in the formation of microstructured spheres with narrow size distribution, low aggregation, and minimal morphological irregularities. These microspheres exhibited a complex structure comprising of flakes, with the size manipulated by temperature conditions. The synthesized microspheres-based electrochemical sensors showed successful application in natural water reservoir analysis, with low levels of detection (0.61 µM), a wide dynamic linear range (2-200 µM), good selectivity, excellent reproducibility, and stability.
Article
Chemistry, Multidisciplinary
Emily Kerr, Sara Knezevic, Paul S. Francis, Conor F. Hogan, Giovanni Valenti, Francesco Paolucci, Frederic Kanoufi, Neso Sojic
Summary: In this study, a novel approach to enhancing conventional heterogeneous electrochemiluminescence (ECL) assays is proposed by incorporating a second metal coordination complex, [Ir(sppy)3]3-. ECL microscopy is used to map the spatial distribution of ECL emission from [Ru(bpy)3]2+ labels on the surface of a bead and solution-phase emission from [Ir(sppy)3]3-. The developed [Ir(sppy)3]3--mediated enhancement approach significantly improves the ECL signal from [Ru(bpy)3]2+ labels immobilized on a polystyrene bead, with a 70.9-fold increase at 0.9 V and a 2.9-fold increase at 1.2 V vs Ag/AgCl. This enhancement has important implications for improving existing ECL assays and ECL-based microscopy.
Article
Chemistry, Analytical
Sara Knezevic, Emily Kerr, Bertrand Goudeau, Giovanni Valenti, Francesco Paolucci, Paul S. Francis, Frederic Kanoufi, Neso Sojic
Summary: Electrochemiluminescence (ECL) microscopy is a new technique used for imaging single entities and cells. In this study, a bimodal and bicolor approach was developed to record both positive ECL (PECL) and shadow label-free ECL (SECL) images of single cells. This dual approach enables the imaging of cell morphology and can contribute to multimodal ECL imaging and bioassays.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Julie Descamps, Camille Colin, Gilles Tessier, Stephane Arbault, Neso Sojic
Summary: Here, a label-free electrochemiluminescence (ECL) microscopy using significantly low concentrations of the [Ru(bpy)(3)](2+) luminophore is reported. This study addresses the minimal concentration requirement of the ECL luminophore for imaging single entities. The authors demonstrate the ability to capture ECL images of cells and mitochondria at concentrations as low as nM and pM, which is seven orders of magnitude lower than conventional concentrations. The obtained ECL images exhibit sharp negative optical contrast and the method is concluded to be a simple, fast, and highly sensitive approach for ultrasensitive ECL imaging and reactivity at the single molecule level.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Electrochemistry
Yiran Zhao, Julie Descamps, Yoan Leger, Lionel Santinacci, Sandrine Zanna, Neso Sojic, Gabriel Loget
Summary: n-Si/SiOx/Ni photoanodes were studied for upconversion photoinduced electrochemiluminescence (PECL) in a 3-aminophthalhydrazide (luminol)-hydrogen peroxide (H2O2) model electrochemiluminescent (ECL) system at pH 13. The photoanode exhibited PECL emission at 440 nm when excited at 850 nm, showing an anti-Stokes shift of -410 nm at biases above 0 V vs Ag/AgCl. The alkaline pH value of the electrolyte prevented Ni dissolution, resulting in a longer active period (several hours) compared to similar photoanodes operating at neutral pH.
ELECTROCHIMICA ACTA
(2023)
Review
Biochemical Research Methods
Mathavan Sornambigai, Laurent Bouffier, Neso Sojic, Shanmugam Senthil Kumar
Summary: In recent years, electrochemiluminescence (ECL) has become a highly successful tool in analytical science, with a focus on solid-state ECL for its enhanced electron transfer kinetics and rapid response time. The key element in ECL is the luminophore, with Ru(bpy)(3)(2+) being the gold-standard in ECL. This review provides insights into the principles of ECL and classical reaction mechanisms, as well as fabrication methods and the importance of solid-state ECL over solution-phase ECL. It also discusses emerging technologies and potential applications of solid-state ECL, along with recent advances in hyphenated techniques.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Dongni Han, Danjun Fang, Giovanni Valenti, Francesco Paolucci, Frederic Kanoufi, Dechen Jiang, Neso Sojic
Summary: Electrochemiluminescence (ECL) is an electrochemical technique that offers an optical readout and has recently evolved into a powerful microscopy technique. This study reveals the reaction kinetics of ECL through dynamic imaging, providing a new method for exploring the dynamics and mechanisms of ECL in bioassays and cell microscopy.
ANALYTICAL CHEMISTRY
(2023)
Article
Electrochemistry
Tommaso Nicolini, Youness Boukarkour, Stephane Reculusa, Neso Sojic, Alexander Kuhn, Gerardo Salinas
Summary: Electrochemical water-splitting is essential for storing energy from sustainable sources. This study introduces a novel method based on bipolar electrochemistry, using a conducting polymer layer as a variable-resistance switch to evaluate the electrocatalytic activity of electrode materials for hydrogen and oxygen evolution. The method was validated for Au, Pt, and Ni surfaces, with the results in agreement with classical electrochemical methods. This work provides an easy and straightforward approach for assessing the electrocatalytic activity of electrode materials in water-splitting.
ELECTROCHIMICA ACTA
(2023)
Article
Electrochemistry
Gerardo Salinas, Laurent Bouffier, Neso Sojic, Alexander Kuhn
Summary: Thermodynamics is an intriguing aspect of physical chemistry that is involved in various natural phenomena. The spontaneity of a chemical reaction is directly related to fundamental thermodynamic parameters such as the Gibbs free energy change. The visualization of electromotive force allows students to understand the underlying concepts of spontaneity in coupled reactions. This study introduces the thermodynamic concept of spontaneity to undergraduate students using the concepts of endogenous and exogenous bipolar electrochemistry. By encoding the chemical information of two coupled reactions through the light emission of a light-emitting diode used as a bipolar electrode, the thermodynamic information of the involved redox reactions can be directly read out.
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yiran Zhao, Julie Descamps, Nour al Hoda Al Bast, Marcos Duque, Jaume Esteve, Borja Sepulveda, Gabriel Loget, Neso Sojic
Summary: A new all-optical strategy for electrochemiluminescence (ECL) is reported, which eliminates the need for an external power supply and electrical wires. The fully integrated all-optical wireless device utilizes a modified nanostructured Si photovoltaic junction to induce ECL emission under illumination. This breakthrough opens up new opportunities for developing portable sensing devices.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Physical
Milica Sentic, Ivana Trajkovic, Dragan Manojlovic, Dalibor Stankovic, Maria Vesna Nikolic, Neso Sojic, Jasmina Vidic
Summary: This review presents the application of metal-organic frameworks (MOFs) in electrochemiluminescent (ECL) sensing for water pollutant detection. MOF composites enable the sensitive monitoring of common emerging contaminants such as heavy metals, volatile organic compounds, pharmaceuticals, industrial chemicals, and cyanotoxins.
Article
Chemistry, Physical
Sabrina Bichon, Priscilla Staigre, Sebastien Gounel, Tatjana Safarik, Cristina Carucci, Alexander Kuhn, Nicolas Mano
Summary: The researchers have developed a strategy to stabilize and maintain enzyme activity in mixed-solvent systems by using engineered enzymes and tuned osmium-based redox polymers. They have validated this approach and demonstrated the wide applicability of the electrode in various organic solvents.
JOURNAL OF CATALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Julie Descamps, Yiran Zhao, Julie Le-Pouliquen, Bertrand Goudeau, Patrick Garrigue, Karine Tavernier, Yoan Leger, Gabriel Loget, Neso Sojic
Summary: Localized photoinduced electrochemiluminescence (PECL) was studied on photoanodes composed of Ir microbands on n-Si/SiOx. The results demonstrated that PECL microscopy accurately imaged the hole-driven heterogeneous photoelectrochemical reactivity. This method shows promise in elucidating the local activity of photoelectrodes used in solar energy conversion.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)